Ramadhan, Ahmad and Dian Handayani, S.KM., M.Kes., PhD. and Inggita Kusumastuty, S.Gz., M.Biomed. (2020) Pengaruh Pemberian Beras Coklat terhadap Kadar Serum Nitric Oxide pada Tikus Putih yang Diberi Diet High Fat High Fructose Sebagai Hewan Coba Model Obesitas. Sarjana thesis, Universitas Brawijaya.
Abstract
Prevalensi obesitas terus mengalami peningkatan di Indonesia. Obesitas dapat meningkatan risiko penyakit tidak menular, seperti diabetes mellitus, penyakit jantung koroner, dan hipertensi. Nitric Oxide (NO) memiliki peran beragam di tubuh, salah satunya pengaturan tekanan darah. Beras coklat memiliki berbagai kandungan antioksidan, salah satunya asam ferulat yang berpotensi sebagai antioksidan. Tujuan penelitian ini untuk mengetahui pengaruh pemberian beras coklat terhadap kadar serum NO pada tikus putih yang diberi diet high fat high fructose sebagai hewan coba model obesitas. Subjek penelitian ini adalah tikus putih galur Sprague Dawley obesitas (n=35) terbagi menjadi 3 kelompok perlakuan beras coklat dengan dosis 12,43%, 24,86%, 37,29% dari berat pakan dan 2 kelompok kontrol. Setelah induksi obesitas dengan diet tinggi lemak dan cairan fruktosa 30% selama 14 minggu dan intervensi beras coklat selama 8 minggu tikus dibedah dan diambil serumnya. Pengukuran NO menggunakan metode tidak langsung dengan menghitung metabolit NO (NOx) menggunakan reaksi Griess lalu dibaca dengan microplate reader 540 nm. Hasil penelitian menunjukkan kadar NOx pada kelompok normal, HFHF, HFHF+BR I, HFHF+BR II, dan HFHF+BR III secara berturut-turut 38,82; 48,77; 51,83; 51,38; 43,59 μM. Uji statisitik One way ANOVA menunjukkan tidak ada perbedaan kadar NOx yang signifikan antarkelompok perlakuan (p=0,816). Walaupun demikian, terdapat tren penurunan pada kelompok intervensi. Kesimpulan penelitian ini adalah pemberian beras coklat mampu menurunkan kadar NOx.
English Abstract
The prevalence of obesity continues to increase in Indonesia. Obesity can increase the risk of non-communicable diseases, such as diabetes mellitus, coronary heart disease, and hypertension. Nitric Oxide (NO) has a variety of roles in the body, one of which is blood pressure regulation. Brown rice has various antioxidant contents, one of which is ferulic acid which has the potential as an antioxidant. The purpose of this study was to determine the effect of administration brown rice to serum NO levels in white rats fed a high fat high fructose diet as an experimental animal model of obesity. The subjects of this study were obese Sprague Dawley strain rats (n = 35) divided into 3 treatment groups of brown rice with 12.43%, 24.86%, 37.29% of feed weight and 2 control groups. After the induction of obesity with a diet high in fat and 30% fructose fluid for 14 weeks and brown rice intervention for 8 weeks the rats were dissected and their serum taken. NO measurements using the indirect method by calculating NO metabolites (NOx) using the Griess reaction then read by 540 nm microplate reader. The results showed NOx levels in the normal group, HFHF, HFHF+BR I, HFHF+BR II, and HFHF+BR III 38.82; 48.77; 51.83; 51.38; 43.59 μM respectively. One way ANOVA static test showed no significant difference in NOx levels between treatment groups (p=0.816). However, there is a downward trend in the intervention group. The conclusion of this study is administration of brown rice can reduce levels of NOx
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520060005 |
| Uncontrolled Keywords: | Obesitas, Nitric Oxide, Beras Coklat, Obesity, Nitric Oxide, Brown Rice |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) > 615.107 24 Experimental research |
| Divisions: | Fakultas Kedokteran > Ilmu Gizi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 18 Feb 2021 10:17 |
| Last Modified: | 15 Sep 2022 02:49 |
| URI: | http://repository.ub.ac.id/id/eprint/183315 |
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