Utami, Nyoman Putri (2019) Efek Preventif Madu Hutan Riau Terhadap Kadar Alkaline Phosphatase (Alp) Dan Histopatologi Hepar Pada Tikus Putih(Rattus Norvegicus) Yang Dipapar Asap Plastik. Sarjana thesis, Universitas Brawijaya.
Abstract
Hepar merupakan organ yang mempunyai fungsi detoksifikasi, termasuk mendetoksifikasi senyawa toksik endogen dan eksogen. Radikal bebas diketahui memiliki peran dalamterjadinya berbagai penyakit termasuk kerusakan hepar pada hewan. Tubuh membutuhkan antioksidan untuk mengurangi kemampuan merusak sel dari radikal bebas. Salah satu sumber antioksidan yaitu madu hutan Riau yang memiliki kandungan flavonoid.Penelitian ini bertujuan untuk mengetahui pengaruh preventif madu hutan Riau terhadap histopatologi hepar tikus yang diinduksi asap plastikuntuk mencegah peningkatan kadar ALP serum darah tikus. Hewan coba yang digunakan dalam penelitian ini adalah tikus putih (Rattus norvegicus) jantan berumur 2-3 bulan dengan berat 150-200 gram. Tikus dibagi menjadi lima kelompok perlakuan yaitu, kelompok P1 (kontrol negatif), kelompok P2 (kontrol positif), kelompok perlakuan yaitu tikus yang diberi tindakan preventif madu hutan Riau pada kelompok P3 dengan konsentrasi 0,25% (v/v) sebanyak 1mL,kelompok P4 dengan konsentrasi 0,50% (v/v) sebanyak 1mL, dan kelompok P5 dengan konsentrasi 0,75% (v/v) sebanyak 1mL. Tikus putih (Rattus norvegicus) diberi madu setiap hari selama 21 hari secara peroral dan dipapar asap plastik dari hasil pembakaran plastik jenis PET selama 20 menit pada pukul 14.00 WIB pada hari ke-14 hingga ke-21, lalu di bedah 24 jam kemudian. Analisa kualitatif digunakan untuk menganalisa gambaran histopatologi hepar dan kadar ALPdiukur secara kuantitatif menggunakan spektrofotometer serta dianalisisstatistika dengan uji One-Way ANOVA, α = 5%.Hasil penelitian menunjukan pemberian preventif maduhutan Riau dapat secara signifikan (p<0,05) mencegah peningkatan kadar ALP yang dipapar asap plastik. Konsentrasi0,75% adalah konsentrasipemberian madu yang terbaik untuk mencegah nekrosis di hepatosit pada hepar yang dipapar asap plastik.
English Abstract
Liver is an organ used for detoxification, including the detoxification of endogenous and exogenous toxic compounds. Free radicals have been known to have a role in occurrence of many diseases including hepatic damage in animals. The body needs antioxidant to decline the ability of free radicals to harm the cells. One of the antioxidant resources is forest honey of Riau that contains flavonoid compound. The purpose of this study was to find out the preventive effect of Riau forest honey towards the liver of rats inducted by plastic fumes to prevent the level of rats’ blood serum ALP to increase. The animals used in this study were male rats (Rattus norvegicus) 2-3 months of age and weighed around 150-200 grams. The rats were divided into five groups consisted of P1 group (negative control), P2 group (positive control), treatment group (P3 group) that was given Riau forest honey as a preventive action with the concentration of 0,25% (v/v) in 1 mL, P4 group with the concentration of 0,50% (v/v) in 1 mL, and P5 group with the concentration of 0,75% (v/v) in 1 mL. The rats were given honey daily for 21 days, orally and exposed by plastic fumes from the result of PET plastic burning for 20 minutes at 09.00 AM and 02.00 PM on the 15th day until the 21st day, continued by a dissection 24 hours later. The histopathology of hepar was descriptively analized with microscope. ALP levels were measured quantitatively using spectrophotometer and were analyzed statistically with OneWay ANOVA method, α = 5%. The result of this study showed that the administration of Riau forest honey preventive therapy could significantly (p<0,05) prevent the levels of ALP to increase in rats’ blood serum and prevent liver cells necrosis exposed by plastic fumes. The concentration of 0, 75% was the best honey concentration to prevent ALP levels to increase and prevent liver cells necrosis
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/128/051909058 |
| Uncontrolled Keywords: | antioksidan, madu hutan Riau, radikal bebas, ALP, histopatologi hepar, antioxidant, Riau forest honey, free radicals, ALP, histophatology liver |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 05:54 |
| Last Modified: | 21 Oct 2021 04:27 |
| URI: | http://repository.ub.ac.id/id/eprint/176766 |
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