Sholekhah, Salma Salas and drh. Widi Nugroho, Ph.D. and drh. Nurina Titisari, M.Si . (2021) Uji Sensitivitas Penggunaan Alat Pelos Sebagai Pemeriksaan Koprologi Fasciola sp. pada Sapi. Sarjana thesis, Universitas Brawijaya.
Abstract
Beberapa metode koprologi untuk deteksi Fasciola Sp. telah dikembangkan dengan tujuan meningkatkan sensitivitas uji. Namun metode - metode tersebut masih memerlukan waktu yang lama untuk persiapan sampel sebelum bisa di periksa dibawah mikroskop. Tujuan riset ini adalah untuk mengembangkan metode koprologi deteksi Fasciola Sp. yang sensitif, mudah dan cepat sehingga dapat meningkatkan efisiensi pengujian. Pengembangan metode didasarkan pada prinsip penyaringan ganda pada pori-pori 250 μm dan 45 μm. Bagian saringan 45 μm dimodifikasi berbentuk kerucut dengan bagian dasar yang rata dan dapat dilepas, memungkinkan pemeriksaan langsung telur yang mengendap di dasar alat dibawah mikroskop tanpa perlakuan tambahan. Pembuatan konsentrasi sampel telur Fasciola sp. didapatkan dengan cara menggerus beberapa cacing dewasa, menambahkan air hingga volume 10 mL, menyaring hasil gerusannya yang sudah ditambahkan feses pada saringan 250 μm, menampung filtratnya, dan melakukan pengenceran untuk mendapatkan konsentrasi suspensi 5 EPG, 15 EPG, 25 EPG dan 50 EPG. Pengujian sensitivitas alat Pelos dilakukan dengan mencampur konsentrasi suspensi 5 EPG, 15 EPG, 25 EPG dan 50 EPG dengan feses. Kemudian disaring menggunakan alat Pelos dan diberi pewarna methylene blue. Selanjutnya dilakukan pengamatan dibawah mikroskop perbesaran 4x dan 10x. Pengujian diulang sebanyak 20 kali per konsentrasi. Ukuran telur Fasciola sp. diukur menggunakan software ImageJ dan perhitungan sensitivitas menggunakan rumus a/a+c. Hasil dari penelitian ini didapatkan yakni pada konsentrasi 5 EPG memiliki sensitivitas 15 %, 15 EPG memiliki sensitivitas 75%, 25 EPG memiliki sensitivitas 100% dan 50 EPG memiliki sensitivitas 100%. Kesimpulan dari penelitian ini menunjukkan bahwa alat Pelos efektif untuk mendeteksi infeksi Fasciola sp. dari sedang hingga berat (25 EPG – 50 EPG). .
English Abstract
Some coprology methods for Fasciola Sp. detection has been developed with a purpose of increasing test sensitivity. However, those methods still need a long time for preparing the sample before it can be checked on a microscope. The purpose of this study is to develop more sensitive, easier, and faster for Fasciola Sp. detection coprology method in order to increase the test efficiency. The method development was based on the principle of double filtration at 250 micron and 45 micron pores. The design of 45 micron sieve has conical modified with flat base and can be removed to direct examination of eggs settling on the bottom of the device under the microscope without additional treatment. Sample concentration making of Fasciola sp. eggs were obtained by grinding some adult worms, adding water to a volume of 10mL, filtering the grind result added with fecal using 250 μm filtration, collecting the filtrate, and doing a dilution to get suspension concentration of 5 EPG, 15 EPG, 25 EPG, and 50 EPG. Sensitivity test on Pelos tool was done by mixing suspension concentration of 5 EPG, 15 EPG, 25 EPG, and 50 EPG with fecal. Then, it was filtered using Pelos tool and given a coloring of methylene blue. After that, the researcher did an observation under a microscope with 4x and 10x magnification. Tool testing was repeated 20 times per concentration. The size of Fasciolla sp. eggs was measured using ImageJ software and sensitivity calculation using a/a+c formula. The result of this research showed that on 5 EPG concentration, it has a sensitivity of 15%, 15 EPG has 75% sensitivity, 25 EPG has 100% sensitivity, and 50 EPG has also 100% sensitivity. In conclusion, this research shows that Pelos tools are effective to detect from average to heavy Fasciola sp. infection (25 EPG – 50 EPG) .
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0521130028 |
| Uncontrolled Keywords: | Kata kunci : Fasciolosis, Pelos, Fasciola sp. , feses sapi, penyaringan ganda, sapi, sensitivitas |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | yulia Chasanah |
| Date Deposited: | 21 Jan 2022 03:14 |
| Last Modified: | 25 Feb 2022 00:54 |
| URI: | http://repository.ub.ac.id/id/eprint/188616 |
![[thumbnail of DALAM MASA EMBARGO]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text (DALAM MASA EMBARGO)
- Salma Salas Sholekhah.pdf Restricted to Registered users only until 31 December 2023. Download (1MB) |
Actions (login required)
 |
View Item |