Salsabila, Dhea (2021) Studi Antibodi Poliklonal Sebagai Kandidat Kit Deteksi African Swine Fever Berbasis Protein Rekombinan p22-ASFV Pada Hewan Coba Kelinci (Oryctolagus cuniculus). Sarjana thesis, Universitas Brawijaya.
Abstract
African swine fever merupakan penyakit yang disebabkan oleh African swine fever virus (ASFV) yang memiliki tingkat kematian yang tinggi pada babi. ASF menjadi ancaman global karena kurangnya vaksin saat ini dan tidak tersedianya pengobatan untuk penyakit yang memiliki angka morbiditas dan fatalitas kasus hingga 100% ketika strain patogenik ASFV terlibat. Tujuan dari penelitian ini adalah untuk mempelajari karakter poliklonal antibody hasil induksi protein spesifik p22-ASFV fragmen yang dikloning dan diekspresikan pada media kultur sel bakteri E.coli BL21 pada kelinci jantan New Zealand White (Oryctolagus cuniculus). Sampel protein rekombinan p22-ASFV yang digunakan berasal dari PUSVETMA Surabaya. Lalu dilakukan purifikasi protein rekombinan p22 dengan metode elektroforesis SDS-PAGE dan dilanjutykan metode elektroelusi terhadap protein dengan berat molekul 22 kDa. Imunisasi pada kelinci jantan New Zealand White (Oryctolagus cuniculus) diberikan secara subkutan 800µl yang ditambah adjuvant CFA (Complete Freund’s Adjuvant) dan IFA (Incomplate Freund’s Adjuvant). Metode indirect ELISA dilakukan untuk mengukur titer antibodi dan melihat karakteristik antibodi dilakukan dengan metode Western Blot. Uji imunogenisitas protein p22 menggunakan metode indirect ELISA memiliki nilai titer antibodi tertinggi pada bleeding minggu ke-7 dengan nilai . Pada uji Western Blot menunjukkan bahwa antibodi anti-p22 dapat mengenali antigen p22 pada berat molekul 21,93 kDa.
English Abstract
African swine fever is a disease caused by African swine fever virus (ASFV) which has a high mortality rate in pigs. ASF is a global threat due to the current lack of vaccines and the unavailability of treatment for diseases that have a morbidity and case fatality rate of up to 100% when pathogenic ASFV strains are involved. The purpose of this research is to study the character of polyclonal antibody specific results of the induction of p22-ASFV protein fragments were cloned and expressed on the cell culture media E.coli BL21 on male New Zealand White rabbits (Oryctolagus cuniculus). Samples P22-ASFV recombinant protein is derived from PUSVETMA Surabaya. Purification of the recombinant P22 protein was carried out using the SDS-PAGE electrophoresis method and continued with the electroelution method for proteins with a molecular weight of 22 kDa. Immunization of male New Zealand White rabbits (Oryctolagus cuniculus) was administered subcutaneously 800 µL then added with adjuvant CFA (Complete Freund's Adjuvant) and IFA (Incomplete Freund's Adjuvant). The Indirect ELISA method was used to measure the antibody titers and characteristics of antibodies was conducted by Western Blot method. The immunogenicity test for p22 protein using the Indirect ELISA method had the highest antibody titer values in bleeding week 7 with a value of 1.144. The Western Blot test showed that the anti-p22 antibody could recognize the p22 antigen at a molecular weight of 21,93 kDa
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 636.089 |
| Uncontrolled Keywords: | ASF, Protein rekombinan p22, Antibodi--Recombinant protein p22, Antibodies. |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Unnamed user with email gaby |
| Date Deposited: | 25 Oct 2021 08:34 |
| Last Modified: | 23 Feb 2022 07:40 |
| URI: | http://repository.ub.ac.id/id/eprint/185990 |
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Text (DALAM MASA EMBARGO)
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