-, Yuliati (2020) Pengaruh Konsentrasi Auksin dan Sitokinin terhadap pertumbuhan Planlet Krisan ( Chrysanthenum sp). Sarjana thesis, Universitas Brawijaya.
Abstract
Tanaman krisan (Chrysanthemum sp) adalah salah satu tanaman hias dari Family Asteraceae (Dirjen Hortikultura, 2010) yang memiliki banyak manfaat. Produksi bunga krisan cenderung mengalami penurunan, hal tersebut disebabkan karena petani bunga menggunakan bibit yang tidak berkualitas sehingga pertumbuhan tanaman tidak optimal. Soetopo et al. (2013) menyatakan bahwa peningkatan produksi tanaman krisan tergantung pada kualitas bibit yang digunakan. Secara umum perbanyakan bunga krisan dilakukan dengan dua cara yaitu dengan cara konvensional dan moderen. Dalam kultur jaringan, organogenesis dalam pembentukan dan perkembangan tunas, akar serta pembentukan kalus dipicu oleh Zat Pengatur Tumbuh (ZPT), yaitu sitokinin dan auksin. Perbedaan ZPT yang diberikan pada media kultur akan memberikan pengaruh yang berbeda pada eksplan yang ditanam. (Thengane et al., 2006). Auksin tidak berfungsi dengan baik bila tidak berinteraksi dengan ZPT lainya seperti Sitokinin maupun Nutrisi lainya, namun demikian sering kali dibutuhkan keduanya tergantung pada perbandingan dan rasio Auksin terhadap Sitokinin maupun sebaliknya. Tujuan dari penelitian ini adalah untuk mendapatkan kombinasi konsentrasi sitokinin dan auksin yang tepat pada pertumbuhan eksplan Krisan. Hipotesis yang diajukan dalam penelitian ini adalah, pemberian Auksin dan sitokinin dengan konsentrasi tertentu dapat menstimulir pertumbuhan terbaik pada Planlet Krisan. Penelitian ini dilaksanakaan di UPT Kultur Jaringan Gedung Budidaya Pertanian, Fakultas Pertanian Universitas Brawijaya pada bulan Maret 2019 - Mei 2019. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 9 kombinasi sitokinin (BAP) dan auksin (NAA). Perlakuan tersebut yaitu A1 (BAP 1,30 ppm + NAA 0,20 ppm), A2 (BAP 1,50 ppm + NAA 0,20 ppm), A3 (BAP 1,70 ppm + NAA 0,20 ppm), A4 (BAP 1,30 ppm + NAA 0,35 ppm), A5 (BAP 1,50 ppm + NAA 0,35 ppm), A6 (BAP 1,70 ppm + NAA 0,35 ppm, A6 ( BAP 1,70 ppm + NAA 0,35 ppm, A7 ( BAP 1,30 ppm + NAA 0,50 ppm) A8 ( BAP 1,50 ppm + NAA 0,50 ppm) dan A9 ( BAP 1,70 ppm +NAA 0,50 ppm). Setiap perlakuan diulang sebanyak 3 kali sehingga jumlah keseluruhan 27 botol kultur, setiap botol terdiri dari 2 eksplan sehingga terdapat 54 eksplan. Pengamatan dilakukan setiap minggunya. Parameter pengamatan meliputi jumlah tunas, jumlah daun, jumlah akar, tinggi eksplan, persentase eksplan hidup, mati dan kontaminasi. Hasil pengamatan diuji dengan uji perbandingan BNT (Beda Nyata Terkecil) dengan taraf kesalahan 5% Hasil penelitian menunjukan bahwa kombinasi BAP + NAA pada minggu ke 6, minggu ke 7 dan minggu ke 8 setelah tanam memberikan pengaruh nyata pada parameter jumlah tunas. Perlakuan BAP + NAA memberikan pengaruh tidak nyata pada parameter jumlah daun dan parameter tinggi eksplan di semua perlakuan, sedangkan pertumbuhan akar pada eksplan krisan sangat lambat sehingga di amati pada akhir pengamatan minggu ke -8 setelah tanam, didapat hasil bahwa 100% akar belum tumbuh. Parameter eksplan hidup menunjukan bahwa perlakuan terbaik A3, A5, A7, A8, A9 dengan persentase eksplan hidup 100%, sedangkan persentase eksplan yang mati sebesar 0 % pada semua perlakuan. persentase ekplan yang terkontaminasi 0 % pada perlakuan A3, A5, A7, A8, dan A9.
English Abstract
Chrysanthemum sp is one of the ornamental plants from Asteraceae Family (Dirjen Hortikultura, 2010) which has many benefits. The production of it tends to decline, it is due to the farmers that use the poor quality seeds which leads to unideal growth of the plant. Soetopo et al. (2013) state that the production of the Chrysanthemum sp plants raise in accordance with the good quality of the seeds. In general, there are two ways to multiple the Chrysanthemum sp which are conventionally and modernly. In tissue culture, the organogenesis in the formation and growth of the shoots, roots as well as callus are triggered by the growing regulatory substances (ZPT), namely cytokine and auxin. The different ZPT treatment to the culture media the different effect on the planted explants (Thengane et al., 2006). The auxin does not function properly if it does not interact with other ZPT such as cytokine or other nutrients, but both of them are often needed depend on the comparison and ratio of the Auxin to Cytokine and vice versa. The purpose of this research is to obtain a combination of the best concentration of cytokine and auxin on the growth of Chrysanthemum sp explants. The hypothesis proposed in this research is that the treatment of auxin and cytokines with certain concentrations can stimulate the best growth on the Chrysanthemum sp Plantlet. This research was conducted at UPT Tissue Culture Aquaculture Building, Faculty of Agriculture at Brawijaya University in March 2019 - May 2019. This research used the complete random draft (RAL) method with 9 combinations of cytokines (BAP) and auxin (NAA). The treatments were A1 (BAP 1,30 ppm + NAA 0,20 ppm), A2 (BAP 1,50 ppm + NAA 0,20 ppm), A3 (BAP 1,70 ppm + NAA 0,20 ppm), A4 (BAP 1,30 ppm + NAA 0,35 ppm), A5 (BAP 1,50 ppm + NAA 0,35 ppm), A6 (BAP 1,70 ppm + NAA 0,35 ppm, A6 ( BAP 1,70 ppm + NAA 0,35 ppm, A7 ( BAP 1,30 ppm + NAA 0,50 ppm) A8 ( BAP 1,50 ppm + NAA 0,50 ppm) and A9 ( BAP 1,70 ppm +NAA 0,50 ppm). Each treatment was repeated 3 times so that there were 27 culture bottles, each bottle consists of 2 explants so that there were 54 explants. The observation was done every week. The observation parameters included the number of shoots, the number of leaves, the number of roots, time, height of explant, the percentage of live, death and contaminated explants. The observation results were tested with BNT comparison test (smallest real difference) with 5% error status. The results showed that the combination of BAP + NAA in Week 6, Week 7 and week 8 after planting had a tangible effect on the number of shoots parameters. The BAP + NAA treatment gave no significant effect on the leaves number parameter and the high parameter of the explants in all treatments, while the growth of roots on the Chrysanthemum sp explants were very slow so that it were observed at the end of the 8th week after planting, the results were 100% of roots have not grown. The live explant parameters showed that the best treatment were the A3, A5, A7, A8, A9 with 100% percentage of live explants, meanwhile the percentage of died explants were 0% in all treatments. Moreover, the percentage of contaminated explants were 0% at the treatment A3, A5, A7, A8, and A9.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2020/69/052003723 |
| Uncontrolled Keywords: | - |
| Subjects: | 600 Technology (Applied sciences) > 635 Garden crops (Horticulture) > 635.9 Flowers and ornamental plants |
| Divisions: | Fakultas Pertanian > Budidaya Pertanian |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 24 Aug 2020 07:34 |
| Last Modified: | 11 Apr 2023 02:26 |
| URI: | http://repository.ub.ac.id/id/eprint/181131 |
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