Safira, Shania Cahya and Prof. Dr.dr. Sanarto Santoso, DTM&H, Sp.MK(K) and Prof. Dr. dr. Nurdiana, M.Kes (2021) Uji Efektivitas Ekstrak Etanol Biji Kopi Arabica (Coffea Arabica) Terhadap Jamur Candida Albicans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Jamur Candida albicans masih menjadi penyebab infeksi jamur terbanyak di Indonesia meupun di belahan dunia. Tingginya laju resistensi Candida albicans terhadap agen antifungal serta efek samping pengobatan bagi beberapa populasi menjadi ancaman kesehatan masyarakat akan semakin meningkatnya prevalensi infeksi Candida albicans. Diperlukan suatu inovasi agen antifungal yang mudah didapatkan serta melimpah keberadaannya. Tanaman Kopi (Coffea arabica) merupakan tanaman yang banyak dikultivasi di negara dengan penghasil kopi terbesar ke-3 di Dunia. Tanaman Kopi memiliki berbagai manfaat sejak zaman dahulu. Tanaman Kopi juga diketahui memiliki beberapa aktivitas antifungal walaupun masih sedikit literatur efek antifungal terhadap Candida spp. Penelitian ini menggunakan penelitian eksperimental laboratorium dengan desain true experimental. Fokus penelitian ditujukan pada keadaan koloni Candida albicans setelah dipaparkan ekstrak etanol Biji Kopi Arabika (Coffea arabica) secara in vitro melalui metode dilusi agar untuk menentukan pertumbuhan koloni pada tiap plate. Kelompok perlakuan pada penelitian ini dibagi menjadi 2 kelompok yaitu kelompok kultur Candida albicans dengan aquades (kontrol negatif) serta kelompok kultur Candida albicans dengan konsentrasi ekstrak 5%, 10%, 15%, 20%, dan 25%. Konsentrasi 5% telah menunjukkan adanya penurunan pertumbuhan koloni. Alasan itulah yang membuat penelitian menggunakan konsentrasi 5% ke atas. Hasil analisa statistik didapatkan nilai uji Kruskal Wallis sebesar 0.001 (p<0.05). Analisis data dilanjutkan menggunakan Uji Mann Whitney. Hasil analisis menunjukkan bahwa konsentrasi ekstrak Biji Kopi Arabika 0% atau kontrol negatif menghasilkan hasil yang berbeda signifikan terhadap konsentrasi 5%, 10%, 15%, 20%, dan 25%. Perbandingan antarkelompok lainnya menunjukkan nilai signifikansi kecuali konsentrasi 15% dengan 20% serta 20% dengan 25%. Uji signifikansi Spearman menghasilkan angka 0.000 dengan koefisien korelasi - 0.964. Hasil tersebut menunjukkan peningkatan konsentrasi ekstrak Biji Kopi Arabika akan menurunkan pertumbuhan koloni Candida albicans secara signifikan. Sehingga, dapat disimpulkan bahwa ekstrak Biji Kopi Arabika (Coffea arabica) memiliki efek antifungal terhadap jamur Candida albicans secara in vitro.
English Abstract
Candida albicans’ still the cause of the most fungal infections in Indonesia and in other parts of the world. The high rate resistance of Candida albicans to antifungal agents and side effects of treatment for some populations is a public health threatening for the increased prevalence of Candida albicans infection. An innovative antifungal agent that is easily available and abundant is needed. Arabika plant (Coffea arabica) is a plant that is widely cultivated in the country with the 3rd largest coffee producer in the world, Indonesia. Arabika plants have various benefits since ancient times. Arabika plants are also known to have some antifungal activity although there is still little literature on antifungal effects against Candida spp. This research used laboratory experimental research with true experimental design. The focus of the study was aimed at the condition of Candida albicans colonies after exposure to the ethanol extract of Arabica Coffee Bean (Coffea arabica) in vitro through the agar dilution method to determine colony growth on each plate. The treatment group in this study was divided into 2 groups, namely the Candida albicans culture group with distilled water (negative control) and the Candida albicans culture group with extract concentrations of 5%, 10%, 15%, 20%, and 25%. The 5% concentration has shown a decrease in colony growth. That is the reason why research uses a concentration of 5% and above. The results of statistical analysis showed that the Kruskal Wallis test value was 0.001 (p <0.05). Data analysis was continued using the Mann Whitney test. The results of the analysis showed that the concentration of 0% Arabica Coffee Bean extract or negative control produced significantly different results on the concentrations of 5%, 10%, 15%, 20%, and 25%. Other intergroup comparisons show a significance value except for concentrations of 15% with 20% and 20% with 25%. The Spearman significance test yields the number 0.000 with a correlation coefficient of -0.964. These results indicate that increasing the concentration of Arabica Coffee Bean extract will significantly reduce the growth of Candida albicans colonies. Thus, it can be concluded that the extract of Arabica Coffee Bean (Coffea arabica) has an in vitro antifungal effect against the Candida albicans.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0521060222 |
| Uncontrolled Keywords: | Coffea arabica, Candida albicans, antifungal, efek samping, Coffea arabica, Candida albicans, antifungal, side effects |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 28 Dec 2021 02:05 |
| Last Modified: | 23 Feb 2022 07:20 |
| URI: | http://repository.ub.ac.id/id/eprint/187667 |
![[thumbnail of DALAM MASA EMBARGO]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text (DALAM MASA EMBARGO)
Shania Cahya Safira.pdf Restricted to Registered users only until 31 December 2023. Download (14MB) |
Actions (login required)
 |
View Item |