Soearto, Talita Sari (2019) Pengaruh Pemberian Pakan Diet Tinggi Lemak Terhadap Kadar Hdl (High Density Lipoprotein) Dan Ldl (Low Densitylipoprotein) Tikus (Rattus Norvegicus) Yang Diberipaparan Immobilization Stress. Sarjana thesis, Universitas Brawijaya.
Abstract
Kondisi stress yang ditunjang dengan asupan makanan tinggi akan lemak menyebabkan perubahan kadar HDL (High Density Lipoprotein) dan LDL (Low Density Lipoprotein) mengalami perubahan. Penelitian ini bertujuan mengetahui pengaruh pemberian pakan diet tinggi lemak terhadap kadar HDL (High Density Lipoprotein) dan LDL (Low Density Lipoprotein) yang diberi paparan immobilization stress ini menggunakan hewan coba tikus (Rattus norvegicus). Penelitian ini menggunakan tikus yang dibagi menjadi 4 kelompok perlakuan: Kelompok kontrol negatif, tikus kelompok 1 dengan pemberian perlakuan diet tinggi lemak sonde lambung sebanyak 5 mL/ekor/hari dengan pemberian 3 kali sehari, yaitu pagi hari sebanyak 2 mLpukul 07.30, siang hari 1 mLpukul 12.30, dan sore hari 2 mLpukul 16.30, tikus kelompok 2 dengan pemberian perlakuan immobilization stress yaitu tikus dimasukkan kedalam tabung 30 cm x 10 cm diberikan waktu pagi hari yaitu pukul 08.00-11.00 dan siang hari saat pukul 13.00-16.00, tikus kelompok 3 dengan pemberian perlakuan kombinasi pakan diet tinggi lemak dan immobilization stress. Kadar HDL (High Density Lipoprotein) dan LDL (Low Density Lipoprotein) diukur dengan metode spektrofotometri, data dianalisa dengan menggunakan uji ANOVA dilanjutkan uji Tukey (p<0,05).Hasil dari penelitian ini yaitu kadar LDL yang diberi induksi diet tinggi lemak naik secara signifikan dibandingkan dengan kelompok kombinasi. Kadar HDLyang diberi perlakuan immobilization stress menurun signifikan dibandingkan dengan kelompok kombinasi dikarenakan pengaruh dari kortisol. Kesimpulannya yaitu perlakuan kombinasi dapat menyebabkan kenaikan kadar LDL dan menurunkan kadar HDL dalam darah.
English Abstract
The stress conditions supported by high dietary intake of fat will cause changes in the levels of HDL (High Density Lipoprotein) and LDL (Low Density Lipoprotein). This research aims to know the influence of feeding a diet high in fat against the levels of HDL (High Density Lipoprotein) and LDL (Low Density Lipoprotein) were given exposure to stress this immobilization using animals try to rat (Rattus norvegicus). This research uses mice divided into 4 treatment groups: the negative control group, group 1 rats by administering high-fat diet treatment of gastric sonde as much as 5 mL/tail/day with the giving of the 3 times a day, IE2 mL at 07.30, 1 mL at 12.30, and 2 mL at 16.30, Group 2 rats by administering treatment immobilization stress that is inserted into the rats tube 20 cm x 5 cm given time i.e. morning at 08.00-11.00 and daylight time 13.00-16.00, 3 groups of rats by administering treatment the combination of a high-fat diet and feed of immobilization stress. The levels of HDL (High Density Lipoprotein) and LDL (Low Density Lipoprotein) measured with spectrophotometry method, data were analyzed by using ANOVA test followed a test of Tukey (p < 0.05).The result of stress immobilization studies has the influence that HDL levels decreased after the treatment of the immobilization of stress. High-fat diets are able to significantly increase LDL levels in the blood. Based on this study it can be concluded that the immobilization of stress and high-fat diets can affect HDL and LDL levels in blood.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/60/051908998 |
| Uncontrolled Keywords: | Tinggi lemak, Rattus norvegicus, HDL, LDL, Rattus norvegicus, high-fat, HDL, LDL. |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:05 |
| Last Modified: | 21 Oct 2021 03:19 |
| URI: | http://repository.ub.ac.id/id/eprint/176793 |
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