Irwanti, Siti Halimah Laili and drg.Nenny Prasetyaningrum,, M.ked (2020) Pengaruh Nanotransfersome Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia Swingle) terhadap Ekspresi Vascular Endothelial Growth Factor (VEGF) oleh Sel Makrofag pada Penyembuhan Luka Mukosa Labial Tikus Wistar. Sarjana thesis, Universitas Brawijaya.
Abstract
Latar Belakang : Proses penyembuhan ulkus dipengaruhi oleh Vascular Endothelial Growth Factor (VEGF) yang berfungsi sebagai faktor proangiogenik. Nanotransfersome ekstrak kulit jeruk nipis (Citrus aurantifolia Swingle) mengandung flavonoid diharapkan mampu meningkatkan penetrasi obat Tujuan : Mengetahui pengaruh pemberian nanotransfersome ekstrak kulit jeruk nipis terhadap jumlah ekspresi vascular endothelial growth factor (VEGF) oleh sel makrofag pada penyembuhan luka mukosa labial tikus wistar. Metode : metode penelitian true experimental in vivo dengan induksi termal pada mukosa labial tikus wistar dan dibagi 4 kelompok perlakuan, yaitu K(-) (tanpa perlakuan), K(+) (perlakuan triamcinolone acetonide 0.1%), PA (perlakuan ekstrak kulit jeruk), dan PB (perlakuan nanotransfersome ekstrak kulit jeruk nipis). Pengamatan penyembuhan luka dilakukan pada hari ke-3 dan ke-7 dengan pewarnaan imunohistokmia. Jumlah ekspresi VEGF oleh sel makrofag dihitung menggunakan mikroskop digital dengan perbesaran 1000x dalam 10 lapang pandang. Hasil : Kelompok nanotransfersome menunjukkan rata-rata jumlah ekspresi VEGF yang paling tinggi. Analisis data dengan uji One-way ANOVA menujukkan perbedaan ekspresi VEGF yang signifikan antar kelompok pada hari ke-3 maupun ke-7. Kesimpulan : Nanotransfersome ekstrak kulit jeruk nipis berpengaruh terhadap jumlah ekspresi VEGF oleh sel makrofag pada penyembuhan luka mukosa labial tikus wistar.
English Abstract
Background: The process of healing ulcers is influenced by Vascular Endothelial Growth Factor (VEGF) which functions as a proangiogenic factor. Nanotransfersome extract of lime peel (Citrus aurantifolia Swingle) containing flavonoids is expected to be able to increase drug penetration. Purpose: To determine the effect of nanotransfersome lime peel extract on VEGF expression by macrophage cells in wound healing of labial mucosa wistar rats. Methods: True experimental research method in vivo with thermal induction in the labial mucosa of wistar rats and divided into 4 treatment groups, namely K (-) (without treatment), K (+) (triamcinolone acetonide 0.1% treatment), PA (treatment of orange peel extract ), and PB (nanotransfersome treatment of lime peel extract). Observation of wound healing is done on the 3rd and 7th day by staining immunohistokmia. The amount of VEGF expression in macrophage cells was calculated using a digital microscope at 1000x magnification in 10 fields of view. Results: the nanotransfersome group showed the highest average VEGF expression. Data analysis using the One-way ANOVA test showed that there were significant differences in VEGF expression between groups on the 3rd and 7th day. Conclusion: Nanotransfersome lime peel extract affected VEGF expression by macrophage cells in wound healing of labial mucosa wistar rats.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520140093 |
| Uncontrolled Keywords: | Nanotransfersome ekstrak kulit jeruk nipis (Citrus aurantifolia Swingle), flavonoid, ulkus traumatik, VEGF,Nanotransfersome lime peel extract (Citrus aurantifolia Swingle), flavonoid, traumatic ulcers, VEGF. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 05 Jul 2022 03:48 |
| Last Modified: | 05 Jul 2022 03:48 |
| URI: | http://repository.ub.ac.id/id/eprint/191556 |
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