Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice

W. Senoaji, - (2021) Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice. Biodiversitas, 22 (5). pp. 2750-2755. ISSN 2085-4722

Official URL: https://doi.org/10.13057/biodiv/d220537

Abstract

Pendekatan proteomik: Identifikasi protein vektor Nephotettix virescens yang menularkan virus tungro pada padi. Keanekaragaman hayati 22: 2750-2755. Sebagai vektor virus tungro, Nephotettix virescens merupakan salah satu hama utama pada kultivar padi. Associated Rice Tungro Spherical Virus menyediakan komponen pembantu untuk akuisisi Rice Tungro Bacilliform Virus yang menghasilkan gejala parah pada beras. Protein vektor yang dikodekan oleh virus menjadi transmisi virus tanaman yang kurang dipahami, terutama dalam mekanisme semi-persisten. Sangat penting untuk mengembangkan molekul baru untuk mengganggu mekanisme transmisi virus tanaman oleh vektor serangga. Penelitian ini bertujuan untuk mendeteksi protein pembantu pada vektor dengan proteomik. Pemisahan protein dilakukan menggunakan SDS-PAGE, kemudian diidentifikasi dengan Liquid Chromatography-Mass Spectrometry (LC-MS/MS) untuk menemukan kandidat protein. Hasil penelitian menunjukkan bahwa aktin teridentifikasi pada N. virescens sebagai protein yang bertanggung jawab terkait dengan penularan virus tungro ke dalam tanaman.

English Abstract

Proteomic approach: Identification of Nephotettix virescens vector protein transmitting the tungro virus in rice. Biodiversitas 22: 2750-2755. As a vector of tungro virus, Nephotettix virescens is one primary pest in rice cultivars. Associated Rice Tungro Spherical Virus provides a helper component for Rice Tungro Bacilliform Virus acquisition produced the severe symptoms in rice. The vector proteins encoded by the virus become a poorly understood transmission of plant viruses, particularly in semi-persistent mechanisms. It is essential to develop new molecules to interrupt the transmission mechanism of plant viruses by insect vectors. This study aimed to detect the helper proteins in vectors with proteomics. Protein separation was performed using SDS-PAGE, and then was identified by the Liquid Chromatography-Mass Spectrometry (LC-MS/MS) to find the candidate proteins. The result showed that actin was identified in N. virescens as responsible protein that related to transmitting tungro viruses into the plant.

Item Type:	Article
Uncontrolled Keywords:	Nephotettix virescens,protein, tungro, SDS-PAGE, MS/MS, actin
Depositing User:	Dra. Widia Permana., S.Sos., MAB.
Date Deposited:	16 Dec 2021 03:18
Last Modified:	10 Feb 2022 04:46
URI:	http://repository.ub.ac.id/id/eprint/187259

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