Purwanto, Firena Kusumaningtyas (2019) Pengaruh Nimodipin Terhadap Reactive Oxygen Species (ROS) pada Kultur Neuron Cell Line SH-SY5Y yang Dipapar Hiperglikemi Kronik. Sarjana thesis, Universitas Brawijaya.
Abstract
Latar belakang : Diabetes melitus merupakan penyakit metabolik dengan kondisi hiperglikemi kronis. Neuropati saraf tepi diabetik ini merupakan kondisi yang timbul dari kerusakan jangka panjang akibat hiperglikemia kronis dan kombinasi berbagai faktor dan jalur. Salah satunya ialah terjadinya stres oksidatif yang diakibatkan peningkatan ROS intrasel. Akumulasi dari ROS dapat mengaktivasi jalur-jalur lain dan mengakibatkan adanya ketidakseimbangan ion Ca2+. Nimodipin merupakan calcium channel blocker tipe L memiliki sifat neuroprotektif. Oleh karenya, nimodipin diharapkan mampu menurunkan ekspresi ROS pada neuron yang terjadi akibat kondisi hiperglikemi kronik. Tujuan : Untuk mengetahui efek nimodipin terhadap ROS pada kultur neuron cell-line SH-SY5Y yang diinduksi hiperglikemi kronik. Metode : Penelitan eksperimentar dengan metode in vitro menggunakan neuronal cell line SH-SY5Y yang dipapar hiperglikemi kronik. Sampel penelitian dibagi menjadi 9 kelompok, kelompok normoglikemi kontrol, hiperglikemi kontrol 25 mM dan 50 mM, normoglikemi dengan nimodipin 10 nM dan 100 nM, hiperglikemi 25 mM dengan nimodipin 10 nM dan 100 nM, dan kelompok hiperglikemi 50 mM dengan nimodipin 10 nM dan 100 nM. Pemberian nimodipin diberikan selama 30 menit pada sel Sh-Sy5Y yang sebelumnya telah dipapar dengan kondisi hiperglikemi kronik selama 6 hari. Intensitas ROS intasel diamati dengan metode imunohistokimia pada hari keenam. Hasil : Pada perbandingan kondisi normoglikemi (5 mM) dan hiperglikemi 25 mM, kondisi normoglikemi 5 mM menghasilkan intensitas ROS yang lebih tinggi secara signifikan (p<0.05). Sedangkan pada perbandingan normoglikemi (5 mM) dan hiperglikemi 50 mM terjadi peningkatan intensitas ROS yang signifikan (p<0.05). Paparan glukosa 5 mM mampu meningkatkan ekspresi ROS akibat terjadinya serangkain proses akibat kondisi hipoglikemi. Paparan glukosa dosis tinggi (50 mM) mampu meningkatkan pembentukan ROS. Pemberian nimodipin pada sel mampu menurunkan intensitas ROS pada paparan glukosa 5 mM, 25 mM dan 50 mM secara signifikan (p<0.05). Kesimpulan : Kondisi hiperglikemi kronik mampu meningkatkan pembentukan ROS pada kultur sel SH-SY5Y dan nimodipin dapat menurunkan kadar ROS secara signifikan.
English Abstract
Background: Diabetes Mellitus is a metabolic disease characterized by hyperglycemia. Diabetic peripheral neuropathy is a condition that arises form long term damage due to chronic hyperglycemia and combination of various factors and pathways. One of them is oxidative stress caused by increased intracellular ROS. The accumulation of ROS can activate other pathways and related to imbalance of Ca2+ ions. Nimodipine is a L-type calcium channel blocker which has neuroprotective properties. Therefore, nimodipin is expected to reduce ROS expression in neurons that occur due to chronic hyperglycemic conditions. Aim: To determine the effect of nimodipine on ROS in SH-SY5Y neuronal cell-line culture induced by chronic hyperglycemia. Methods: Experimental research with in vitro methods was conducted using the SH-SY5Y neuronal cell-line culture exposed to chronic hyperglycemia. The study sample was divided into 9 groups, the normoglycemic control group, hyperglycemia control 25 mM and 50 mM groups, the normoglycemia group with 10 nM and 100 nM nimodipin, 25 mM hyperglycemic goup with 10 nM and 100 nM nimodipine, and the 50 mM hyperglycemic group with 10 nM and 100 nM nimodipine. The administration of nimodipin was given for 30 minutes on SH-SY5Y cells that had previously been exposed to chronic hyperglycemic conditions for 6 days. The intensity of intracellular ROS was observed by immunohistochemical methods on the sixth day. Results: In comparison of normoglycemia (5 mM) and hyperglycemia 25 mM, normoglycemic conditions 5 mM resulted in significantly higher ROS intensity (p <0.05). Whereas in the comparison of normoglycemia (5 mM) and hyperglycemia 50 mM there was also a significant increase in ROS intensity (p <0.05). Exposure to glucose 5 mM can increase ROS expression due to a series of processes in hypoglycemic conditions. Exposure to high doses of glucose (50 mM) can increase ROS formation. The administration of nimodipin to cells was able to significantly reduce the intensity of ROS on glucose exposure of 5 mM, 25 mM and 50 mM (p <0.05) in SH-SY5Y cells. Conclusions: Chronic hyperglycemic conditions can increase the formation of ROS in SH-SY5Y ell culture and nimodipin can significantly reduce ROS levels.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/389/052001234 |
| Uncontrolled Keywords: | Nimodipin, sel SH-SY5Y, ROS, Hiperglikemi, Neuropati-Nimodipine, SH-SY5Y cell line, ROS, Hyperglycemia, Neuropathy |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.4 Diseases of endocrine, hematopoietic, lymphatic, glandular system; diseases of male breast > 616.46 Diseases of islands of Langerhans > 616.462 Diabetes mellitus |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 18 Aug 2020 03:13 |
| Last Modified: | 22 Oct 2021 07:58 |
| URI: | http://repository.ub.ac.id/id/eprint/179880 |
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