Parnidi, - and Prof. Dr. Ir. Lita Soetopo, - and Dr. Ir. Damanhuri,, MS and Dr. Drs. Marjani,, MP (2021) Analisis Genetik Dan Pewarisan Sifat Ketahanan Kenaf (Hibiscus Cannabinus L.) Terhadap Nematoda Puru Akar (Meloidogyne Incognita). Doktor thesis, Universitas Brawijaya.
Abstract
Kenaf (Hibiscus cannabinus L.) merupakan salah satu tanaman penghasil serat alami. Diversifikasi serat kenaf sudah banyak digunakan yaitu sebagai bahan pengemas, karpet, tekstil, fiber board (door-trim, interior mobil), partikel board, fibre drain, geo textile, kertas berkualitas tinggi, komposit, bahan baku pulp dan bahan tekstil otomotif. Banyak faktor yang menyebabkan kurang berkembangnya tanaman kenaf di Indonesia yaitu pada lahan subur tidak dapat bersaing harga dengan komoditas pangan. Oleh karena itu, pengembangan kenaf diarahkan ke lahan kering. Namun demikian pengembangan kenaf pada lahan kering terdapat beberapa kendala diantaranya adanya infeksi nematoda puru akar. Untuk mengatasi hal tersebut perlu dilakukan perakitan varietas unggul kenaf yang tahan nematoda puru akar. Upaya perakitan varietas unggul kenaf tahan nematoda puru akar memerlukan beberapa tahapan pemuliaan antara lain analisis pewarisan sifat. Analisis pewarisan sifat digunakan mendapatkan informasi tentang jumlah gen yang mengendalikan sifat tersebut, aksi gen yang mengendalikan, serta heritabilitasnya. Informasi tersebut sangat berguna dalam tahapan seleksi, sehingga seleksi dapat lebih efektif dan efisien. Pada tanaman kenaf informasi pewarisan sifat ketahanan terhadap nematoda puru akar masih perlu dikaji. Penelitian ini dilakukan di Kebun Percobaan Karangploso dan Laboratorium Ilmu Penyakit Tanaman, Balai Penelitian Tanaman Pemanis dan Serat (Balittas) Malang. Penelitian ini terdiri atas tiga tahap yaitu: (1) Evaluasi Ketahanan Beberapa Genotip Kenaf (Hibiscus cannabinus) Terhadap Nematoda Puru Akar (Meloidogyne incognita); (2) Analisis pewarisan sifat ketahanan kenaf terhadap NPA M. incognita; (3) Pendugaan parameter genetik ketahanan kenaf terhadap NPA M. incognita menggunakan analisis persilangan dialel. Berdasarkan hasil analisis pewarisan sifat didapatkan informasi bahwa ketahanan kenaf terhadap NPA M. incognita dikendalikan oleh satu gen yang bersifat dominan dengan aksi gen aditif. Oleh karena itu, kedepannya untuk perencanaan program pemuliaan ketahanan kenaf terhadap nematoda puru akar M. incognita dapat dilakukan dengan metode silang balik. Dengan menggunakan metode silang balik, untuk memindahkan satu gen yang bersifat dominan maka diperlukan paling sedikit enam generas
English Abstract
Kenaf (Hibiscus cannabinus L.) is a plant that produces natural fiber. Kenaf fiber diversification has been widely used, namely as a packaging material, carpets, textiles, fiber boards (door-trim, car interiors), particle board, fiber drain, geo textiles, high-quality paper, composites, pulp raw materials and automotive textiles. Therefore, kenaf development is directed to dry land. However, the development of kenaf on dry land has several obstacles, including the presence of root-knot nematode infection. To overcome this, it is necessary to assemble a superior variety of kenaf that can withstand root-knot nematodes. The effort to assemble high yielding varieties of root-knot nematodes resistant kenaf requires several breeding stages, including analysis of heredity. Inheritance analysis is used to obtain information about the number of genes controlling the trait, the action of the controlling genes, and their heritability. This information is very useful in the selection stage, so that selection can be more effective and efficient. In the kenaf plant, the inheritance information of the resistance to root-knot nematodes still needs to be studied. This research was conducted at the Karangploso Experimental Garden and the Laboratory of Plant Diseases, Indonesian Sweetener and Fiber Crops Research Institute-Malang. This study consisted of three stages, namely: (1) Resistance evaluation of several kenaf (Hibiscus cannabinus) genotypes Against Root Knot Nematodes (M. incognita); (2) Inheritance analysis of kenaf resistance to RKN M. incognita; (3) Estimation of genetic parameters of kenaf resistance to RKN M. incognita used dialellic cross analysis. Based on the results of inheritance analysis, it was found that the resistance of kenaf to root-knot nematodes (M. incognita) was controlled by one dominant gene with the gene action of additive. Therefore, in the future, planning the kenaf resistance breeding program against root-knot nematodes (M. incognita) can be done by using the back cross method. By using the back cross method, to transfer one dominant gene, at least six generations are required
Other obstract
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| Item Type: | Thesis (Doktor) |
|---|---|
| Identification Number: | 0621040007 |
| Uncontrolled Keywords: | aksi gen, heritabilitas, daya gabung, persilangan dialel, gene action, heritability, combining ability, diallel crosses. |
| Subjects: | 600 Technology (Applied sciences) > 630 Agriculture and related technologies |
| Divisions: | S2/S3 > Doktor Ilmu Pertanian, Fakultas Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 28 Jul 2022 03:14 |
| Last Modified: | 04 Oct 2024 01:43 |
| URI: | http://repository.ub.ac.id/id/eprint/192836 |
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