Ora, Krismal Marshel (2019) Potensi Daun Gamal (Giliciridia Sepium) Dalam Sedian Topikal Poultice Pada Luka Insisi Tikus (Rattus Norvegicus) Terhadap Ekspresi Il-6 Dan Ketebalan Epidermis. Sarjana thesis, Universitas Brawijaya.
Abstract
Dokter hewan sering melakukan Luka bedah incisi pada operasi laparatomi. Luka bedah yang lama sembuh akan menyebabkan stress pada hewan. Daun Gamal (Giliciridia sepium) memiliki kandungan zat aktif yaitu flavonoid, saponinn, tanin sebagai anti Inflamasi dalam membantu proses regenerasi jaringan, sehingga mempercepat waktu penyembuhan dengan pembentukan Ketebalan Epidermis. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Sediaan Topikal Poultice Daun Gamal (Giliciridia sepium) terhadap ekspresi Interleukin-6 (IL-6) dan Ketebalan Epidermis Luka Insisi selama proses penyembuhan Luka. Hewan coba yang digunakan adalah 20 ekor Tikus Strain Wistar umur 8-12 minggu dibagi 4 kelompok yaitu kelompok kontrol negatif dengan Tikus sehat, kelompok kontrol positif dengan model Luka Insisi dengan Luka dijahit tanpa diberikan terapi Sediaan Topikal Poultice Daun Gamal, kelompok perlakuan dengan terapi Sediaan Topikal Poultice Daun Gamal Indonesia dengan Luka dijahit, kelompok perlakuan dengan terapi Sediaan Topikal Poultice Daun Gamal Filipina dengan Luka dijahit. Variabel yang diamati dalam penelitian ini adalah ekspresi IL-6 dengan menggunakan metode Immunohistokimia dan diukur dengan ImmunoRatio dan Ketebalan Epidermis yang diukur secara mikroskopik melalui preparat HE. Data kemudian disajikan dalam bentuk tabel distribusi frekuensi. Data kuantitatif dianalisa statistik ANOVA dilanjutkan uji Tukey α = 0,05. Hasil penelitian menunjukkan bahwa Sediaan Topikal Poultice Daun Gamal secara signifikan (p<0,05) menurunkan ekspresi IL-6 dan meningkatkan Ketebalan Epidermis pasca Luka Insisi. Kesimpulan dari penelitian ini yaitu Sediaan Topikal Poultice Daun Gamal baik Indonesia dan Filipina mampu dalam mendukung proses penyembuhan Luka Insisi karena dapat menurunkan ekspresi IL-6 dan meningkatkan Ketebalan Epidermis
English Abstract
Veterinarians often perform incision procedure in laparotomy surgery. Longcured surgical wounds will cause stress to animals. Leaves Gamal (Giliciridia sepium) has an active ingredient, namely flavonoids, saponinn, tannins as antiinflammatory in helping the tissue regeneration process, thus accelerating healing time by forming Epidermal Thickness. This study aimed to determine the effect of Topical Poultice Supply administration on Gamal Leaves (Giliciridia sepium) on the expression of Interleukin-6 (IL-6) and Epidermal Thickness of incision wounds during the wound healing process. The experimental animals used 20 Wistar Strains rats (Rattus norvegicus) aged 8-12 weeks and divided into 4 groups, namely the negative control group with normal rats, the positive control group with incision wound models with sutures sutured without Topical Poultice treament Gamal Leaves, the treatment group with Supply therapy Topical Poultice Indonesia Gamal Leaves with sutured wounds, treatment group with Topical Poultice Supply therapy Philippine Gamal Leaves with sutured wounds. The variable observed in this study was the expression of IL-6 using the Immunohistochemistry method and measured by ImmunoRatio and Epidermal Thickness measured microscopically through the preparations HE. Data was presented in the form of a frequency distribution table Quantitative data analyzed statistically ANOVA followed by Tukey test α = 0.05. The results showed that the Topical Poultice of Gamal Leaves significantly (p <0.05) reduced the expression of IL-6 and increased Epidermal Thickness in incision wounds. The conclusion of this study was the Topical Poultice of Gamal Leaves both from Indonesia and the Philippines are able to support the incision wound healing process because it reduced the expression of IL-6 and increased Epidermal Thickness.
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/86/051909024 |
| Uncontrolled Keywords: | Luka Insisi, Daun Gamal, Flavonoid, IL-6, Ketebalan Epidermis, Wound incision, Gamal Leaves, Flavonoids, IL-6, Epidermis Thickness |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) > 615.107 24 Experimental research |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:43 |
| Last Modified: | 21 Oct 2021 03:54 |
| URI: | http://repository.ub.ac.id/id/eprint/177007 |
Preview |
Text
KRISMAL MARSHEL ORA (2).pdf Download (2MB) | Preview |
Actions (login required)
 |
View Item |