Dalilati, Annia Zhafarina (2019) Efek Ekstrak Kopi Robusta ‘Amstirdam’ terhadap Jumlah Relatif Sel CD4+IL-10+ dan CD4+TGF-β+ pada Mencit (Mus musculus) Perlakuan High Fat-Fructose Diet (HFFD). Sarjana thesis, Universitas Brawijaya.
Abstract
HFFD (High Fat-fructose diet) merupakan pemicu penyakit hiperkolesterol dan atherosclerosis yang merupakan awal dari penyakit kardiovaskular. Penanganan penyakit ini umumnya menggunakan statin, namun senyawa ini dapat menimbulkan efek samping berupa kerusakan organ. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak kopi amstirdam terhadap histopatologi jaringan aorta, profil kadar kolesterol darah, dan ekspresi sitokin IL-10 dan TGF-β dari sel CD4+. Mencit dikelompokkan menjadi 5 kelompok (n=5): kontrol normal (KN; non-HFFD), HFFD (pakan high fat-fructose diet), D1(HFFD+ekstrak kopi 0,104 mg/gram BB), D2 (HFFD+ekstrak kopi 0,52 mg/gram BB) dan D3 (HFFD+ekstrak kopi 5,2 mg/gram BB). Pemberian ekstrak kopi dilakukan selama 2 minggu. Setelah 5 bulan, mencit dibedah, sel CD4+IL-10+ dan CD4+TGF-β+ dari limpa dianalisis menggunakan flow cytometri serta aorta untuk dianalisis histopatologinya. Hasil flow cytometri dilanjutkan dengan analisis statistik menggunakan One Way ANOVA dengan nilai α= 5% dan uji Tukey. Berdasarkan hasil penelitian, diperoleh level ekspresi IL-10 terendah terdapat pada kelompok mencit D1 (6,55%) yang tidak berbeda nyata dengan kelompok HFFD (6,64%) dan tertinggi terdapat pada kelompok D2 (15,47%) dan kelompok D3 (9,17%). Level ekspresi TGF-β terendah terdapat pada kelompok D2 (4,15%) yang tidak berbeda nyata dengan HFFD (4,73%) sedangkan ekspresi tertinggi terdapat pada D3 (8,72%) dan D1 (6,17%). Ekstrak kopi juga dapat mencegah atherogenesis ditandai dengan berkurangnya jumlah relatif foam cell pada dinding aorta.
English Abstract
HFFD (High fat-fructose diet) is the trigger of hypercholesterolemia or atherosclerosis and the early stage of cardiovascular disease. Generally, statin are used as common therapy for this disease, but this compound also cause several side effects leading to organ damages. The aims of this research are to know the potential effect of coffee extract on aorta histopathology, lipid profile, and IL-10 and TGF-β level expressed by CD4+ T cell of spleen. Mice were divided into 5 groups (n=5): Normal control (KN; fed with non-high fat fructose diet), HFFD (fed with high fat fructose diet), D1 (HFFD+coffee extract 0,104 mg/gram BW), D2 (HFFD+coffee extract 0,52 mg/gram BW), and D3 (HFFD+coffee extract 5,2 mg/gram BW). Coffee extract were given for 2 weeks. After 5 months, mice were dissected and spleen were isolated to analyze the expression of IL-10 and TGF-β level of CD4+ T cell using flow cytometry. The relative number of expression of IL-10 and TGF-β were then statistically analyzed by One Way ANOVA with significance level of 5%, then continued by Tukey HSD test. The result showed that the lowest level of expression of IL-10 is D1 group (6,55%) that was not significantly different with HFFD group (6,64%) and the highest level of expression was D2 group (15,47%) followed by D3 group (9,17%). The lowest expression level of TGF-β showed by D2 group (4,15%) that was not significantly different with HFFD group (4,73%). The highest level of expression of TGF-β showed by D3 group (8,72%) followed by D1 group (6,17%). Coffee extract also reduced the relative amount of foam cells accumulating in aortic wall.
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2019/257/051911037 |
| Uncontrolled Keywords: | Atherosclerosis, ekstrak kopi, HFFD, sitokin, Atherosclerosis, coffee extract, cytokine, HFFD |
| Subjects: | 500 Natural sciences and mathematics > 543 Analytical chemistry > 543.599 3 Analytical chemistry (Mammals-Mouse) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 02 Oct 2020 02:49 |
| Last Modified: | 02 Oct 2020 02:49 |
| URI: | http://repository.ub.ac.id/id/eprint/179630 |
Actions (login required)
 |
View Item |