Sherlyna, Fadilla and Tunjung Mahatmanto,, STP, M.Si, Ph.D and Mochamad Nurcholis,, STP, M.Si, Ph.D (2022) Analisis Genom Chryseobacterium sp. A99 - Bakteri Pendegradasi Bulu dari Tanah Perakaran Mimosa pudica. Sarjana thesis, Universitas Brawijaya.
Abstract
Bulu merupakan limbah organik yang mengandung lebih dari 90% keratin. Keratin merupakan protein dengan ikatan silang disulfida yang membuat bulu menjadi kuat sehingga sulit didegradasi. Keratin tersusun oleh α-heliks (α-keratin) atau β-sheet (β-keratin) yang membuat keratin sangat stabil. Meskipun demikian, limbah bulu tetap dapat didegradasi menggunakan metode mekanis, kimia, dan biologi. Namun, metode mekanis dan kimia membutuhkan energi yang besar, menimbulkan masalah lingkungan, dan dapat merusak asam amino esensial dalam keratin sehingga dapat mengurangi kualitas dari protein tersebut. Maka dari itu, metode terbaik untuk mendegradasi limbah bulu adalah dengan menggunakan enzim protease. Pada penelitian ini, digunakan Chryseobacterium sp. A99 yang menghasilkan enzim-enzim pendegradasi keratin. Bakteri Chryseobacterium sp. A99 dianalisa dengan metode Whole Genome Sequencing (WGS) dan dianalisa lebih lanjut menggunakan BLAST gen 16S rRNA dan Average Nucleotide Identity (ANI) Taxonomy Check untuk mengidentifikasi spesies bakteri. Kemudian, protein dan enzim yang berguna dalam degradasi keratin diidentifikasi dengan menganalisa Clusters of Orthologous Groups (COG) melalui database eggNOG. Berdasarkan hasil penelitian, ditemukan bahwa Chryseobacterium sp. A99 merupakan bakteri genus Chryseobacterium dengan level spesies baru. Chryseobacterium sp. A99 ditemukan memiliki protein serta enzim-enzim yang membantu dalam degradasi bulu. Protein yang membantu dalam degradasi bulu merupakan protein dengan fungsi untuk memfasilitasi adhesi bakteri terhadap bulu. Protein tersebut adalah putative auto-transporter adhesin (head GIN domain), collagen-binding protein dan fibronektin tipe 3 (fn3). Enzim-enzim yang berfungsi untuk mendegradasi bulu atau keratin adalah serine protease, ATP-dependent Clp protease ATP-binding subunit ClpX, putative neutral zinc metallopeptidase, rhomboid family intramembrane serine protease, neutral zinc metallopeptidase, protease I (pfpI), membrane protease subunit HflK (hflK), dan rhomboid protease GluP (gluP), piridin nukleotida-disulfida oksidoreduktase, protein-disulfida reduktase, tiol-disulfida oksidoreduktase, tiol reduktase tioredoksin, dan tiol-disulfida isomerase.
English Abstract
Feather is an organic waste that contains more than 90% keratin. Keratin is a protein containing disulfide bonds that make the feathers strong so they are difficult to be degraded. Keratin is made up of α-helix (α-keratin) or β-sheet (β-keratin) which causes keratin to be very stable. Even so, feathers could still be degraded using mechanical, chemical, and biological methods. However, mechanical and chemical methods require a lot of energy, cause environmental problems, could damage the essential amino acids in keratin and hence reduce the protein quality. Therefore, the best method to degrade feather waste is to use the protease enzymes. In this study, Chryseobacterium sp. A99 which is known to produce keratin-degrading enzymes was used. Chryseobacterium sp. A99 was then analyzed using the Whole Genome Sequencing (WGS) method and further analyzed using BLAST 16S rRNA gene and Average Nucleotide Identity (ANI) Taxonomy Check to identify its bacterial species. Then, proteins and keratin-degrading enzymes were identified by analyzing the Clusters of Orthologous Groups (COG) through the eggNOG database. Based on the results of the study, it was found that Chryseobacterium sp. A99 is a bacterium of the genus Chryseobacterium with a new species level. Chryseobacterium sp. A99 was found to have proteins as well as enzymes that help in the feathers degradation. Proteins that help in feathers degradation are proteins with a function to facilitate bacterial adhesion to feathers, namely putative auto-transporter adhesin (head GIN domain), collagen-binding protein and fibronectin type III (fn3). The keratin-degrading enzymes found are serine protease, ATP-dependent Clp protease ATP-binding subunit ClpX, putative neutral zinc metallopeptidase, rhomboid family intramembrane serine protease, neutral zinc metallopeptidase, protease I (pfpI), membrane protease subunit HflK (hflK), and rhomboid protease GluP (gluP), pyridine nucleotide-disulfide oxidoreductase, protein-disulfide reductase, thiol-disulfide oxidoreductase, thiol-disulfide oxidoreductase, and thiol-disulfide isomerase.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522100082 |
| Uncontrolled Keywords: | Chryseobacterium sp., limbah bulu, protease, sekuensing,Chryseobacterium sp., feather waste, protease, sequencing |
| Subjects: | 600 Technology (Applied sciences) > 630 Agriculture and related technologies |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 11 Jul 2022 06:51 |
| Last Modified: | 11 Jul 2022 06:51 |
| URI: | http://repository.ub.ac.id/id/eprint/191860 |
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