The Effect Of Using Maltose As Cryoprotectant On Semen Quality Of Taiwan Native Rooster During Frozen Storage

Effendi, Fredyan Isnan (2019) The Effect Of Using Maltose As Cryoprotectant On Semen Quality Of Taiwan Native Rooster During Frozen Storage. Magister thesis, Universitas Brawijaya.


Keberhasilan pembekuan sperma ayam diinginkan untuk melestarikan Taiwan ayam kampung. Sampel air mani dari tiga ayam jantan Taiwan dikumpulkan menggunakan teknik pijat dua kali seminggu. Dua Cryoprotectant Maltosa konsentrasi, 0,6 dan 1,2 g, ditambahkan dalam Tris Aminomethane+20 % telur kuning telur sebagai bahan pemanjang semen dasar. Tujuan dari penelitian ini adalah untuk mengamati efek pemanjang Tris Aminomethane yang dilengkapi dengan maltosa pada post kualitas thawing (motilitas spermatozoa massal, motilitas spermatozoa, abnormalitas spermatozoa, viabilitas dan integritas membran/uji HOST) selama penyimpanan beku pada spermatozoa Ayam Asli Taiwan

English Abstract

The success of freezing chicken semen is desired for preserving Taiwan native chickens. Semen samples from three Taiwan roosters were collected using massage technique twice a week. Two Cryoprotectant Maltose concentrations, 0.6 and 1.2 g, were added in Tris Aminomethane+20 % egg yolk as the basic semen extender. The purpose of this study was to observe the effect of Tris Aminomethane extender supplemented with maltose on post thawing quality (mass spermatozoa motility, spermatozoa motility, spermatozoa abnormality, viability and membrane integrity/HOST test) during frozen storage on Taiwan Native Rooster spermatozoa. The experiment was conducted in Animal Embriology Engineering Laboratory of III The National Pingtung University of Science and Technology, Taiwan. During the period of one month experiment, feed and water were provided ad libitum for the roosters. All semen samples were diluted (semen:diluent=1:10) with warmed diluents at 30ºC in a water bath. The diluted semensamples were gradually cooled to 4ºC for 4h. After cooling, spermatozoa motility was examined. The samples were packed in 0.25 ml straws and plugged with the straw powder. The straws were kept at 4ºC until frozen, which was carried out by placing the samples in liquid nitrogen vapor (-125 to -130ºC) for 20 minutes, and stored in liquid nitrogen (-196ºC) until examination after 3 hours. spermatozoamotility, abnormality and membrane integrity showed significant differences (P < 0.05) when preserved with cryoprotectant when compared to the control treatment. It can be concluded that maltose is a potential protector for improving spermatozoa quality during frozen storage

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Item Type: Thesis (Magister)
Identification Number: TES/636.508 2/EFF/t/2019/041905776
Uncontrolled Keywords: Cryoprotectant, maltose, Taiwan native rooster, tris aminomethane
Subjects: 600 Technology (Applied sciences) > 636 Animal husbandry > 636.5 Chickens and other kinds of domestic birds > 636.508 2 Chickens and other kinds of domestic birds (Breeding)
Divisions: S2/S3 > Magister Ilmu Ternak, Fakultas Peternakan
Depositing User: Endang Susworini
Date Deposited: 27 Jul 2022 08:44
Last Modified: 27 Jul 2022 08:44
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