Tanujaya, Andry Anthony and drg. Dini Rachmawati,, Sp. KGA. (2022) Pengaruh Pemberian Limbah Cair Tahu terhadap Jumlah Sel Neutrofil dan Limfosit pada Tikus Wistar (Rattus Norvegicus) Model Periodontitis. Sarjana thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Periodontitis adalah penyakit peradangan kronis periodonsium dan ditandai dengan kehilangan ligamen periodontal dan kerusakan tulang alveolar. Selama ini, penanganan periodontitis sering kali diikuti dengan obat-obatan kimiawi. Pengobatan kimiawi memiliki banyak efek samping bagi tubuh, maka perlu dikembangkan obat herbal alternatif seperti limbah cair tahu. Diperkirakan isoflavon terdapat dalam limbah cair tahu yang terbuang selama proses pembuatan. Tujuan: Untuk mengetahui pengaruh limbah cair tahu terhadap jumlah sel neutrofil dan limfosit pada mandibula tikus wistar model periodontitis. Metode: Penelitian ini menggunakan 5 kelompok tikus wistar yaitu: K(0) (tanpa perlakuan), K- (perlakuan bakteri Pg 0,03 ml 3 hari sekali), P-1 (perlakuan bakteri Pg 0,03 ml 3 hari sekali + limbah cair tahu 6ml/kgBB), P-2 (perlakuan bakteri Pg 0,03 ml 3 hari sekali + limbah cair tahu 12ml/kgBB), dan P-3 (perlakuan bakteri Pg 0,03 ml 3 hari sekali + limbah cair tahu 18ml/kgBB). Setelah 28 hari, dilakukan pengambilan tulang alveolar untuk pembuatan preparat HE. Pengamatan sel neutrofil dan limfosit dilakukan dengan mikroskop cahaya dengan perbesaran 400x dengan 5 lapang pandang. Hasil: Uji Kruskall-Wallis dan uji post hoc Mann-Whitney menunjukkan adanya pengaruh pemberian limbah cair tahu terhadap jumlah sel neutrofil (p<0,05). Uji One Way Anova dan uji post hoc LSD menunjukkan adanya pengaruh pemberian limbah cair tahu terhadap jumlah sel limfosit (p<0,05). Kesimpulan: Pemberian limbah cair tahu berbagai dosis, terutama dosis 18ml/kg memiliki pengaruh yang signifikan terhadap penurunan jumlah sel neutrofil dan limfosit pada tikus wistar model periodontitis.
English Abstract
Background: Periodontitis is a chronic inflammatory disease of the periodontium and characterized by loss of the periodontal ligament and destruction of the alveolar bone. During this time, the treatment of periodontitis is often followed by chemical drugs. Chemical treatment has many side effects, it is necessary to develop herbal medicines such as tofu liquid waste. It is estimated that isoflavones are present in tofu liquid waste that is wasted during the manufacturing process. Objective: To determine the effect of tofu liquid waste on the number of neutrophil and lymphocyte cells in the mandible of wistar rats periodontitis model. Methods: This study used 5 groups of wistar rats, namely: K(0) (without treatment), K- (treatment of 0.03 ml Pg bacteria every 3 days), P-1 (treatment of 0.03 ml Pg bacteria every 3 days + tofu liquid waste 6ml/kgBW), P-2 (treatment of 0.03 ml Pg bacteria every 3 days + tofu liquid waste 12ml/kgBW), and P-3 (treatment of 0.03 ml Pg bacteria every 3 days + tofu liquid waste 18ml/kgBW). ). After 28 days, the alveolar bone was taken HE preparations. Observations of neutrophil and lymphocyte cells were carried out by light microscope with 400x magnification with 5 fields of view. Results: Kruskall-Wallis test and post hoc Mann-Whitney test showed that there was an effect of giving tofu liquid waste on the number of neutrophil cells (p<0.05). One Way Anova test and post hoc LSD test showed that there was an effect of giving tofu liquid waste on the number of lymphocyte cells (p<0.05). Conclusion: The distribution of tofu liquid waste at various doses, especially 18ml/kgBW, had a significant effect on reducing the number of neutrophil and lymphocyte cells in wistar rats periodontitis model.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522140002 |
| Uncontrolled Keywords: | Periodontitis, Limbah Cair Tahu, Isoflavon, Anti-inflamasi,Periodontitis, Tofu Liquid Waste, Isoflavones, Anti-inflammation. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 01 Jul 2022 07:38 |
| Last Modified: | 01 Jul 2022 07:38 |
| URI: | http://repository.ub.ac.id/id/eprint/191422 |
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Text (DALAM MASA EMBARGO)
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