Oktaviani, Laksmita Tristy (2019) Potensi Bakteri Penghasil Biosurfaktan sebagai Pendegradasi Solar dari Air Laut di Pelabuhan Tanjung Perak, Surabaya. Sarjana thesis, Universitas Brawijaya.
Abstract
Biosurfaktan merupakan komponen aktif permukaan yang dapat diproduksi oleh beberapa jenis mikroba untuk meningkatkan proses biodegradasi senyawa hidrokarbon seperti solar. Penelitian ini bertujuan untuk mengetahui potensi dan mengidentifikasi bakteri pendegradasi solar yang dapat memproduksi biosurfaktan. Bakteri dalam sampel air laut dikultur dalam media Bushnell Haas Mineral Salts (BHMS) yang telah ditambahkan 1% (v/v) solar kemudian diisolasi dan dimurnikan. Koloni tunggal diamati morfologi koloni dan sel nya. Potensi bakteri menghasilkan biosurfaktan diketahui dengan uji aktivitas pemindahan minyak dan indeks bioemulsi (E24). Isolat potensial diuji kemampuannya dalam mendegradasi solar selama 14 hari kemudian dianalisis Total Petroleum Hydrocarbon (TPH) secara gravimetri dan dilakukan identifikasi berdasarkan sekuen 16S rDNA dan . Hasil isolasi didapatkan 28 isolat bakteri yang memiliki morfologi koloni dan bentuk sel yang beragam. Semua isolat merupakan bakteri Gram negatif. Tiga isolat bakteri (N3, N8 dan K8) menunjukkan zona aktivitas pemindahan minyak antara 2,6-3,5 cm. Hanya lima (K8, K10, N2, N3, N4) dari 28 isolat yang membentuk indeks bioemulsi (E24). Indeks bioemulsi terbesar dimiliki isolat N3 (9,83%). Isolat N3 dan K8 mampu mendegradasi solar lebih dari 40 % berturut-turut sebesar 42,90 % dan 57,43 % dalam waktu 14 hari di media BHMS yang telah ditambahkan solar 1% (v/v). Isolat N3 dan K8 teridentifikasi sebagai Pseudomonas aeruginosa yang berbeda strain dengan nilai similaritas antar isolat pada dendogram sebesar 0,76.
English Abstract
Biosurfactant is an active surface compounds produced by several types of microbes to enhance the biodegradation process of hydrocarbons such as solar. The research aims were to determine the potential and identify diesel degrading bacteria which can produce biosurfactant. Bacteria were enriched in Bushnell Haas Mineral Salts (BHMS) media containing 1% (v/v) of diesel then isolated and purified. Pure colonies were observed for colony and cell morphology. The potential bacteria in producing biosurfactant is known by oil spreading assay and formation of bioemulsion index (E24). Potential isolates were tested for their ability to degrade diesel for 14 days and then analyzed by Total Petroleum Hydrocarbon (TPH) gravimetrically. Identification of potential isolates was based on 16S rDNA sequences. There were 28 isolated bacterial isolates which had diverse colony morphology and cell forms. All isolates were Gram negative bacteria. There were three isolates (N3, N8 and K8) which indicate the zone of oil transfer activity between 2.6-3.5 cm. Only five (K8, K10, N2, N3, N4) of 28 isolates were able to form a bioemulsion index (E24), which the largest index belong to N3 isolate (9.83%). Isolates of N3 and K8 were selected isolates capable to degrade diesel above 40% with a value of 42,90% and 57,43%, respectively after 14 days incubation in BHMS media containing 1% (v/v) of diesel. Isolates of N3 and K8 identified as different strains of Pseudomonas aeruginosa with similarity value 0,76.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2019/353/052001508 |
| Uncontrolled Keywords: | air laut, biodegradasi, biosurfaktan, Pseudomonas aeruginosa, solar, biodegradation, biosurfactant, diesel, Pseudomonas aeruginosa, seawater |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.3 Prokaryotes |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 02 Nov 2020 03:59 |
| Last Modified: | 02 Nov 2020 03:59 |
| URI: | http://repository.ub.ac.id/id/eprint/179834 |
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