Rahmawan, Eki Reza (2019) Efek Preventif Virgin Coconut Oil (Vco) Hasil Fermentasi Lactobacillus Casei Terhadap Kadar Superoxide Dismutase Dan Histopatologi Hepar Tikus (Rattus Novergicus) Yang Diinfeksi Salmonella Enteritidis. Sarjana thesis, Universitas Brawijaya.
Abstract
Salmonellosis merupakan penyakit yang dapat menyebabkan gangguan saluran pencernaan atau gastroenteritis pada manusia dan hewan. Sampai saat ini pencegahan yang diberikan dengan menjaga higienitas dan biosecurity, sehingga diperlukan adanya alternatif pencegahan yang berasal dari bahan alami. Virgin Coconut Oil (VCO) fermentasi Lactobacilus casei adalah food supplement mengandung asam laurat (43-53%). Bakteri asam laktat sebagai probiotik dan antioksidan mampu menghambat pertumbuhan bakteri patogen. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian VCO (Virgin Coconut Oil) fermentasi Lactobacillus casei sebagai pencegahan terhadap penyakit saluran pencernaan yang disebabkan oleh Salmonella sp. Penelitian ini bersifat true experimental menggunakan Rancangan Acak Lengkap (RAL). Hewan model menggunakan tikus putih (Rattus norvegicus) strain Wistar jantan berumur 8 - 12 minggu dengan berat badan sekitar 150 g diadaptasikan selama 7 hari dan dibagi dalam lima kelompok perlakuan. Kelompok negatif, positif, dan kelompok perlakuan 1, 2, dan 3 yang disuplementasi VCO fermentasi Lactobacilus casei dengan dosis masing-masing 3.4, 6.7, dan 10 mL/kgBB secara per oral selama 7 hari. Pada hari ke 15, hewan coba ditantang dengan bakteri S. enteritidis 1 ml konsentrasi 108cfu/ml. Kadar SOD diukur menggunakan spektofotometri, analisa data dilakukan secara kuantitatif menggunakan uji One Way ANOVA, α = 5%. Histopatologi hepar diwarnai dengan Hematoxyline Eosin (HE) dan dianalisis secara deskriptif untuk melihat hambatan kerusakan hepar. Kesimpulan penelitian bahwa pemberian Virgin Coconut Oil (VCO) hasil fermentasi Lactobacillus casei pada tikus (Rattus novergicus) dengan dosis 6,7 mL/kgBB dapat mencegah terjadinya salmonellosis pada hewan coba tikus (Ratus norvegicus) yang diinduksi Salmonella enteritidis, berdasarkan peningkatan kadar enzim Superoxide Dismutase (SOD) dan mencegah rusaknya gambaran rusaknya sel hepatosit, sinusoid dan infiltrasi sel inflamasi pada histopatologi hepar tikus.
English Abstract
Salmonellosis is a disease that can caused digestive disorder or gastroenteritis on both animal and human. To this date, the preventive measurements against salmonellosis is to pay attention on hygiene and biosecurity, so there’s a need to find an alternative for preventive measure from a natural resource. Virgin Coconut Oil (VCO) fermented by Lactobacillus casei categorized as food supplement containing lauric acid (45-53%). Lactic acid bacteria used as probiotic and antioxidant can stall the growth of pathogenic bacteria. This research aim is to find the effect of VCO fermented by Lactobacillus casei for preventing digestive disease that were caused by Salmonella sp. This research is a true experimental and use a Complete Randomized Design (CRD). The animal model that were used in this study were 150 g 8-12 weeks wistar strain albino rats (Rattus norvegicus) that were acclimatized for 7 days and divided into five group. Negative, positive, treatment group 1, 2 and 3 that were supplemented with fermented VCO with Lactobacillus casei with 3.4, 6.7, dan 10 mL/kg weight, respectively, orally administered for 7 days. The 15th day, the animal model were challenged with a 1ml Salmonella enteritidis with a 108 cfu/ml concentration. SOD level were measured with spectrophotometer, then analyzed quantitatively using One Way ANOVA with an α = 5%. The liver histopathology were stained using Hamatoxyline Eosin (HE) and analyzed descriptively to see the delayed liver damage. The conclusion of the effects of Virgin Coconut Oil (VCO) fermented by Lactobacillus casei in rats (Rattus novergicus) at a dose of 6,7 mL/kg weight can prevent salmonellosis against Salmonella enteritidis induced rat (Ratus norvegicus), based on increased levels of Superoxide Dismutase (SOD) and inhibited the damage to hepatocyte cells, sinusoids and infiltration of inflammatory cells in rat liver histopathology.
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/158/051909096 |
| Uncontrolled Keywords: | Tikus, VCO Fermentasi, Salmonellosis, Radikal bebas, Histopatologi Hepar, Rats, Fermented VCO, Salmonellosis, Free radical, Histopathology, Liver. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) > 615.107 24 Experimental research |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:46 |
| Last Modified: | 21 Oct 2021 06:21 |
| URI: | http://repository.ub.ac.id/id/eprint/177018 |
Preview |
Text
EKI REZA RAHMAWAN (2).pdf Download (2MB) | Preview |
Actions (login required)
 |
View Item |