Pamungkas, Aulia Fadil (2019) Pengaruh Terapi Ekstrak Etanol Blueberry (Vaccinium corymbosum) terhadap Ekspresi Interleukin-1 Beta dan Jumlah Sel Radang Organ Ginjal Pada Hewan Model Glomerulonefritis Akut Induksi Streptokinase. Sarjana thesis, Universitas Brawijaya.
Abstract
Glomerulonefritis merupakan penyakit ginjal karena adanya inflamasi dan proliferasi sel glomerulus, ditandai oleh peningkatan ekspresi Interleukin 1 beta (IL-1β) dan peningkatan jumlah sel radang. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol Blueberry pada kasus Glomerulonefritis Akut (GNA) terhadap ekspresi Interleukin-1 beta (IL-1β) dan jumlah sel radang. 20 ekor tikus jantan strain wistar yang berumur 6-8 minggu dengan berat badan 150-250 gram dibagi dalam 5 kelompok perlakuan yang terdiri dari kontrol negatif, kontrol positif (diinduksi streptokinase sebanyak 3 kali dosis 6000 IU/ekor dengan interval 5 hari secara IV), perlakuan 1,2,3 diinduksi streptokinase sebanyak 3 kali dosis 6000 IU/ekor dengan interval 5 hari secara IV dan pemberian terapi ekstrak etanol Blueberry dengan dosis masing-masing 500 mg/kg BB, 1000 mg/kg BB, 1500 mg/kg BB selama 14 hari. Setelah 30 hari, ginjal tikus diambil untuk pemeriksaan ekspresi IL-1β menggunakan pewarnaan imunohistokimia dan jumlah sel radang menggunakan pewarnaan hematoksilin eosin, kemudian dianalisa secara statistic menggunakan One Way ANOVA dengan taraf kepercayaan sebesar 95% atau α = 0,05. Hasil penelitian menunjukkan pemberian ekstrak etanol Blueberry dapat menurunkan ekspresi IL-1β dan jumlah sel radang pada organ ginjal dengan dosis terbaik yaitu 1500 mg/kg BB. Kesimpulan dari penelitian ini adalah ekstrak etanol Blueberry memiliki efek terapi terhadap glomerulonefritis akut pada tikus putih yang diinduksi streptokinase berdasarkan ekspresi IL-1β dan jumlah sel radang.
English Abstract
Glomerulonephritis is a kidney disease which is indicated by inflammation and proliferation glomerular cell characterized by an increase in the expression of Interleukin 1 beta (IL-1β) and number of inflammatory cells. The aim of this study is to ascertain the effect of Blueberry ethanol extract on cases of Acute Glomerulonephritis (GNA) on Interleukin-1 beta (IL-1β) expression and increasing of total inflammatory cells. 20 wistar male rats aged 6-8 weeks with weight of 150-250 grams were divided into 5 treatment groups consisting of negative control, positive control (induced by streptokinase 3 times with dose 6000 IU/each with interval 5 days, IV), three groups of treatments induced by streptokinase 3 times with dose 6000 IU/each with interval 5 days and treatments of Blueberry ethanol extract with dose 500 mg/kg BW, 1000 mg/kg BW, 1500 mg/kg BW for 14 days, respectively. After 30 days, rat kidneys were taken for examination of IL-1β expression was using immunohistochemistry and total of inflammatory cells with Hematoxilin-Eosin staining, then analyzed statistically using One Way ANOVA with a confidence level of 95% or α = 0.05. The results showed that the administration of Blueberry ethanol extract as therapy could reduce IL-1β expression and number of inflammatory cells of kidney organ with the best dose of 1500 mg/kg BW. The conclusion of this research is that Blueberry ethanol extract has a therapeutic effect on acute glomerulonephritis that cause by streptokinase based on levels of IL-1β expression and increasing of total inflammatory cells.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/40/051908978 |
| Uncontrolled Keywords: | Blueberry, Glomerulonefritis Akut, Streptokinase, Interleukin 1 beta, Sel Radang, Blueberry, Acute Glomerulonephritis, Streptokinase, Interleukin 1 beta, Inflammatory Cells |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.321 Drugs derived from plants |
| Divisions: | Profesi Kedokteran > Spesialis Dokter Hewan, Fakultas Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 10 Nov 2020 14:11 |
| Last Modified: | 21 Oct 2021 03:05 |
| URI: | http://repository.ub.ac.id/id/eprint/176654 |
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