Saputri, Ravanella Eryani and Prof. Dr. Ir. Arief Prajitno, MS and Budianto, S.Pi., M.P., M.Sc (2022) Uji daya hambat ekstrak kasar daun kitolod (Isotoma longiflora) terhadap bakteri Pseudomonas fluorescens secara in vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Permasalahan utama yang terjadi pada kegiatan budidaya salah satunya yaitu munculnya penyakit. Penyakit yang sering muncul dalam budidaya yaitu penyakit bakterial. Salah satu bakteri yang sering menginfeksi ikan yaitu bakteri P. fluorescens. Bakteri P. fluorescens merupakan bakteri patogen yang dapat merugikan berbagai spesies ikan baik ikan tawar maupun ikan air payau. Bakteri ini dapat juga mengakibatkan lesi hemoragik dan hemoragi petechial, dan lebih parahnya lagi dapat menyebabkan kematian massal pada komunitas ikan hingga mencapai 100%. Penanggulangan penyakit yang disebabkan P. fluorescens dapat dilakukan dengan pemberian antibiotik, namun penggunaan antibiotik yang berlebih dapat menimbulkan residu, resistensi bakteri dan pencemaran perairan budidaya. Pengurangan penggunaan antibiotik dapat dilakukan dengan cara mencari bahan alami sebagai pengganti antibiotik salah satunya daun kitolod. Daun kitolod mengandung senyawa flavonoid, tanin, saponin, dan alkaloid. Kandungan senyawa pada daun kitolod dapat menjadi antibakteri. Penelitian dilakukan di Laboratorium Sentral Ilmu Hayati (LSIH), Universitas Brawijaya, Malang. Penelitian dilaksanakan pada bulan Desember 2021- Januari 2022. Tujuan penelitian ini adalah untuk mengetahui daya hambat ekstrak kasar daun kitolod terhadap bakteri P. fluorescens secara in vitro . Metode penelitian yang digunakan yaitu metode eksperimental. Rancangan percobaan yang digunakan berupa Rancangan Acak Lengkap (RAL) dengan menggunakan 5 perlakuan, 3 ulangan dan 2 kontrol. Dosis ekstrak daun kitolod yang digunakan yaitu A (50 ppm), B (100 ppm), C (150 ppm), D (200 ppm), dan E (250 ppm). Kontrol positif menggunakan antibiotik tetracycline 30 ppm dan kontrol negatif tanpa perlakuan. Hasil penelitian menunjukkan bahwa bakteri P. fluorescens memiliki daya hambat terhadap setiap dosis ekstrak daun kitolod yang diberikan. Perlakuan dosis dengan rerata diameter zona hambat terbesar pada dosis E (250 ppm) sebesar 7,37 ± 0,55 mm sedangkan untuk rerata zona hambat terkecil pada dosis A (50 ppm) sebesar 6,06 ± 0,02 mm. Hubungan zona bening antar perlakuan ekstrak daun kitolod terhadap bakteri P. fluorescens menunjukkan perpotongan garis secara linier dengan persamaan y= 0,0058x + 5,87 dan koefisien R2= 0,6807 Kesimpulan yang didapat dari penelitian ini adalah ekstrak daun kitolod memiliki daya hambat terhadap bakteri P. fluorescens. Ekstrak daun kitolod memiliki sifat bakteriostatik, yaitu mampu menghambat pertumbuhan bakteri P. fluorescens. Perlakuan dosis tertinggi dalam menghambat pertumbuhan bakteri P. fluorescens yaitu E (250 ppm).
English Abstract
One of the main issues which mainly occur in cultivation is the emergence of diseases. One of the most common diseases in cultivation is bacteria-caused disease. P. fluorescens is one such bacterium that often infected fish. P. fluorescens is a harmful patogent for fish species either in fresh or saltwater. This bacterium causes boils and lesi hemoragic and hemoragi petechial, or even worse, high mass mortality in a community of fish which extend to 100% Antibiotics are some of the ways to prevent diseases caused by P. fluorescens, however, antibiotics could create residus, bacterial resistance and pollutants in cultivating waters. However, the use of antibiotics could be cut down by using natural materials instead of antibiotics, one of which is kitolod leaves. Kitolod Leaves contain flavonoid, tannin, saponin, and alkaloid compounds which could be highly anti-bacterial. This research was conducted in the Central Laboratorium Ilmu hayati (LSIH), University of Brawijaya, Malang. This study was conducted from December 2021 until January 2022. The purpose of this study is to understand the resistance of Kitolod leaf extract against P. fluorescens via in vitro. The research method was the experimental method. The experimental design used was a completely randomized design (CRD) using 5 treatments, 3 replications and 2 controls. The doses of kitolod leaf extract used were A (50 ppm), B (100 ppm), C (150 ppm), D (200 ppm), and E (250 ppm). Positive control used 30 ppm tetracycline antibiotics and negative control received no treatment. The results showed that P. fluorescens bacteria were resistance to each dose of kitolod leaf extract given. The treatment dose with the largest average diameter of the inhibition zone at dose E (250 ppm) was 7.37 ± 0.55 mm while the smallest inhibition zone average was at dose A (50 ppm) of 6.06 ± 0.02 mm. The clear zone relationship between kitolod leaf extract treatments and P. fluorescens bacteria showed a linear intersection with the equation y= 0.0058x + 5.87 and coefficient R2= 0.6807 The conclusion from this research is that kitolod leaf extract is resistance to P. fluorescens bacteria. Kitolod leaf extract has bacteriostatic properties, which is able to inhibit the growth of P. fluorescens bacteria. The highest dose treatment for resistance the growth of P. fluorescens was E (250 ppm).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522080060 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Budidaya Perairan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 26 Jul 2022 01:54 |
| Last Modified: | 26 Jul 2022 01:54 |
| URI: | http://repository.ub.ac.id/id/eprint/192668 |
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