Asiyah, Nurul (2021) Uji Efektifitas Ekstrak Etanol Buah Pare (Momordica charantia L) sebagai Antimikroba terhadap Pertumbuhan Bakteri Klebsiella pneumoniae Strain ESBL secara in vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Indonesia merupakan negara tropis di mana masih banyak ditemukan penyakit infeksi. Infeksi dapat disebabkan oleh bakteri, virus, parasit, dan jamur. Klebsiella pneumoniae adalah bakteri penyebab infeksi yang meliputi infeksi nosokomial pada orang dewasa dan anak-anak, infeksi saluran pernafasan dan ISK. Bakteri ini merupakan patogen oportunistik yang menyerang pasien dengan sistem imunitas menurun maupun yang dirawat di ruang ICU. Klebsiella pneumoniae penghasil Extended Spectrum β-lactamase (ESBL) menunjukkan resistensi terhadap beberapa antibiotik. Enzim ESBL memberi sifat resisten terhadap hampir semua antibiotik β-lactamase kecuali golongan carbapenem dan cephamycin, sehingga perlu dikembangkan alternatif terapi lain. Tanaman pare dikenal masyarakat sebagai tanaman sayur tetapi mempunyai khasiat Kesehatan bagi manusia. Buah pare (Momordica charantia L) mengandung beberapa senyawa aktif bersifat sebagai antimikroba. Senyawa tersebut ialah senyawa flavonoid, alkaloid, dan saponin. Penelitian ini bertujuan untuk mengetahui efek antimikroba ekstrak etanol buah pare (Momordica charantia L.) terhadap pertumbuhan Klebsiella pneumonaie (ESBL) secara in vitro. Desain penelitian menggunakan true experimental laboratorium dengan metode difusi sumuran. Proses ekstraksi buah pare menggunakan metode maserasi dengan pelarut etanol 96% dan konsentrasi ekstrak yang digunakan meliputi 25% 25%, 30%, 35%, 40%, 45%, dan 50% dengan empat kali pengulangan. Selain itu digunakan kontrol positif yaitu antibiotik Meropenem dan kontrol negatif berisi aquades. Hasil penelitian menunjukkan pada konsentrasi 25%, 30%, 35%, 40%, 45%, dan 50% terbentuk zona inhibisi berturut-turut sebesar 8.10mm, 8.13mm, 8.55mm, 8.57mm, 9.11mm, 9.13mm. Uji normalitas dan homogenitas menunjukkan data tidak normal dan tidak homogen, sehingga dilanjutkan dengan uji Kruskall Wallis. uji Kruskall Wallis menunjukkan bahwa pemberian ekstrak etanol buah pare berpengaruh secara signifikan terhadap pertumbuhan Klebsiella pneumoniae strain ESBL (sig=0,000; p<0,05). Sedangkan uji korelasi Spearman menunjukkan adanya hubungan sangat kuat dan semakin tinggi konsentrasi akan membentuk diameter zona hambat yang semakin besar (koefisien= 0,961). Kesimpulannya, ekstrak etanol buah pare mempunyai efek antimikroba terhadap Klebsiella pneumoniae (ESBL) secara in vitro dengan metode difusi sumuran.
English Abstract
Indonesia is a tropical country where many infectious diseases are still found. Infections can be caused by bacteria, viruses, parasites, and fungi. Klebsiella pneumoniae is a bacterial cause of infections that include nosocomial infections in adults and children, respiratory tract infections and UTIs. This bacterium is an opportunistic pathogen that attacks patients with decreased immune systems and those who are treated in the ICU. Klebsiella pneumoniae producing Extended Spectrum -lactamase (ESBL) shows resistance to several antibiotics. ESBL enzymes are resistant to almost all -lactamase antibiotics except for the carbapenem and cephamycin groups, so it is necessary to develop other therapeutic alternatives. Pare plants are known to the public as vegetable plants but have health benefits for humans. Bitter gourd (Momordica charantia L) contains several active compounds that act as antimicrobials. These compounds are flavonoids, alkaloids, and saponins. This study aimed to determine the antimicrobial effect of the ethanol extract of bitter melon (Momordica charantia L.) on the growth of Klebsiella pneumonaie (ESBL) in vitro. The research design used a true experimental laboratory with well diffusion method. The extraction process of bitter melon uses maceration method with 96% ethanol solvent and the extract concentration used includes 25% 25%, 30%, 35%, 40%, 45%, and 50% with four repetitions. In addition, a positive control was used, namely Meropenem antibiotics and a negative control containing aquades. The results showed that at concentrations of 25%, 30%, 35%, 40%, 45%, and 50%, inhibition zones of 8.10mm, 8.13mm, 8.55mm, 8.57mm, 9.11mm, 9.13mm were formed, respectively. The normality and homogeneity test showed that the data was not normal and not homogeneous, so it was continued with the Kruskall Wallis test. Kruskall Wallis test showed that administration of ethanol extract of bitter melon had a significant effect on the growth of Klebsiella pneumoniae strain ESBL (sig=0,000; p<0,05). While the Spearman correlation test showed a very strong relationship and the higher the concentration, the larger the diameter of the inhibition zone (coefficient = 0.961). In conclusion, the ethanolic extract of bitter melon has an antimicrobial effect against Klebsiella pneumoniae (ESBL) in vitro by well diffusion method.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 610 |
| Uncontrolled Keywords: | Klebsiella pneumoniae, Extended Spectrum β-lactamase (ESBL), Flavonoid, saponin, alkaloid, efek antimikroba, Pare (Momordica charantia), Klebsiella pneumoniae, Extended Spectrum β-lactamase (ESBL), Flavonoids, saponins, alkaloids, antimicrobial effect, Pare (Momordica charantia L). |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Unnamed user with email gaby |
| Date Deposited: | 24 Oct 2021 05:27 |
| Last Modified: | 25 Feb 2022 06:30 |
| URI: | http://repository.ub.ac.id/id/eprint/185752 |
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