Pradibdo, Ruth Clarita (2019) Pengaruh Pemberian Ekstrak Daun Moringa Oleifera terhadap Penurunan Kadar Alanin Aminotransferase (ALT) dan Aspartat Aminotransferase (AST) Serum Tikus Model Fibrosis Hati akibat Injeksi Kronis Karbon Tetraklorida (CCl4). Sarjana thesis, Universitas Brawijaya.
Abstract
Secara global, penyakit hati kronis merupakan masalah kesehatan yang cukup banyak ditemui sehingga sangat penting dilakukan penelitian-penelitian terkait usaha penatalaksanaannya. Alanin aminotransferase (ALT) dan aspartat aminotransferase (AST) merupakan enzim yang dapat meningkat dalam plasma akibat injuri sel hepar (hepatosit). Diketahui bahwa senyawa seperti karbon tetraklorida (CCl4) dapat memicu injuri hepatosit melalui pembentukan radikal bebas. Radikal bebas sendiri dapat dieliminasi dengan antioksidan. Daun Moringa oleifera mengandung antioksidan yang bersifat hepatoprotektif dengan menangkal radikal bebas dan injuri hepatosit. Penelitian dengan metode randomized posttest only controlled group design ini bertujuan mengetahui pengaruh pemberian Moringa oleifera (MO) terhadap ALT dan AST serum tikus model yang diberikan injeksi kronis CCl4. Penelitian menggunakan 5 kelompok tikus berbeda. Kadar ALT dan AST serum diperiksa dengan metode Optimized UV-test berdasarkan metode modifikasi IFCC (International Federation of Clinical Chemistry) tanpa pyridoxal phosphate. Hasil uji Mann Whitney U menunjukkan pemberian MO menghasilkan kadar ALT yang lebih rendah, tidak signifikan pada dosis 150 mg/kgBB (p=0.917) dan signifikan pada dosis 600 mg/kgBB (p=0.009), dibandingkan kelompok tanpa pemberian MO. Sedangkan AST ditemukan tidak terpengaruh secara signifikan dengan pemberian MO, baik dosis 150 mg/kgBB (p=0.076) maupun dosis 600 mg/kgBB (p=0.117). Dapat disimpulkan bahwa pemberian MO berpengaruh terhadap penurunan kadar ALT serum dan tidak berpengaruh terhadap kadar AST serum tikus model fibrosis hati akibat injeksi kronis CCl4.
English Abstract
Globally, chronic liver disease is a fairly common health problem that is very important to do research related to its management efforts. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) are enzymes that can be increased in plasma due to liver cell (hepatocytes) injury. It is known that compounds such as carbon tetrachloride (CCl4) can trigger hepatocyte injury through the formation of free radicals. Free radicals themselves can be eliminated by antioxidants. Moringa oleifera leaves contain antioxidants that are hepatoprotective by counteracting free radicals and injury to hepatocytes. This study using randomized posttest only controlled group design aims to determine the effect of Moringa oleifera (MO) administration on ALT and AST serum in rats with CCl4-induced liver fibrosis. The study used 5 different groups of mice. Serum ALT and AST levels were examined by the Optimized UV-test method based on the IFCC (International Federation of Clinical Chemistry) modification method without pyridoxal phosphate. The Mann Whitney U test results showed that the administration of MO resulted in lower ALT levels, not significantly at a dose of 150 mg / kgBB (p = 0.917) and significant at a dose of 600 mg / kgBB (p = 0.009), compared to the group without MO administration. Whereas AST was found not significantly affected by the administration of MO, both a dose of 150 mg / kgBB (p = 0.076) and a dose of 600 mg / kgBB (p = 0.117). It can be concluded that the administration of MO affects the decrease in serum ALT levels and does not affect the serum AST levels of rat liver fibrosis models due to chronic CCl4 injection.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/608/052001463 |
| Uncontrolled Keywords: | Moringa oleifera, ALT, AST, CCl4-Moringa oleifera, ALT, AST, CCl4. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 05 Aug 2020 08:13 |
| Last Modified: | 22 Oct 2021 03:21 |
| URI: | http://repository.ub.ac.id/id/eprint/180403 |
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