Natasha, Doharni Olivia (2019) Pengaruh Derajat Fibrosis Hati terhadap Jumlah Trombosit Pada Tikus Model Fibrosis Hati yang Diberi Ekstrak Daun Moringa oleifera. Sarjana thesis, Universitas Brawijaya.
Abstract
Fibrosis hati merupakan salah satu kondisi dari kerusakan hati yang dimulai dari adanya inflamasi salah satunya karena paparan bahan kimia seperti CCl4. CCl4 dimetabolisme di hati lalu menghasilkan radikal bebas yang menginduksi kerusakan sel hepatosit yang luas. Kerusakan ini dapat dicegah melalui proses regenerasi hati dengan bantuan trombosit. Selain fibrosis hati dapat dicegah dengan pemberian agen hepatoprotektif seperti Moringa oleifera (MO). Penelitian eksperimental laboratorik ini bertujuan mengetahui hubungan jumlah trombosit dengan derajat fibrosis hati pada tikus model fibrosis hati yang diberi ekstrak daun MO. Penelitian ini menggunakan tikus Rattus novergicus strain Wistar sebagai tikus model fibrosis hati yang diinduksi CCl4 dan ada kelompok yang diberikan ekstrak daun MO selama 14 minggu. Hati tikus penelitian diambil untuk memeriksa derajat fibrosis hati darahnya diambil untuk pengitungan jumlah trombosit dan limpanya diambil untuk ditimbang. Pada pengelompokan data berdasarkan kelompok perlakuan, didapatkan hasil uji Kruskal-Wallis mendapatkan nilai p=0,032 (p<0,05) dan hasil uji korelasi Spearman mendapatkan nilai p=0,539 (p>0,05). Pada pengelompokan data berdasarkan derajat fibrosis, didapatkan hasil uji Kruskal-Wallis mendapatkan nilai p=0,081 (p>0,05) dan hasil uji korelasi Spearman mendapatkan nilai p=0,659 (p>0,05) Kesimpulan dari penelitian ini yaitu terdapat kecenderungan bahwa derajat fibrosis hati memiliki pengaruh linier berbanding terbalik terhadap jumlah trombosit pada tikus model fibrosis hati walau secara statistik tidak didapatkan pengaruh signifikan derajat fibrosis hati terhadap jumlah trombosit pada tikus model fibrosis hati yang diberi ekstrak daun MO. Pemberian ekstrak daun MO pun dapat membantu meningkatkan jumlah trombosit pada tikus model fibrosis hati yang diberi ekstrak daun MO.
English Abstract
Liver fibrosis is a condition of liver damage that starts from inflammation, one of which is due to exposure to chemicals such as CCl4. CCl4 is metabolized in the liver and then produces free radicals that induce extensive damage to hepatocyte cells. This damage can be prevented through the process of liver regeneration with the help of platelets. In addition to liver fibrosis can be prevented by giving hepatoprotective agents such as Moringa oleifera (MO). This experimental laboratory study aims to determine the relationship of platelet count with the degree of liver fibrosis in rats with liver fibrosis models who were given MO leaf extract. This study used Rattus novergicus strain Wistar rats as CCl4-induced liver fibrosis mice and there was a group given MO leaf extract for 14 weeks. The rat liver was taken to check the degree of liver fibrosis, its blood was taken for platelet count and the spleen was taken to be weighed. In grouping data based on treatment groups, the Kruskal-Wallis test results obtained p = 0.032 (p <0.05) and the Spearman correlation test results obtained p = 0.539 (p> 0.05). In grouping data based on the degree of fibrosis, Kruskal-Wallis test results obtained p = 0.081 (p> 0.05) and Spearman correlation test results obtained p = 0.659 (p> 0.05) The conclusion of this study is that there is a tendency that the degree of liver fibrosis has a linear effect which is inversely related to the number of platelets in rats with liver fibrosis model although there is no statistically significant effect of the degree of liver fibrosis on platelet count in rats with liver fibrosis model which is given MO leaf extract. The administration of MO leaf extract can also help increase platelet count in rat liver fibrosis models given MO leaf extract.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/410/052001255 |
| Uncontrolled Keywords: | Fibrosis Hati, CCl4, Jumlah Trombosit, Daun Moringa oleifera-Liver fibrosis, CCl4, platelet count, Moringa oleifera leaves |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) > 615.107 24 Experimental research |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 19 Jul 2020 07:22 |
| Last Modified: | 22 Oct 2021 07:26 |
| URI: | http://repository.ub.ac.id/id/eprint/180031 |
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