Kurniawati, Della Dwi and drh. Rahadi Swastomo, M.Biomed (2021) Studi Eksperimental Induksi Bakteri Enterobacter cloacae Dan Escherichia coli Pada Mencit Balb/C Yang Terinfeksi Plasmodium berghei Terhadap Perubahan Histopatologi Limpa Dan Jantung. Sarjana thesis, Universitas Brawijaya.
Abstract
Malaria merupakan penyakit yang disebabkan oleh Plasmodium sp. yang dapat menyerang reptil, burung, dan mamalia (manusia, primata, dan rodensia) melalui vektor nyamuk Anopheles sp,. Plasmodium sp. menyerang eritrosit dan memicu terjadinya kerusakan multiorgan. Penelitian terdahulu menyatakan bahwa koinfeksi bakteri Enterobacter cloacae dan Escherichia coli mampu menghambat aktivitas Plasmodium pada midgut nyamuk, sehingga pada penelitian ini bertujuan untuk mengetahui efek pengaruh infeksi bakteri Enterobacter cloacae dan Escherichia coli terhadap histopatologi organ limpa dan jantung pada mencit Balb/C yang diinfeksi Plasmodium berghei. Induksi Plasmodium berghei dilakukan secara intraperitoneal 0,2 ml dengan konsentrasi 107 sel parasit/ml pada hari ke-8. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 20 ekor mencit Balb/C jantan berumur 8 minggu dan memiliki berat badan rata-rata 20 gram yang terbagi menjadi 5 kelompok dengan 4 ulangan, yaitu kontrol negatif (KN) yaitu mencit Balb/C tidak diberikan perlakuan, kontrol positif (KP) yaitu mencit Balb/C diinfeksi Plasmodium berghei 0,2 ml dengan konsentrasi 107 sel parasit/ml, kelompok perlakuan 1 (P1) yaitu mencit Balb/C diinfeksi Plasmodium berghei dan Enterobacter cloacae 0,1 ml, kelompok perlakuan 2 (P2) yaitu mencit Balb/C diinfeksi Plasmodium berghei dan Escherichia coli 0,1 ml, dan kelompok perlakuan 3 (P3) yaitu mencit Balb/C diinfeksi Plasmodium berghei dan kombinasi Enterobacter cloacae dan Escherichia coli masing-masing 0,1 ml. Derajat parasitemia dihitung 7 hari pasca induksi bakteri dan mencit dieuthanasi untuk preparasi organ limpa dan jantung. Gambaran histopatologi organ limpa dan jantung diamati secara deskriptif komparatif. Hasil penelitian menunjukkan bahwa pemberian bakteri Enterobacter cloacae, Escherichia coli, dan kombinasi Enterobacter cloacae serta Escherichia coli mampu menurunkan tingkat kerusakan limpa dengan mengurangi pelebaran pulpa putih, nekrosis, dan pigmentasi serta mampu menurunkan tingkat kerusakan jantung dengan mengurangi infiltrasi sel radang dan nekrosis pada mencit Balb/C yang terinfeksi Plasmodium berghei. Kesimpulan dari penelitian ini adalah kombinasi bakteri Enterobacter cloacae dan Escherichia coli mampu memperbaiki kerusakan histopatologi limpa dan jantung mencit Balb/C yang terinfeksi Plasmodium berghei.
English Abstract
Malaria is a disease caused by Plasmodium sp. that could attack reptiles, birds, mammals (humans, primates, and rodents) through Anopheles sp., mosquito vector. Plasmodium sp attack erythrocytes and trigger multi-organ damage. Previous research stated that the coinfection of Enterobacter cloacae and Escherichia coli bacteria was able to inhibit Plasmodium activity in mosquito midgut, so this study aims to determine the effect of infection with Enterobacter cloacae and Escherichia coli on the histopathology of spleen and heart organs in Balb/C mice infected with Plasmodium berghei. Plasmodium berghei 0,2 ml induction was performed intraperitoneally with concentration 107 parasite cells/ml on day 8. This study used a complete randomized design with 20 male Balb/C mice aged 8 weeks and had an average body weight of 20 grams which were divided into 5 groups with 4 replications, namely negative control (KN) is Balb/C mice not given treatment, positive control (KP) is Balb/C mice infected with concentration 107 parasite cells/ml of Plasmodium berghei, treatment group 1 (P1) is Balb/C mice infected with Plasmodium berghei and Enterobacter cloacae 0,1 ml, treatment group 2 (P2) is Balb/C mice were infected with 0,1 ml Plasmodium berghei and Escherichia coli, and treatmen group 3 (P3) is Balb/C mice infected with Plasmodium berghei and combination of Enterobacter cloacae and Escherichia coli respectively 0,1 ml. the degree of parasitemia was calculated 7 days after bacterial induction and the mice were euthanized for spleen and heart organs preparation. The histopathological features of the spleen and heart were observed in a comparative descriptive manner. The result showed that giving the bacteria Enterobacter cloacae, Escherichia coli, and a combination of Enterobacter cloacae and Escherichia coli was able to reduce the level of spleen damage by reducing white pulp dilation, necrosis, and pigmentation and was able to reduce the level of heart damage by reducing inflammatory cell infiltration and necrosis in mice Balb/C infected with Plasmodium berghei. The conclusion of this study was that the combination of bacteria Enterobacter cloacae and Escherichia coli were able to repair the histopathological damage of the spleen and heart of Balb/C mice infected with Plasmodium berghei.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0521130051 |
| Uncontrolled Keywords: | Kata Kunci : Plasmodium berghei, Mencit Balb/C, Limpa, Jantung, Enterobacter cloacae, Escherichia coli. |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | yulia Chasanah |
| Date Deposited: | 25 Jan 2022 06:57 |
| Last Modified: | 28 Jun 2022 03:42 |
| URI: | http://repository.ub.ac.id/id/eprint/188821 |
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