Nugraha, Ibnu Putra and drg. Fredy Mardiyantoro, Sp.BM and dr. Novi Khila Firani,, M. Kes., Sp.PK. (2019) Pengaruh Gelatin Ikan Patin (Pangasius djambal) Terhadap Jumlah Limfosit pada Luka Pasca Pencabutan Gigi Tikus Putih (Rattus norvegicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Salah satu fase dalam penyembuhan luka adalah fase inflamasi. Limfosit merupakan sel pusat dari sistem imunitas yang mempunyai peranan penting dalam proses inflamasi. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah gelatin. Tujuan penelitian adalah untuk mengetahui pengaruh pemberian gelatin ikan patin (Pangasius djambal) terhadap jumlah limfosit pada proses penyembuhan luka pasca pencabutan gigi tikus wistar putih (Rattus norvegicus). Desain penelitian adalah eksperimental murni dengan Post Test Only Randomized Control Group Design secara in-vivo. Gelatin ikan patin dibuat dengan metode modifikasi tanpa proses pengeringan. Penghitungan sel imfosit secara manual menggunakan mikroskop digital dengan perbesaran 400x yang didapat dari preparat soket alveolar tikus Wistar. Identifikasi sel limfosit dilakukan dengan pewarnaan HE. Hasil penelitian, uji One Way ANOVA menunjukkan terdapat perbedaan yang signifikan antara kelompok perlakuan yang menggunakan gelatin ikan patin dengan kelompok kontrol. Uji Korelasi Pearson menunjukkan korelasi negative antara variabel penelitian, dimana semakin rendah hasil hitung limfosit pada kelompok kontrol (K), maka semakin tinggi hasil hitung limfosit pada kelompok perlakuan (P). Disimpulkan bahwa pemberian ekstrak gelatin ikan patin berpengaruh terhadap peningkatan jumlah sel limfosit pada proses penyembuhan luka pasca pencabutan gigi tikus wistar putih.
English Abstract
One phase in wound healing is the inflammatory phase. Lymphocytes are the central cells of the immune system which have an important role in the inflammatory process. One of the natural substance that can help the wound healing process is gelatin. The aim of the study was to determine the effect of catfish gelatin (Pangasius djambal) on the number of lymphocytes in the wound healing process after the extraction of white wistar mice teeth (Rattus norvegicus). The design of the study was experimental purely with Post Test Only Randomized Control Group Design in vivo. Catfish gelatin was made by the modified method from without drying process. Limfocytes was counted manually by a digital microscope with 400x magnification, the preparation obtained from Wistar alveolar socket. Identification of lymphocyte cells was done by coloring HE. The results of the study, the One Way ANOVA test showed that there was a significant difference between the treatment groups using catfish gelatin and the control group. Pearson Correlation Test showed a negative correlation between the research variables, where the lower the lymphocyte count results in the control group (K), the higher the lymphocyte count results in the treatment group (P). It was concluded that the administration of catfish gelatin extract had an effect on the increase in the number of lymphocyte cells in the wound healing process after extracting white wistar teeth.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0519140003 |
| Uncontrolled Keywords: | penyembuhan luka, inflamasi, limfosit, gelatin, ikan patin,wound healing, inflammation, lymphocytes, gelatin, catfish. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 14 Jun 2022 06:39 |
| Last Modified: | 14 Jun 2022 06:39 |
| URI: | http://repository.ub.ac.id/id/eprint/191115 |
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