Hidayat, Guntur Rizal (2019) Pengaruh Pemberian Imunisasi dengan Molekul Adhesin 37,8 kDa Sub Unit Pili Vibrio cholerae terhadap Penurunan Jumlah Koloni Shigella flexneri pada Sekret Usus Mencit Model Diare Shigella flexneri Secara Ex-Vivo. Sarjana thesis, Universitas Brawijaya.
Abstract
Shigellosisvn padavn anakvn menyebabkanvn 29%vn kematianvn anakvn usiavn 1v sampai* 4* tahun*. Imunisasi* pada* shigellosis* sudah* berkembang* dengan* ditemukannya* molekul* adhesin* S. flexneri* dengan* berat* molekul* adesin* 49.8* kDa** dan* molekul* adhesin* 7.9* kda* yang* mempunyai* reaksi* silang* antara* keduanya. Ditemukan* pula* molekul* adhesin* sebesar* 37,8* pada* V. cholare* yang* protektif* terhadap* diare*. Reaksi* silang* molekul* 7,9* kDa* dan* 49,8* kDa* pada* Shigella* flexneri* berpeluang* pula* memiliki* reaksi* silang* dengan* molekul* 37.8* kDa* V. cholera* karena* sifat* dan* bahan* dasar* yang* sama* sehingga* berpeluang* menciptakan* vaksin* homolog* antara* keduanya*. Terdapat* 4* kelompok* sampel* yaitu* kontrol* *negatif, kontrol* positif (*infeksi *S. flexneri* ), *kontrol *imunisasi *dengan *molekul *adhesi *49.8 Kda *S. *flexneri *dan kelompok* perlakuan* imunisasi* molekul* adhesi* 37.8 kDa* V. *cholerae. Berdasarkanwq hasilw analisisw Kruskalw Wallisw dan ujiwpostw hocw Mann-Whitneyw terdapatw penurunanw jumlahw koloni S.felxneri yangw signifikanw dariw kontrolw positifw dibandingw kontrolw negatif. Jumlah koloni wS.flexneri pada wKontrol wpositif k’turun k’signifikan k’dibanding kelompok kontrol kdengan imunisasiK k’49.8 k’kDa k’S.flexneri. Jumlah k’ k’koloni S.flexneri pada kelompok ’ kontol k’ positif k’ turun k’ signifikan k’ dibanding’ perlakuan’ dengan k’ 37.8 kDa k V. cholerae k’. Serta k’ tidak k’ ada k’ perbedaan yang’ bermakna k’ antara k’ kontol k’ negatifdan kedua pemberian imunisasi. Dapat disimpulkan kedua molekul adhesin 37,8 kDa Vibrio cholerae dan 49.8 kDa Shigella flexneri bersifat protektif mencegah Shigellosis pada mencit. Adanya kesamaan sifat tersebut mengindikasikan reaksi silang respon imun antara molekul adhesin Vibrio cholerae dan Shigella flexneri.
English Abstract
Shigellosis in children causes 29% of children deaths aged 1 to 4 years. Immunization in shigellosis has developed with the discovery of the S.flexneri adhesion molecule with molecular weight 49.8 kDa and 7.9 molecule which has a cross reaction. Also found adhesion molecule 37.8 in V. cholare which is protective against diarrhea. Molecular cross reactions of 7.9 kDa and 49.8 kDa in S.flexneri also have potential to have a cross reaction with molecule 37.8 kDa V. cholera because of the same basic propertie so that they have the opportunity to be a homologous vaccine. There are 4 sample groups, negative control, positive control (S. flexneri infection), immunization control with adhesion molecule 49.8 Kda S.flexneri and adhesion molecule immunization 37.8 kDa V. cholerae group. Based on the results of the Kruskal Wallis analysis and the Mann-Whitney post hoc test there was a significant decrease in the number of S. flexneri colonies from positive controls compared to negative controls. The number of S.flexneri colonies in positive controls dropped significantly compared to the control group with immunization of 49.8 kDa S.flexneri. The number of S.flexneri colonies in positive group fell significantly compared to the treatment with 37.8 kDa V. cholerae. And there were no significant differences between negative control and both of immunization. It can be concluded adhesin molecules 37.8 kDa V. cholerae and 49.8 kDa S.flexneri are protective to protect shigellosis in mice. The similarity of these characteristics indicates a cross reaction of the immune response between adhesion molecules V.cholerae and S.flexneri.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/103/051909202 |
| Uncontrolled Keywords: | Shigellosis, molekul adhesi 37.8 V. cholera, molekul adhesi 49.8 kDa S.flexneri. Shigellosis, adhesion molecule 37.8 V. cholera, adhesion molecule 49.8 kDa S.flexneri. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) > 615.107 24 Experimental research |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 19 Jul 2020 07:59 |
| Last Modified: | 19 Oct 2021 06:53 |
| URI: | http://repository.ub.ac.id/id/eprint/176270 |
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