Wibisono, Chasan Mirza (2019) Uji Toleransi dan Penurunan Konsentrasi Tembaga (Cu) oleh Bakteri Indigenous Air Lindi dari Tempat Pembuangan Akhir (TPA) Supit Urang, Kota Malang. Sarjana thesis, Universitas Brawijaya.
Abstract
Air lindi di TPA Supit Urang hanya dialirkan ke kolam penampungan akhir terbuka tanpa ada pengolahan lebih lanjut sehingga kontaminan yang terkandung cukup besar, khususnya logam berat. Tujuan penelitian ini adalah mendapatkan bakteri indigenous air lindi TPA Supit Urang yang toleran terhadap logam Cu dan menguji potensinya dalam menurunkan konsentrasi Cu. Bakteri diisolasi dari sampel air lindi TPA Supit Urang Kota Malang menggunakan medium NA yang mengandung 100 mg/L Cu. Isolat toleran Cu diseleksi dengan metode streak kuadran pada medium NA dengan konsentrasi Cu bertingkat. Kurva pertumbuhan masing-masing isolat dibuat untuk menentukan umur inokulum dan waktu panen pada uji penurunan konsentrasi Cu. Uji penurunan tersebut menggunakan medium NB yang mengandung 50 mg/L Cu dan diukur menggunakan AAS. Berdasarkan hasil analisis, kandungan Cu di air lindi TPA Supit Urang mencapai 13,2 mg/L yang berarti melewati standar baku mutu air lindi dari Kementerian Lingkungan dan Kehutanan. Sebanyak 40 isolat toleran logam Cu telah diisolasi dan terdapat 4 isolat (A16.b, B2, B9 dan B11) yang memiliki kemampuan toleransi maksimal Cu hingga 400 mg/L. Isolat B2 memiliki kemampuan tertinggi dalam menurunkan konsentrasi Cu hingga 33,4 %. Isolat B2 teridentifikasi sebagai Spesies Enterobacter cloacae subsp cloacae dengan nilai similaritas 99,3 %.
English Abstract
Leachates in Supit Urang landfill area is only flowed to settling pond and there is no futher processing so that it can be contaminated by pollutant quite large, especially heavy metals. The objectives of this study was to obtain Cu tolerance indigenous bacteria from leachate in TPA Supit Urang and tested its potential in reducing Cu concentration. Bacteria was isolated from leachate sample in TPA Supit Urang Malang with NA medium containing 100 mg/L Cu. Screening of tolerance Cu isolates was carried out by quadrant streak on NA medium with multilevel concentration of Cu. The growth curve of each isolates from screening step before were constructed to determenine the age of inoculum and harvesting time on removal eficiency test. AAS method use in removal test of Cu to measure the concentration of Cu itself. Based on results, leachate from Supit Urang landfill contain 13.2 mg/L Cu which meant that break the quality standard of leachate from The Ministry of Environment and Forestry. A total of 40 isolates were isolated and there are four isolates (A15, A16.b, B2, B9 and B11) that had high maximum tolerance concentration Cu up to 400 mg/L. Removal test showed that B2 isolates is the highest in remove Cu concentration to 33.4%. B2 isolates was identified as Enterobacter cloacae subsp. cloacae with a similiarity value 99.3 %.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2019/443/052001598 |
| Uncontrolled Keywords: | air lindi, bakteri toleran logam Cu, TPA Supit Urang, Uji penurunan logam Cu, copper tolerance bacteria, leachate, removal eficiency of Cu, Supit Urang landfill, |
| Subjects: | 500 Natural sciences and mathematics > 546 Inorganic chemistry > 546.3 Metals, metallic compounds, alloys |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 10 Aug 2020 08:07 |
| Last Modified: | 10 Aug 2020 08:07 |
| URI: | http://repository.ub.ac.id/id/eprint/179313 |
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