Susanto, Jefri Pratama and DR. dr. Nursamsu, SpPD-KGH and dr. Rulli Rosandi, SpPD-KEMD (2022) Pengaruh Pemberian Sylibin dan Telmisartan Terhadap Kadar Nefrin Urin Tikus Diabetes. Magister thesis, Universitas Brawijaya.
Abstract
LATAR BELAKANG: Diabetes Melitus (DM) merupakan penyakit metabolik dan endokrin yang memiliki dampak serius terhadap kesehatan. Komplikasi DM yang paling sering terjadi salah satunya yaitu nefropati diabetik. Podosit merupakan sel yang paling penting dalam patogenesis nefropati diabetic. Kerusakan podosit ditandai dengan kadar nefrin urin yang tinggi. Silybin dan telmisartan merupakan senyawa yang dapat mencegah kerusakan podosit ini melalui berbagai jalur. TUJUAN PENELITIAN: Mengetahui pengaruh pemberian silybin (SB), telmisartan ™ dan kombinasi silybin dan telmisaran (SB + TM) terhadap kadar nefrin urin tikus model nefropati diabetic METODE: Penelitian ini merupakan penelitian eksperimental laboratorik dengan desain true experimental laboratory dan metode randomized posttest only controlled group design. Subjek penelitian adalah tikus jantan strain Wistar yang memenuhi kriteria inklusi. Subjek terbagi menjadi 5 kelompok yaitu kelompok kontrol negative, kelompok kontrol positif, kelompok perlakuan SB, kelompok perlakuan TM dan kelompok perlakuan SB + TM. Pengukuran kadar nefrin menggunakan metode ELISA. Uji statistic dengan analis uji normalitas data, uji homogenitas varian, uji One-way ANOVA, dan Post hoc test (uji Least Significant Difference) dan uji korelasi Pearson. HASIL: Terdapat 20 tikus yang memenuhi kriteria inklusi. Uji T berpasangan menunjukkan bahwa didapatkan perbedaan bermakna antara kadar albumin urin kelompok kontrol positif sebelum perlakuan dengan kelompok kontrol positif sesudah perlakuan (p = 0.000) dan juga didapatkan perbedaan bermakna antara kadar nefrin urin kelompok kontrol negative dibandingkan dengan kelompok kontrol positive (p = 0.034) dan didapatkan korelasi positif antara kadar albumin urin dengan kadar nefrin urin (p = 0.035; r = 0.385) KESIMPULAN: Terdapat korelasi positif antara kadar albumin urin dengan kadar nefrin urin. Pemberian silybin atau telmisartan secara monoterapi, atau kombinasi silybin dan telmisartan tidak signifikan dalam menurunkan nefrin urin pada tikus diabetes.
English Abstract
BACKGROUND: Diabetes Mellitus (DM) is a metabolic and endocrine disease that has a serious impact on health. One of the most common complications of DM is diabetic nephropathy. Podocytes are the most important cells in the pathogenesis of diabetic nephropathy. Podocyte damage is characterized by high urinary nephrin levels. Silybin and telmisartan are compounds that can prevent damage to these podocytes through various pathways. OBJECTIVE: To determine the effect of silybin (SB), telmisartan (TM) and the combination of silybin and telmisaran (SB + TM) on urinary nephrin levels in diabetic nephropathy model rats. METHODS: This study is an experimental laboratory study with a true experimental laboratory design and a randomized posttest only controlled group design method. The research subjects were male Wistar rats that met the inclusion criteria. Subjects were divided into 5 groups, namely negative control group, positive control group, SB treatment group, TM treatment group and SB + TM treatment group. Nephrine levels were measured using the ELISA method. Statistical test with analysis of data normality test, homogeneity of variance test, Oneway ANOVA test, and Post hoc test (Least Significant Difference test) and Pearson correlation test. RESULTS: There were 20 mice that met the inclusion criteria. Paired T-test showed that there was a significant difference between urine albumin levels in the positive control group before treatment and the positive control group after treatment (p = 0.000) and there was also a significant difference between urine nephrin levels in the negative control group and the positive control group (p = 0.034). and found a positive correlation between urine albumin levels and urinary nephrin levels (p = 0.035; r = 0.385) CONCLUSION: There is a positive correlation between urine albumin level and urinary nephrin level. Administration of silybin or telmisartan as monotherapy, or the combination of silybin and telmisartan was not significant in reducing urinary nephrin in diabetic rat
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0422060005 |
| Uncontrolled Keywords: | Diabetes melitus, silybin, telmisartan, nefrin urin |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 10 Jun 2022 09:19 |
| Last Modified: | 10 Jun 2022 09:19 |
| URI: | http://repository.ub.ac.id/id/eprint/190962 |
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