Anggiarta, Kevin Satria and drg . Viranda Sutanti ,, M.Si. (2020) Analisis Efek Ekstrak Kulit Pisang Kepok Kuning ( Musa paradisiaca L. ) Terhadap Ukuran Dan Morfologi Bakteri Enterococcus faecalis Menggunakan Scanning Electron Microscope ( SEM ). Sarjana thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Penyakit pulpa dan periapikal merupakan penyakit yang seringkali mengalami kegagalan perawatan dikarenakan menetapnya infeksi pada pulpa dan periapikal. Bakteri yang dominan dalam proses infeksi tersebut adalah Enterococcus faecalis. Penggunaan antimikroba beralih ke bahan alami yang memiliki toksisitas rendah, murah serta memiliki sifat tahan lama. Kulit pisang kepok kuning memiliki banyak senyawa antibakteri seperti flavonoid, tannin alkaloid serta mudah ditemukan di Indonesia. Tujuan: Mengetahui gambaran perubahan morfologi dan ukuran bakteri Enterococcus faecalis setelah pemberian ekstrak kulit pisang kepok kuning (Musa paradisiaca L.) secara In vitro. Metode: Sampel konsentrasi ekstrak kulit pisang kepok kuning 100%, 90%, 80%, 70%, 60% dan kontrol ditempat kan pada well yang terdapat coverslip. Tiap well diberikan BHI-B dan bakteri Enterococcus faecalis. Coverslip pada 5 konsentrasi dilakukan pelapisan menggunakan emas dan akan dilakukan foto menggunakan Scanning Electron Microscope (SEM). Hasil foto kemudian diamati panjang serta morfologi pada bakteri. Data dianalisis dengan statistik deskriptif. Hasil: Rata – rata ukuran panjang bakteri yang diberikan ekstrak meningkat daripada bakteri yang tidak diberikan ekstrak sedangkan rata – rata ukuran lebar bakteri menunjukkan perubahan yang tidak menentu. Pada bakteri yang diberikan ekstrak menunjukkan perubahan morfologi pada bakteri berupa tonjolan (blebs). Kesimpulan: Ekstrak kulit pisang kepok kuning (Musa paradisiaca L.) memberikan gambaran perubahan morfologi dan ukuran bakteri Enterococcus faecalis secara in vitro.
English Abstract
Background: Pulp and periapical disease are diseases that often fail at treatment due to persistent infection of the pulp and periapical. The dominant bacterium in the infection process is Enterococcus faecalis. The use of antimicrobials switches to natural ingredients which have low toxicity, are inexpensive and have long-lasting properties. Yellow kepok banana peels have many antibacterial compounds such as flavonoids, tannin alkaloids and are easily found in Indonesia. Objective: To determine the morphological changes and size of Enterococcus faecalis bacteria after application of yellow kepok banana peel extract (Musa paradisiaca L.) in vitro. Method: 100%, 90%, 80%, 70%, 60% yellow banana peel extract concentration and control were placed on wells with coverslip. Each well was given BHI-B and Enterococcus faecalis bacteria. Coverslips at 5 concentrations are coated using gold and photographs will be taken using a Scanning Electron Microscope (SEM). The results of the photograph were then observed in length and morphology in bacteria. Data were analyzed with descriptive statistics. Results: The average length of the bacteria given the extract increased compared to the bacteria that were not given the extract while the average size of the bacterial width showed an erratic change. Extracted bacteria showed morphological changes in bacteria in the form of protrusions (blebs). Conclusion: Yellow kepok banana peel extract (Musa paradisiaca L.) provides an overview of changes of morphological and the size of Enterococcus faecalis bacteria in vitro
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520140055 |
| Uncontrolled Keywords: | Enterococcus faecalis, Kulit Pisang Kepok Kuning, Perubahan Ukuran dan Morfologi,Enterococcus faecalis, Yellow Kepok Banana Peels, Change Size and Morphology |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 17 Jun 2022 06:57 |
| Last Modified: | 17 Jun 2022 06:57 |
| URI: | http://repository.ub.ac.id/id/eprint/191293 |
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Text (DALAM MASA EMBARGO)
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