Febriansyah, Muhammad (2019) Daya Antagonisme Rizobakteri Terhadap Bakteri Patogen Ralstonia Solanacearum Dan Potensi Listrik Yang Dihasilkan. Sarjana thesis, Universitas Brawijaya.
Abstract
Rizobakteri merupakan kelompok bakteri yang hidup pada daerah perakaran tumbuhan (rizosfer). Bakteri rizosfer yang berhasil dieksplorasi Sari (2018) diantaranya bakteri dengan genus Bacillus, Clostridium, Xanthomonas, Pseudomonas, Pantoea dan Erwinia namun belum diketahui kemampuannya sebagai agens antagonis. Ralstonia solanacearum merupakan bakteri patogen tular tanah nonflourescen dari famili Pseudomonas. Disi lain beberapa genus bakteri yang tergolong kedalam rizobakteri berpotensi sebagai biokatalis dalam menghasilkan listrik melalui sebuah sistem yang disebut microbial fuel cell (MFC). MFC merupakan perangkat elektrokimia yang mengonversi energy kimia menjadi listrik. Oleh karena itu peneliti akan mengkaji potensi bakteri rizosfer sebagai agens antagonis dan potensinya dalam menghasilkan energi listrik melalui sistem MFC, serta perbedaan listrik yang dihasilkan antara bakteri antagonis dengan bakteri patogen R. solanacearum. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya. Pelaksanaan penelitian memiliki 4 tahap yaitu 1). Persiapan meliputi perakitan perangkat MFC satu bejana, persiapan isolate dan sterilisasi alat dan bahan. 2). Pengujian hipersensitif dan virulensi, pengujian antagonis dan pengujian listrik. 3). Pengamatan arus listrik, waktu, pH, nilai OD, diameter zona bening dan gejala nekrosis pada daun. 4). Analisis data. Hasil penelitian menunjukkan bakteri R. solanacearum bereaksi positif terhadap uji hipersensitif pada daun tembakau. Hasil pengujian antagonis menunjukanbahwa semua isolat rizobakteri mampu menghasilkan zona hambat. Pada pengamatan 24 jam indeks penghambatan Bacillus (N3) berbeda nyata dengan semua perlakuan. Sedangkan indeks penghambatan Clostridium (N10), Xanthomonas (N23), Pseudomonas (N24), dan Pantoea (N26) tidak berbeda nyata. Pada pengamatan 48 jam indeks penghambatan Bacillus (N3), Clostridium (N10), Pseudomonas (N24), dan Erwinia (N27) tidak berbeda nyata, namun Bacillus N3 berbeda nyata dengan Xanthomonas (N23), dan Pantoea (N26). Sehingga, isolat Bacillus (N3) dipilih dalam pengujian potensi listrik dan dibandingkan dengan patogen R. solanacearum. Hasil penelitian bahwa kedua bakteri memiliki potensi dalam menghasilkan listrik. Kuat listrik yang dihasilkan oleh kedua bakteri berbeda. Voltase tertinggi yang dihasilkan oleh Bacillus sebesar 170 mV. Sedangkan, voltase tertinggi yang dihasilkan oleh R.solanacearum sebesar 60 mV. Berdasarkan uji T listrik yang dihasilkan antara bakteri patogen dan bakteri antagonis berbeda nyata. Nilai kerapatan bakteri ditinjau dari nilai OD mengalami peningkatakan di masing-masing perlakuan. Sedangkan nilai pH pada subtrat kedua perlakuan juga mengalami perubahan. Pada perlakuan R.solanacearum pH mengalami penurunan sedangkan pada perlakuan Bacillus N3 mengalami peningkatan. Peningkatan kerapatan bakteri dan perubahan nilai pH berpengaruh terhadap listrik yang dihasilkan.
English Abstract
Rhizobacteria is a group of bacteria that live in rooting regions (rhizosphere). Rhizosphere bacteria which successfully explored Sari (2018) among bacteria genus Bacillus, Clostridium, Xanthomonas, Pseudomonas, Pantoea, and Erwinia yet unknown ability as antagonist agents. Ralstonia solanacearum is a soil-borne pathogenic bacteria nonflourescen of the Pseudomonas family. Several genera of bacteria classified as rhizobacteria have potential as biocatalysts to produce electricity with a microbial fuel cell (MFC) system. The MFC is an electrochemical device that converts chemical energy into electricity. Therefore, researchers will examine the potential of rhizosphere bacteria as antagonistic agents and their potential in producing electrical energy with MFC system, as well as the difference in electricity produced between antagonistic bacteria and pathogenic bacteria R. solanacearum. The research take place in the Laboratory of Plant Diseases, Agriculture faculty, Brawijaya University. The research has 4 steps, there are 1). Preparations include assembly of a single-vessel MFC device, preparation of isolates and sterilization of tools and materials. 2). Hypersensitivity and virulence testing, antagonistic testing and electrical testing. 3). Observation of electric current, time, pH, OD value, the diameter of clear zone and symptoms of necrosis in leaves. 4). Data analyze. The results showed that R. solanacearum bacteria reacted positively to the hypersensitivity test on tobacco leaves. The results of antagonistic tests on some rhizosphere bacteria including Bacillus (N3), Clostridium (N10), Xanthomonas (N23), Pseudomonas (N24), Pantoea (N26), and Erwinia (N27) that all bacterial isolates were able to produce inhibitory zones. A 24-hour observation, the Bacillus inhibition index (N3) was significantly different from all treatments. Whereas Clostridium (N10), Xanthomonas (N23), Pseudomonas (N24), and Pantoea (N26) indices are not significantly different. At 48 hours of observation, Bacillus (N3), Clostridium (N10), and Erwinia (N27) inhibition indices were not significantly different, but Bacillus (N3) was significantly different from Xanthomonas (N23), Pseudomonas (N24), and Pantoea (N26). Thus, the isolate Bacillus (N3) was selected in testing the electrical potential and compared with the R. solanacearum pathogen. The result of this research shows that both bacteria have the potential to produce electricity. The electric strength produced by the two different bacteria. The highest voltage produced by Bacillus was 170 mV at 52, 56, and 64 hours of observation. Meanwhile, the highest voltage produced by R. solanacearum was 60 mV at 72 hours of observation. Based on the T-test the electricity generated between pathogenic bacteria and antagonistic bacteria is significantly different. Bacterial density values in terms of OD values experienced an increase in each treatment. While the pH value in the two substrate treatments also changed. In the R. solanacearum treatment the pH decreased while in the Bacillus (N3) treatment is increased. Increased bacterial density and changes in pH value affect the electricity produced.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2019/865/052000959 |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 02 Oct 2020 02:28 |
| Last Modified: | 28 Oct 2021 01:36 |
| URI: | http://repository.ub.ac.id/id/eprint/179152 |
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