Ermasari, Anissa and Dr. Husnul Khotimah,, S.Si., M.Kes and Dr. dr. I Wayan Arsana Wiyasa,, SpOG(K) (2021) Pengaruh Ekstrak Kunyit (Curcuma longa) terhadap Kadar Superoksida Dismutase Otak dan Kadar Follicle Stimulating Hormone Serum Tikus Putih Betina (Rattus norvegicus) yang Dipapar Aluminium,. Magister thesis, Universitas Brawijaya.
Abstract
Paparan logam berat yang termasuk didalamnya aluminium merupakan salah satu faktor risiko terjadinya infertilitas melalui jalur stres oksidatif di otak. Aluminium akan mengganggu homeostasis besi (Fe) intraseluler dan terlibat dalam reaksi fenton yang akan meningkatkan Reactive Oxygen Species (ROS) serta menurunkan enzim antioksidan endogen, salah satu nya adalah superoksida Dismutase (SOD). Stres oksidatif yang meningkat menyebabkan terjadinya peroksidasi lipid sehingga terjadi gangguan pada gonadotropin releasing hormone (GnRH) pada hipotalamus, hal ini menyebabkan kegagalan hipofisis untuk melakukan sintesis dan sekresi Follicle Stimulating Hormone (FSH) & Luteinizing Hormone (LH). Kekurangan hormon FSH dapat menghambat proses folikulogenesis yang mengakibatkan infertilitas. Kurkumin adalah antioksidan yang terkandung dalam kunyit (Curcuma longa) dapat menghambat pembentukan ROS dan merangsang pembentukan enzim antioksidan endogen. Penelitian ini memiliki tujuan untuk mengetahui pengaruh ektrak kunyit pada kadar SOD otak dan kadar FSH serum tikus putih betina yang dipapar aluminium. Desain penelitian ini adalah post test only control group design, menggunakan sampel 25 ekor tikus yang terdiri dari 5 kelompok perlakuan yaitu kelompok kontrol negatif, kelompok kontrol positif (paparan aluminium 100 mg/KgBB), Kelompok perlakuan 1/P1 (ekstrak kunyit 100 mg/KgBB dan aluminium 100 mg/kgBB), Kelompok perlakuan 2/P2 (ekstrak kunyit 200 mg/KgBB dan aluminium 100 mg/kgBB), dan Kelompok perlakuan 3/P3 (ekstrak kunyit 400 mg/KgBB dan aluminium 100 mg/kgBB). Semua perlakuan diberikan peroral selama 30 hari dari hari ke-1 sampai hari ke-30. Pembedahan dilakukan pada saat fase estrus. Pemeriksaan kadar SOD otak dan FSH serum dengan menggunakan metode ELISA (Enzyme Linked Immunosorbent Assay) pada panjang gelombang 450nm. Analisis data menggunakan uji one way annova pada pada SPSS 26. Uji one way annova pada kadar SOD otak didapatkan p-value=0,0023 (α=0.05) sehingga terdapat perbedaan yang bermakna pada lima kelompok. Paparan aluminium dapat menurunkan kadar SOD secara signifikan dibandingkan dengan kelompok kontrol negatif. Akan tetapi, rerata kadar SOD pada P1, P2 dan P3 tidak ada perbedaan yang bermakna bila dibandingkan dengan kontrol positif. Uji one way annova pada kadar FSH serum didapatkan p-value=0,0018 (α=0.05). Paparan aluminium dapat menurunkan kadar FSH dan pemberian ekstrak kunyit dapat meningkatkan kadar FSH secara signifikan pada dosis 100 mg/KgBB. Paparan aluminium dapat menyebabkan Stres oksidatif di otak dan merupakan Endocrine Disrupting chemical (EDC) yang dapat menggangu sintesis hormon dan fungsi fisiologis reproduksi. Kurkumin dapat melindungi sel dari kerusakan oksidatif, menurunkan produksi ROS dan menetralkan radikal bebas serta secara tidak langsung dapat menginduksi ekspresi protein antioksidan seperti SOD, CAT dan GPx. Namun, Kurkumin memilki efek paradoks, yang dapat bertindak sebagai antioksidan dan prooksidan tergantung pada konsentrasi dan lingkungan seluler. Penurunan SOD pada P3 diduga karena efek paradoks tersebut. Kurkumin juga memiliki efek stimulasi padavii sistem reproduksi dengan memodulasi folikulogenesis, kunyit juga memiliki efek fitoestrogen yang dapat meningkatkan kadar estrogen dalam darah, diduga penurunan kadar FSH pada P3 disebabkan oleh efek umpan balik negatif oleh estrogen pada sekresi FSH. Kesimpulan dalam penelitian ini pemberian ekstrak kunyit tidak terbukti mencegah penurunan kadar SOD otak, tetapi ekstrak kunyit pada dosis 100 mg/KgBB dapat mencegah penurunan kadar FSH serum tikus putih betina (Rattus norvegicus) galur wistar yang dipapar aluminium
English Abstract
Exposure to heavy metals including aluminum is one of the risk factors for infertility through oxidative Stres pathways in the brain. Aluminum disrupts intracellular iron (Fe) homeostasis and participates in the Fenton reaction which results in an increase Reactive Oxygen Species (ROS) and decrease in endogenous antioxidant enzymes, including Superoxide Dismutase (SOD). Increased oxidative Stres promotes lipid peroxidation in the hypothalamus, which impairs the synthesis and secretion of gonadotropin releasing hormone (GnRH), causing the pituitary to fail to produce and secrete Follicle Stimulating Hormone (FSH) and Luteinizing Hormone LH. Infertility is caused by a lack of FSH hormone, which inhibits the process of folliculogenesis. Curcumin, an antioxidant found in turmeric (Curcuma longa), can decrease the development of reactive oxygen species (ROS) while also stimulating the production of endogenous antioxidant enzymes. The aim of this study to determine the effect of turmeric extract on brain SOD and FSH serum levels of female white rats exposed to aluminum. The study employed a post-test only control group design, with a sample of 25 rats divided into five treatment groups, including the negative control group (normal saline 1ml), Treatment groups 1/P1 (turmeric extract 100 mg/kgBW and aluminum 100 mg/kgBW), treatment 2/P2 (turmeric extract 200 mg/kgBW and aluminum 100 mg/kgBW), and treatment 3/P3 (turmeric extract 400 mg/kgBW and aluminum 100 mg/kgBW) were used. All treatments were given orally for 30 days. During the estrus phase, surgery is conducted. SOD and FSH serum levels in the brain were measured using the ELISA (Enzyme Linked Immunosorbent Assay) technique at 450nm. On SPSS 26, the one-way annova test was used to analyze the data. The p-value for a one-way annova test on brain SOD levels was 0.0023 (α=0.05), indicating that there were significant differences between the five groups. When compared to the negative control group, aluminum exposure can significantly lower SOD levels. However, when compared to the positive control, the mean SOD levels at P1, P2, and P3 were not substantially different. One way annova test on serum FSH levels obtained p-value = 0.0018<α (α=0.05). Aluminum exposure can reduce FSH levels and turmeric extract can significantly increase FSH levels at a dose of 100 mg/KgBW. Aluminum exposure can cause oxidative Stres in brain and it‘s an Endocrine Disrupting chemical (EDC) that can interfere with hormone synthesis and reproductive physiological functions. Curcumin can protect cells from oxidative damage, reduce ROS production and neutralize free radicals and can indirectly induce the expression of antioxidant proteins such as SOD, CAT and GPx. However, curcumin has a paradoxical effect, which can act as both an antioxidant and a pro-oxidant depending on the concentration and cellular environment. Decreasead level of brain SOD at P3 is thought to be due to this paradoxical effect. Curcumin also has a stimulatory effect on the reproductive system by modulating folliculogenesis, turmeric also has a phytoestrogen effect that can increase estrogen levels in the blood, it‘s suspected that the decrease in FSH levels at P3 is caused by a negative feedback effect by estrogen on FSH secretion.ix The conclusion in this study was that the administration of turmeric extract could not prevent a decrease in SOD levels, but turmeric extract at a dose of 100 mg/KgBB could prevent a decrease in serum FSH levels of female white rats (Rattus norvegicus) wistar strain exposed to aluminum
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 042106 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.2 Obstetrics |
| Divisions: | S2/S3 > Magister Kebidanan, Fakultas Kedokteran |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 03 Jan 2022 04:13 |
| Last Modified: | 24 Feb 2022 16:11 |
| URI: | http://repository.ub.ac.id/id/eprint/187744 |
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