Aritonang, Chintya Dewi Novyanti (2019) Efektivitas Ekstrak Etanol Daun Alpukat (Persea Americana Mill) dalam Menghambat Pertumbuhan Aggregatibacter Actinomycetemcomitans secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Periodontitis agresif adalah penyakit rongga mulut yang disebabkan oleh bakteri patogen periodontal, terutama Aggregatibacter actinomycetem-comitans. Salah satu bahan yang memiliki sifat sebagai antibakteri adalah daun alpukat karena mengandung senyawa flavonoid, fenol, saponin, alkaloid, dan tannin. Tujuan penelitian ini yaitu untuk mempelajari efektivitas ekstrak etanol daun alpukat (Persea americana mill) dalam menghambat pertumbuhan bakteri Aggregatibacter actinomycetemcomitans secara in vitro. Penelitian ini menggunakan true experiment dengan pendekatan post test only control group design, menggunakan metode difusi sumuran untuk membuktikan kemampuan antibakteri ekstrak etanol daun alpukat. Konsentrasi daun alpukat yang digunakan adalah 50%, 60%, 70%, 80%, 90%, 100%, aquades sebagai kontrol negatif, dan chlorhexidine gluconate 0,2% sebagai kontrol positif. Hasil analisis data menggunakan One-way ANOVA menunjukkan hasil signifikansi 0,000 (p<0,05) yang berarti adanya perbedaan yang signifikan antara pemberian ekstrak daun alpukat dalam berbagai konsentrasi terhadap zona hambat bakteri Aggregatibacter actinomycetemcomitans. Hasil uji Korelasi Pearson didapatkan nilai signifikansi 0,000 (p<0,05) yang berarti meningkatnya konsentrasi ekstrak daun alpukat dapat meningkatkan diameter zona hambat bakteri Aggregatibacter actinomycetemcomitans. Hal ini terjadi karena adanya peningkatan konsentrasi ekstrak daun alpukat, maka kandungan kimia aktif di dalam ekstrak daun alpukat juga meningkat. Kesimpulan penelitian ini adalah ekstrak daun alpukat tidak efektif dalam menghambat pertumbuhan bakteri Aggregatibacter actinomycetem-comitans secara in vitro.
English Abstract
Aggressive periodontitis is periodontal disease caused by periodontal pathogenic bacteria, especially Aggregatibacter actinomycetemcomitans. One of ingredient that has antibacterial properties is avocado leaves extract because it contains compounds of flavonoids, phenols, saponins, alkaloids, and tannins. The purpose of this research was to studying the effectiveness of avocado leaves (Persea americana mill) ethanol extract in inhibiting the growth of Aggregatibacter actinomycetemcomitans bacteria by in vitro. This research used true experiment with the post test only control group design approach, with a well diffusion method to determine inhibition zones of bacterial. The concentrations of avocado leaves used were 50%, 60%, 70%, 80%, 90%, 100%, aquades as negative control, and chlorhexidine gluconate 0.2% as positive control. The result of data analysis used One-way ANOVA with a significance value of 0,000 (p <0.05) which showed a significant difference between the avocado leaf extract in various concentrations against the inhibition zone of Aggregatibacter actinomycetemcomitans bacteria. The Pearson Correlation test results obtained a significance value of 0,000 (p <0.05) which means that increasing the concentration of avocado leaf extract can increase the inhibition zone diameter of Aggregatibacter actinomycetemcomitans bacteria. This occurs because of an increase in the concentration of avocado leaf extract, so the active chemical content in avocado leaves extract also increases. The conclution of this study is avocado leaf extract is not effective in inhibiting the growth of Aggregatibacter actinomycetemcomitans bacteria in vitro.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKG/2019/125/052001887 |
| Uncontrolled Keywords: | Aggregatibacter actinomycetemcomitans, ekstrak daun alpukat, zona hambat, difusi sumuran Aggregatibacter actinomycetemcomitans, avocado leaf extract, inhibition zone, well diffusion |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry > 617.66 Extraction / Teeth--Extraction |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 10 Aug 2020 07:41 |
| Last Modified: | 23 Oct 2021 02:31 |
| URI: | http://repository.ub.ac.id/id/eprint/178899 |
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