Uswah, Yolani Jamilatul and Dr. Ir. M. Fadjar,, M.Sc and Ir. Heny Suprastyani,, MS (2021) Pengaruh Infeksi Bakteri Aeromonas hydrophila Terhadap Kelulushidupan, Laju Pertumbuhan Spesifik dan Konversi Pakan Benih Ikan Lele (Clarias gariepinus) Strain MHC Generasi ke 3. Sarjana thesis, Universitas Brawijaya.
Abstract
Ikan lele dumbo (Clarias gariepinus) merupakan ikan perairan tawar yang banyak dibudidayakan di Indonesia. Meningkatnya permintaan pasar domestik dan peluang ekspor yang menjanjikan berdampak pada semakin meningkatnya minat usaha dan pengembangan teknologi budidaya intensif pada ikan ini (Prayogi, et al., 2016). Namun, budidaya ikan lele dumbo sering mendapat kendala, salah satunya adalah serangan bakteri patogen. A. hydrophila merupakan bakteri Gram negatif yang banyak ditemukan di perairan dan dapat menyerang ikan. Selain itu, A. hydrophila menyebaban penyakit Motile Aeromonas Septicemia (MAS) yang menyerang beberapa organ dalam seperti hati, limpa dan ginjal. Motile Aeromonas Septicemia (MAS) merupakan penyakit ikan sistemik. Salah satu solusi terhadap permasalahan tersebut adalah dengan melakukan perbaikan kualitas induk untuk menghasilkan benih ikan yang tahan patogen melalui seleksi marka molekuler pada gen Major Histocompatibility Complex (MHC). Oleh karena itu, perlu dilakukan identifikasi kinerja produksinya yaitu kelulushidupan, laju pertumbuhan spesifik dan konversi pakan pada ikan lele MHC generasi ketiga (F3). Penelitian ini bertujuan untuk mengetahui pengaruh infeksi bakteri A. hydrophila terhadap kelulushidupan, laju pertumbuhan spesifik dan konversi pakan benih ikan lele (C. gariepinus) strain MHC Generasi ke 3. Metode yang digunakan pada penelitian ini yaitu metode eksperimen, dengan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 6 perlakuan, 2 kontrol dan 3 kali ulangan. Perlakuan yang digunakan yaitu benih ikan yang diinfeksi bakteri A. hydrophila dengan kepadatan 106, 107, dan 108. Sedangkan kontrol meliputi ikan lele normal dan ikan lele MHC tanpa diinfeksi A. hydrophila. Kemudian diamati selama 2 minggu dilakukan perhitungan terhadap parameter utama yaitu kelulushidupan, laju pertumbuhan spesifik dan konversi pakan pada hari ke-0, 7, dan 14 pengamatan dan parameter penunjang yaitu suhu, pH, DO, ammonia, nitrit dan nitrat. Hasil penelitian menunjukan bahwa benih ikan lele strain MHC generasi ke 3 memberikan perfoma yang lebih baik dilihat dari kelulushidupan, laju pertumbuhan spesifik dan konversi pakannya ketika diinfeksi menggunakan bakteri A. hydrophila. Konsentrasi dari hasil uji LD50 yaitu 2,17×108. Rata rata kelulushidupan benih ikan lele MHC generasi ke 3 sebesar 79%, laju pertumbuhan spesifik 4,30%/hari, dan konversi pakan 1,11, sedangkan rata rata kelulushidupan benih ikan lele non-MHC sebesar 24%, laju pertumbuhan spesifik 2,30%/hari, dan konversi pakan 1,12.
English Abstract
African catfish (Clarias gariepinus) is a freshwater fish that is widely cultivated in Indonesia. The increasing domestic market demand and promising export opportunities have an impact on the increasing business interest and the development of intensive cultivation technology for this fish (Prayogi, et al., 2016). However, African catfish cultivation often faces obstacles, one of which is the attack of pathogenic bacteria. A. hydrophila is a gram-negative bacterium that is found in many fresh waters and can attack fish. In addition, A. hydrophila causes Motile Aeromonas Septicemia (MAS) disease which attacks several internal organs such as the liver, spleen, and kidneys. Motile Aeromonas Septicemia (MAS) is a systemic fish disease. One solution to this problem is to improve the quality of the broodstock to produce pathogen-resistant fish seeds by selecting molecular markers on the Major Histocompatibility Complex (MHC) gene. Therefore, it is necessary to identify the production performance, survival rate, specific growth rate, and feed conversion ratio in third-generation MHC catfish (F3). This study aims to determine the effect of A. hydrophila bacterial infection on survival rate, specific growth rate, and feed conversion ratio of 3rd generation MHC strain catfish (C. gariepinus). The method used in this study is the experimental method, with a factorial completely randomized design (CRD) analysis consisting of 6 treatments, 2 controls, and 3 replications. The treatments used were fish fingerlings that were infected with A. hydrophila bacteria with consentration of 106, 107, and 108. While the controls included normal catfish and MHC catfish without being infected by A. hydrophila. Then observed for 2 weeks the main parameters were calculated, survival rate, specific growth rate, and feed conversion ratio on days 0, 7, and 14 observations and supporting parameters, temperature, pH, DO, ammonia, nitrite, and nitrate. The results showed that the 3rd generation MHC strain catfish fingerlings gave a better performance in terms of survival rate, specific growth rate, and feed conversion ratio when infected using A. hydrophila bacteria. The concentration of the LD50 test results is 2.17 × 108. The average survival rate of MHC catfish fingerlings 3rd generation was 79%, the specific growth rate was 4.30% / day, and feed conversion ratio 1.11, while the non-MHC catfish fry survival rate was 24%, the specific growth rate was 2, 30% / day, and feed conversion ratio 1.12.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0521080100 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Budidaya Perairan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 30 Nov 2021 03:47 |
| Last Modified: | 24 Feb 2022 07:07 |
| URI: | http://repository.ub.ac.id/id/eprint/186854 |
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