Prasetyo, Monika, Prasetyo, Monika and dr. Soehartono, Sp.T.H.T.K.L(K),, dr. Soehartono, Sp.T.H.T.K.L(K), and dr. Hendradi Surjotomo, Sp.T.H.T.K.L(K)., dr. Hendradi Surjotomo, Sp.T.H.T.K.L(K). (2019) Korelasi Ekspresi Interleukin-6 dengan Ekspresi Signal Transducer and Activator of Transcription 3 pada Mekanisme Metastasis Penderita KNF WHO Tipe III LMP-1 Positif”. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Karsinoma nasofaring adalah karsinoma sel skuamosa yang berasal dari epitel mukosa nasofaring. Karsinogenesis KNF yang kompleks disebabkan oleh interaksi antara infeksi kronis EBV, faktor lingkungan, dan perubahan genetik serta epigenetik. LMP-1 merupakan antigen dari EBV diekspresikan pada 65% kasus KNF. LMP-1 diyakini menstimulasi ekspresi sitokin yang mempengaruhi perilaku sel epitel salah satunya adalah IL-6. Aktivasi STAT3 melalui IL-6 pada sel tumor menyebabkan pertumbuhan tumor melalui kemampuan tumor menginvasi sistem imun. IL-6 akan mengaktivasi jalur STAT3 yang akan menginduksi invasi dan metastasis tumor. Metastasis merupakan proses akibat kemampuan adhesi menurun sehingga sebagian molekul dapat lepas dari matriks asal dan bermigrasi dari tumor primer ke jaringan lain. Peningkatan ekspresi IL-6 dan STAT3 memiliki kaitan erat dalam lingkungan mikro tumor penderita KNF, namun peran IL-6 dan STAT3 sendiri dalam modulasi aktivitas migrasi dan invasi sel KNF masih belum diketahui secara jelas. Tujuan: Mengetahui korelasi antara ekspresi IL-6 dan ekspresi STAT3 di jaringan nasofaring dengan mekanisme metastasis penderita KNF WHO tipe III LMP-1 positif. Metode: Penelitian observasional analitik dengan pendekatan cross-sectional yang melibatkan 15 penderita KNF WHO tipe III LMP-1 positif. Pemeriksaan ekspresi LMP-1, IL-6, dan STAT3 menggunakan metode pewarnaan imunohistokimia, dan hasilnya dihitung dengan menggunakan software Immunoratio. Penentuan stadium klinis metastasis berdasarkan pemeriksaan klinis dan penunjang radiologis lalu dievaluasi nilai T,N, dan M menggunakan AJCC 2017. Hasil: Analisis statistik ekspresi IL-6 dan STAT3 menunjukkan menunjukkan korelasi yang signifikan (p = 0.020) dengan koefisien korelasi r = 0.592 (Terdapat korelasi dengan arah korelasi positif). Terdapat perbedaan ekspresi IL-6 diantara penderita dengan stadium III, IVa, dan IVb namun tidak bermakna secara statistik (p=0,116). Terdapat perbedaan ekspresi STAT3 yang signifikan diantara penderita dengan stadium III, IVa, dan IVb (p=0,038). Kesimpulan: Semakin tinggi ekspresi IL-6 maka ekspresi STAT3 pada jaringan nasofaring penderita KNF WHO tipe III LMP-1 positif semakin meningkat. Semakin tinggi stadium klinis, maka ekspresi IL-6 dan STAT3 pada jaringan nasofaring penderita KNF WHO tipe III LMP-1 positif akan mengalami peningkatan. Namun peningkatan ekspresi IL-6 tidak bermakna secara statistik.
English Abstract
Background: Nasopharyngeal carcinoma is squamous cell carcinoma derived from the nasopharyngeal mucosal epithelium. Carcinogenesis of NPC is complex interactions between EBV infection, environmental factors, genetic and epigenetic. LMP-1 is EBV’s antigen expressed in 65% of NPC cases. LMP-1 is believed to stimulate expression of cytokines that affect the behavior of epithelial cells. Activation of STAT3 through IL-6 in tumor cells causes tumor growth through the ability of the tumor to invade the immune system. IL-6 will activate the STAT3 pathway which will induce tumor invasion and metastasis. Metastasis is a process due to decreased adhesion ability cause some molecules escaped from the original matrix and migrate from the primary tumor to other tissues. Increased expression of IL-6 and STAT3 has a close relationship in the tumor microenvironment of patients with NPC, but the role of IL-6 and STAT3 itself in modulation of migration activities and invasion of NPC cells is not fully understood. Objective: This study aims to determine the correlation between IL- 6 expression and STAT3 expression in nasopharyngeal tissue with metastatic mechanism of WHO type III LMP-1 positive NPC patients. Method: Observational analytic study with a cross-sectional approach involving 15 WHO type III LMP-1 positive NPC patients. Examination of LMP-1, IL-6, and STAT3 using immunohistochemical staining methods, and the results were calculated using software Immunoratio. Clinical staging of metastases based on clinical examination and radiological imaging then evaluated the staging with AJCC 2017. Result: Statistical analysis of IL-6 and STAT3 expressions showed a significant correlation (p = 0.020) with a correlation coefficient r = 0.592 (There was a positive correlation between them). There were differences in IL-6 expression among patients with stage III, IVa, and IVb but not statistically significant (p = 0.116). There were significant differences in STAT3 expression among patients with stage III, IVa, and IVb (p = 0.038). Conclusion: There is a significant positive correlation between IL-6 and STAT3 in tissue from WHO type III LMP-1 positive NPC patients. The higher the clinical stage, the expression of IL-6 and STAT3 in the nasopharyngeal tissue of positive WHO type III LMP-1 NPC patients will increase. However, the increase in IL-6 expression did not reach statistical significance
Other obstract
-
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.994/PRA/k/2019/041902163 |
| Uncontrolled Keywords: | Kata kunci: karsinoma nasofaring, Epstein-Barr Virus, Latent Membran Protein- 1, Interleukin-6,Signal Transducer and Activator of Transcription 3. |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.99 Tumors and miscellaneous communicable diseases > 616.994 Cancers |
| Divisions: | Profesi Kedokteran > Spesialis THT Kepala dan Leher, Fakultas Kedokteran |
| Depositing User: | yulia Chasanah |
| Date Deposited: | 12 Sep 2022 07:13 |
| Last Modified: | 12 Sep 2022 07:14 |
| URI: | http://repository.ub.ac.id/id/eprint/194153 |
![[thumbnail of Monika Teresa Prasetyo.pdf]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text
Monika Teresa Prasetyo.pdf Restricted to Registered users only Download (3MB) |
Actions (login required)
 |
View Item |