Qoys, Fuad Nabil (2019) Pengaruh Pemberian Ekstrak Kulit Jeruk Manis (Citrus Sinensis) Secara Oral Dan Topikal Terhadap Ekspresi Igf-1 Dan Jumlah Sel Mononuklear Pada Penyembuhan Luka Tikus (Rattus Norvegicus) Model Diabetes Mellitus Hasil Induksi Streptozotosin. Sarjana thesis, Universitas Brawijaya.
Abstract
Luka pada penderita DM mengakibatkan fase inflamasi berkepanjangan karena tingginya stres oksidatif yang mengakibatkan terjadi gangguan aktivitas IGF-1 dan meningkatnya adhesi sel MN pada area luka. Kulit jeruk manis diketahui mengandung flavonoid yang memiliki sifat antidiabetik dan antiinflamasi. Penelitian ini bertujuan untuk mengetahui ekspresi IGF-1 dan jumlah sel MN pada luka insisi yang telah di terapi menggunakan salep dan effervescent ekstrak kulit jeruk manis. Hewan model yang digunakan adalah tikus (Rattus norvegicus) jantan strain Wistar berat 150-200 gram yang dibagi dalam 5 kelompok masing-masing 4 ekor diantaranya kontrol negatif insisi tanpa DM, kontrol positif insisi kondisi DM, terapi 1 insisi kondisi DM diterapi effervescent 500 mg/kgBB, terapi 2 diterapi salep 5%, terapi 3 kombinasi. Terapi dilakukan pada hewan tikus model DM hasil induksi streptozotocin melalui intraperitoneal 20 mg/kgBB sekali sehari selam 5 hari, Salep dibuat dengan mencampurkan ekstrak dengan vaselin albumin dan effervescent dibuat dengan mencampurkan ekstrak, dekstrin, asam sitrat, asam tartrat dan natrium bikarbonat. Pengobatan dilakukan selama 10 hari, kemudian dilakukan pengamatan histopatologi kulit. Ekspresi IGF-1 diamati dengan software immunoratio dan jumlah sel MN menggunakan software imageraster. Analisis data menggunakan uji one way ANOVA dan uji tukey dengan tingkat kepercayaan 95%. Hasil menunjukkan terapi oral, salep dan kombinasi berbeda signifikan dibandingkan tikus DM tanpa terapi. Terapi efektif pada terapi kombinasi dimana peningkatan ekspresi IGF-1 hingga 79,20% sedangakan pada jumlah sel MN mengalami penurunan hingga 90,6%. Kesimpulan penelitian ini bahwa ekstrak kulit jeruk manis kombinasi oral dan topikal dapat digunakan sebagai terapi luka insisi penderita DM.
English Abstract
Wounds in DM sufferers result in prolonged inflammatory phases due to high oxidative stress which results in disruption of IGF-1 activity and increasing the cell adhesion of MN in the wound area. Sweet Orange peel is known to contain flavonoids that have antidiabetic and anti-inflammatory properties. This research aimed to know the expression of IGF-1 and the number of MN cells in the incision wound that have been in therapy using the ointment and effervescent extracts of sweet orange peel. The animal models used of rats (Rattus norvegicus) males Wistar strain weight 150-200 gram which were divided into 5 groups with 4 rats each group consist of negative control incision without DM, positive control of the incision of the DM condition, therapy 1 incision of the DM condition treated Effervescent 500 mg/kg BB, therapy 2 treated ointment 5%, Therapy 3 combinations. Therapy was conducted in animal rats DM model streptozotocin induction result through Intraperitoneal 20 mg/kg BB once per day along 5 days, ointment made by extracts mixed with Vaseline albumin and effervescent made by extracts, Dextrin, citric acid, tartrate acid and sodium bicarbonate mixing. Treatment was administreted for 10 days, and then conducted observation of skin histopathology. The expression IGF-1 was measured with ImmunoRatio software and MN cell count used ImageRaster software. Data analysis used one way ANOVA test and Tukey test with 95% confidence rate. The results showed oral therapy, ointment and combination has significantly different of DM rats without therapy. Effective therapy was combination therapy which has elevation of IGF-1 expression up to 79.20% and reduction of the MN cell number to 90.6%. The conclusion of this research were the sweet orange peel extract of oral and topical combinations could be used as a wound therapy for patients with DM
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/26/051908964 |
| Uncontrolled Keywords: | DM, IGF-1, Jumlah sel MN, Tikus putih (Rattus norvegicus), Luka, Kulit Jeruk Manis, Rats (Rattus norvegicus), Wounds, Sweet Orange Peel, DM, IGF-1, the number of mononuclear cells. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 77 Drugs derived from spesific plants (Citrus) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 10 Nov 2020 06:43 |
| Last Modified: | 21 Oct 2021 02:51 |
| URI: | http://repository.ub.ac.id/id/eprint/176720 |
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