Ramadhania, Frida (2020) Penentuan Parameter Kinetik, Efisiensi Penggunaan Berulang, dan Kestabilan Enzim Xilanase dari Aspergillus niger yang Diamobilkan pada Matriks Pasir Laut Teraktivasi Asam. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase merupakan kelompok enzim ekstraseluler yang dapat menghidrolisis xilan menjadi xilosa. Xilanase diperoleh melalui proses isolasi dari beberapa jenis mikroorganisme, salah satunya Aspergillus niger. Amobilisasi enzim dilakukan untuk mencegah kerusakan enzim akibat pengaruh lingkungan. Pada penelitian ini digunakan matriks pasir laut untuk amobilisasi dengan metode adsorpsi. Penelitian ini bertujuan untuk mengetahui nilai parameter kinetik (Vmaks dan KM), efisiensi penggunaan berulang, dan kestabilan enzim xilanase yang diamobilkan pada matriks pasir laut teraktivasi asam. Parameter kinetik ditentukan dengan mengukur aktivitas xilanase pada variasi konsentrasi (0,1; 0,5; 1; 1,5; 2) %. Kestabilan enzim xilanase bebas ditentukan dengan mengukur aktivitas xilanase pada suhu (4, 30, 55) oC dengan variasi lama penyimpanan (0, 1, 2, 3) hari, sedangkan xilanase amobil pada suhu (4, 30, 60) oC dengan variasi lama penyimpanan (0, 1, 2, 3, 4, 5, 6, 7) hari. Pada penelitian ini dihasilkan nilai paremeter kinetik enzim xilanase bebas yaitu Vmaks sebesar 16,155 μg/mg.menit dan KM sebesar 0,16% sedangkan pada enzim xilanase amobil nilai Vmaks sebesar 32,68 μg/mL.menit dan KM sebesar 0,22%. Efisiensi penggunaan berulang enzim xilanase amobil dapat digunakan hingga 3 kali dengan persentaase efisiensi sebesar 49,82%. Enzim xilanase bebas stabil pada suhu 40C hingga lama penyimpanan satu hari dengan persentase aktivitas enzim sebesar 38,26%, sedangkan enzim xilanase amobil stabil pada suhu 40C hingga penyimpanan 4 hari aktivitas enzim sebesar 67,52%.
English Abstract
Xylanase is a group of extracellular enzymes that can hydrolyze xylan to xylose. Xylanase can be obtained by isolation process from several types of microorganisms, which one is Aspergillus niger. Enzyme immobilization is carried out to prevent enzyme damage due to environmental influences. In this research the used sea sand matrix for immobilization by adsorption methods. This research aims to determine the value of kinetic parameters (Vmax and KM), the efficiency of repeated use, and the stability of the xylanase enzyme that is immobilized on an acidic activated sea sand matrix. Kinetic parameters were determined by measured xylanase activity at various concentrations (0.1; 0.5; 1; 1.5; 2) %. The stability of the xylanase enzyme is determined temperature at (4, 30, 55) oC and storage time variations were (0, 1, 2, 3) days, while immobilized xylanase temperature at (4, 30, 60) oC with storage time variations were (0, 1, 2, 3, 4, 5, 6, 7) days. The result are shows that the value of kinetic parameters of free xylanase enzymes were obtained Vmax was 16.155 μg / mg.minute and KM was 0.16%, while in immobilized xylanase enzymes the value of Vmax was 32.68 μg / mL.minute and KM was 0.22%.The efficient of repeated use of the immobilized xylanase enzyme can be used up to 3 times with efficiency at 49.82%. The free xylanase enzyme was stable at 40C until one day storage time with the percentage of enzyme activity was 38.26%, while the immobilized xylanase enzyme was stable at 40C until 4 days of enzyme activity was 67.52%.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520090076 |
| Uncontrolled Keywords: | Xilanase, Aspergillus niger, Pasir Laut, Kinetik, Kestabilan |
| Subjects: | 500 Natural sciences and mathematics > 541 Physical chemistry > 541.3 Miscellaneous topics in physical chemistry > 541.33 Surface chemistry > 541.335 Adsorption |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | ismanto |
| Date Deposited: | 04 Feb 2021 06:46 |
| Last Modified: | 12 Jul 2022 01:25 |
| URI: | http://repository.ub.ac.id/id/eprint/182438 |
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