Poerwosusanta, Hery (2019) Ekstrak Bulbus (Umbi) Bawang Dayak (Eleutherine Americana L. Merr.) Menurunkan Skoring Adesi Intra Abdominal Pasca Laparoskopi (Melalui Mekanisme Modulasi Inflamasi, Modulasi Stres Oksidatif Dan Stabilisator Sel Mast). Doctor thesis, Universitas Brawijaya.
Abstract
Adesi intra-abdominal adalah perlengketan tidak normal berbentuk pita dengan vaskularisasi pada organ intra abdomen. Laparoskopi pembedahan minimal invasive mulai mengantikan laparotomy. Laparoskopi belum mampu menurunkan adesi intra-abdominal. Diduga sel mast berperan pada adesi intraabdominal. Perlu dilakukan penelitian tentang peran sel sel mast pada adesi intra-abdominal. Penelitian ini bertujuan membuktikan pengaruh tekanan pada sel mast pada adesi intra-abdominal, serta membuktikan efek ekstrak bawang Dayak terhadap sel mast dan adesi intra-abdominal. Dengan rancangan post test only control group design, memenuhi syarat etik penelitihan hewan, dilakukan penelitian pada 3 tahap penelitian menggunakan 108 hewan coba rattus norvegicus membanding profil inflamasi, profil stres oksidatif, profil sel mast, profil matrik ekstra selular peritoneal, skoring adesi intra-abdominal dan perubahan mikroskopis. Penelitian bertujuan membuktikan hipotesis yaitu ada perbedaan skoring adesi intra-abdominal pada kelompok kontrol dan kelompok perlakuan dengan pemberian ekstrak umbi (bulbus) bawang Dayak. Pada penelitian tahap 1 insiliko ekstrak bawang Dayak berpotensi besar sebagai penstabil sel mast melalui regulasi ion Ca2+ terutama pada Ca2+ kanal mitokondria VDAC1 dan MARCK (mRyR). Pada penelitian tahap 2 terbukti tekanan laparoskopi 10 mmHg meningkatkan skoring adesi intra-abdominal akibat peningkatan kadar TGF-β, peningkatan kadar TOS dan OSI, peningkatan jumlah dan degranulasi sel mast dengan peningkatan mediator histamine, tryptase, peningkatan ekspresi zone occludine-1, peningkatan ketebalan MES dan perubahan mikroskopis peritoneum (p<0.05). Pada penelitian tahap 3 terbukti pemberian ekstrak umbi (bulbus) bawang Dayak 60 mg/KgBB mampu menurunkan skoring adesi intra-abdominal, menurunkan TGF-β, menurunkan kadar TOS dan OSI, menurunkan jumlah dan degranulasi sel mast dengan penurunan histamine, tryptase dan perubahan mikrokopis peritoneum (p<0.05). Kesimpulan: Ekstrak umbi bawang Dayak diprediksi menstabilkan sel mast melalui Ca2+ homeostasis. Tekanan 10 mmHg meningkatkan jumlah dan degranulasi sel mast serta meningkatkan skoring adesi intra-abdominal. Pemberian ekstrak umbi (bulbus) bawang Dayak 60 mg/KgBB menurunkan jumlah dan degranulasi sel mast serta menurunkan skoring adesi intraabdominal.
English Abstract
Intra-abdominal adhesion is an abnormal vascularized ribbon-shaped adhesion of intra-abdominal organs. Laparoscopic preferred than laparotomy, because of the ability to reduce intra-abdominal adhesion. It suspected that mast cells play an essential role in intra-abdominal adhesion. Research needs about the role of mast cells in intra-abdominal adhesion. This study aims to prove the effect of pressure on mast cells in intra-abdominal adhesion and to prove the effect of Dayak extracts on mast cells and intra-abdominal adhesion. With a post-test only control group design, our study was performed at three stages of the study using 108 Rattus norvegicus animals comparing the inflammatory profile, oxidative stress profile, mast cell profile, peritoneal extracellular matrix profile, scoring of intra-abdominal adhesion and microscopic peritoneal changes. Proved the hypothesis that there are differences in the degree of intra-abdominal adhesion in the control group and the treatment group after administering Dayak onion bulb extract. Insilico study proved that Dayak onion extract has excellent potential as a mast cell stabilizer through regulation of Ca2+ ions, especially in mitochondrial canal Ca2+ VDAC1 and MARCK (mRyR). The 2nd phase, a 10 mmHg laparoscopic increased the scoring of intra-abdominal adhesions due to elevated TGF-β levels, increased levels of TOS and OSI, increased number and degranulation of mast cells with increased histamine, tryptase, chymase, increased expression of zone occludine-1, increase in MES thickness and peritoneal microscopic changes (p <0.05). The 3rd phase, it proved that Dayak onion bulb extract of 60 mg/kg-BW was able to reduce intra-abdominal adhesion scoring, reduce TGF-β, reduce levels of TOS and OSI, reduce the number and degranulation of mast cells with a decrease in histamine, tryptase and microscopies change peritoneum (p <0.05). Conclusion: Dayak onion bulb extracts were predicted to stabilize mast cells through the Ca2+ homeostasis. Laparoscopic in 10 mmHg increases the number and mast cell degranulation and increases the scoring of intra-abdominal adhesions. Dayak onion bulb extracts 60 mg/kg BW decreases the number and mast cell degranulation and decreases the scoring of intra-abdominal
Other obstract
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| Item Type: | Thesis (Doctor) |
|---|---|
| Identification Number: | DIS/615.323 38/POE/e/2019/062000483 |
| Uncontrolled Keywords: | Bawang Dayak, Ca2+ homeostasis, regulasi inflamasi, regulasi stres oksidatif, sel mast, degranulasi sel mast, adesi intra-abdominal. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 34 drugs derived from specific plants (ranunculales) |
| Divisions: | S2/S3 > Doktor Ilmu Kedokteran, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 27 Jan 2022 02:44 |
| Last Modified: | 27 Jan 2022 02:44 |
| URI: | http://repository.ub.ac.id/id/eprint/188934 |
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