Rahmadhan, Deva Fernanda and drh. Dian Vidiastuti, M.Si. (2021) Perbedaan Jumlah Sel Radang dan Sel Fibroblas Pada Lambung Tikus Putih (Rattus norvegicus) Pasca Gastrotomi yang Diberi Terapi Kombinasi Kunyit dan Madu. Sarjana thesis, Universitas Brawijaya.
Abstract
Gastrotomi merupakan prosedur pembedahan pada organ lambung dengan menginsisi bagian kurvatura mayor hingga lumen. Pemberian terapi kunyit dan madu diketahui memiliki khasiat dalam proses kesembuhan luka. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah sel radang dan sel fibroblas pada gambaran histopatologi lambung tikus pasca gastrotomi yang diberi terapi kunyit madu. Jenis penelitian ini merupakan penelitian true experimental. Sampel penelitian berupa tikus putih galur Sprague-Dawley jantan berumur 3 bulan dengan berat 400 gram sebanyak 25 ekor. Tikus dibagi menjadi 5 kelompok perlakuan dengan masing-masing kelompok berisi 5 ekor tikus. Pemberian perlakuan berupa (K-) tidak diberi terapi, (K+) omeprazole 20 mg/KgBB, (P1) kunyit 500 mg/KgBB, (P2) madu 0,333 ml/ml, (P3) kombinasi kunyit 250 mg/KgBB + madu 0,5 ml/ml secara PO 1x sehari selama 10 hari. Sampel lambung diproses menggunakan pewarnaan Hematoxylin-Eosin (HE) dan diamati menggunakan mikroskop perbesaran 400x. Pengambilan lima lapang pandang dengan metode Purposive Sampling. Analisa data kuantitatif menggunakan uji Kruskal-Wallis dengan tingkat kepercayaan 95% dan dilanjutkan uji lanjutan dengan Independent-Samples Kruskal-Wallis Test (α=0.05). Hasil penelitian menunjukkan terapi kombinasi kunyit madu mampu menurunkan jumlah sel radang secara nyata (P<0.05) sebesar 31.00±9.70 sel terhadap kontrol negatif. Terapi tunggal kunyit mampu meningkatkan jumlah sel fibroblas secara nyata (P<0.05) sebesar 63.20±22.60 sel terhadap kontrol negatif. Kesimpulan dari penelitian ini yaitu terapi kombinasi kunyit madu mampu menurunkan jumlah sel radang, sedangkan kunyit tunggal dapat meningkatkan jumlah sel fibroblas pasca gastrotomi.
English Abstract
Gastrotomy is a surgical procedure on the gastric organs by incising the major curvature to the lumen. Giving turmeric and honey therapy is known to have properties in the wound healing process. This study aims to determine the differences in the number of inflammatory cells and fibroblasts in the histopathological picture of the stomach of post-gastrotomy rats given honey turmeric therapy. This type of research is true experimental research. The research sample consisted of 25 male Sprague-Dawley rats aged 3 months with a weight of 400 grams. Rats were divided into 5 treatment groups with each group containing 5 rats. The treatment was given in the form of (K-) not given therapy, (K +) omeprazole 20 mg / KgBB, (P1) turmeric 500 mg / KgBB, (P2) honey 0.333 ml / ml, (P3) a combination of turmeric 250 mg / KgBB + honey 0 , 5 ml / ml PO once a day for 10 days. Gastric samples were processed using Hematoxylin-Eosin (HE) staining and observed using a 400x magnification microscope. Five field of view was taken and observed by using a purposive sampling. Quantitative data analysis used the Kruskal-Wallis test with a confidence level of 95% and continued with further tests with the Independent-Samples Kruskal-Wallis Test (α = 0.05). The results showed that honey turmeric combination therapy was able to significantly reduce the number of inflammatory cells (P <0.05) by 31.00 ± 9.70 cells against negative control. Turmeric single therapy was able to increase the number of fibroblast cells significantly (P <0.05) by 63.20 ± 22.60 cells against negative controls. This study concludes that honey turmeric combination therapy can reduce the number of inflammatory cells, while single turmeric can increase the number of fibroblast cells after gastrotomy.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0521130033 |
| Uncontrolled Keywords: | Kata Kunci: kunyit, madu, sel radang, sel fibroblas, gastrotomi. |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | yulia Chasanah |
| Date Deposited: | 24 Jan 2022 01:51 |
| Last Modified: | 11 Oct 2024 01:39 |
| URI: | http://repository.ub.ac.id/id/eprint/188674 |
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