Shidiq, Muhammad Rizal (2019) Pengaruh Pemberian Asam Folat Pre Natal Terhadap Panjang Badan dan Osifikasi Tulang Rawan Larva Zebrafish (Danio rerio) Model Stunting dengan Induksi Rotenon. Sarjana thesis, Universitas Brawijaya.
Abstract
Stunting merupakan gangguan pertumbuhan dan perkembangan yang dialami anak-anak yang diakibatkan oleh nutrisi yang buruk, berulang, dan kurangnya stimulasi psikososial Asam folat merupakan zat yang sangat dibutuhkan untuk pertumbuhan, karena dapat bertindak sebagai anti oksidan dan antiinflamasi. Stunting dapat terjadi apabila terjadi peningkatan stress xoksidatif dari sel sehingga mengganggu pembentukan Growth Hormone (GH) dan Insuline-like Growth Factor-1 (IGF-1). Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian asam folat fase pre natal terhadap panjang badan dan osifikasi tulang rawan pada zebrafish model stunting dengan induksi rotenone. Embrio zebrafish paparan rotenone dan asam folat diberikan mulai usia 0 sampai 72 hpf (hour post fertilization). Hasil analisis statistik menunjukkan pada usia 3, 6 dan 9 dpf (day post fertilization) kelompok perlakuan asam folat dengan konsentrasi 50, 70 dan 100 μM dapat meningkatkan panjang badan secara signifikan jika dibandingkan dengan kelompok kontrol positif (signifikansi p>0,05). Hasil analisis osifikasi tulang rawan yang dilakukan pengukuran pada 9 dpf didapatkan, kelompok kontrol negatif memiliki hasil yang secara signifikan lebih rendah jika dibandingkan dengan kelompok kontrol positif (p<0.05). Dan perbandingan antara kelompok kontrol positif dengan kelompok perlakuan asam folat konsentrasi 50 μM tidak didapatkan adanya perbedaan yang signifikan (p>0.05). Asam folat konsentrasi 70 μM dan 100 μM secara signifikan dapat meningkatkan osifikasi tulang rawan jika dibandingkan dengan kelompok kontrol positif (p<0.05). Perbandingan antara kelompok kontrol negatif dengan kelompok perlakuan asam folat konsentrasi 70 μM tidak didapatkan perbedaan yang signifikan (p>0.05), sehingga dapat disimpulkan bahwa kelompok perlakuan asam folat konsentrasi 70 μM memberikan efek yang mendekati kelompok kontrol.
English Abstract
Stunting is a growth and developmental problem which occurred on childred that is mainly caused by bad nutritional feeding, recurring infections, and an overall lack of psychosocial stimulation. Folic Acid is a highly needed substance for growth, because it can act as an antioxidant agent and as an anti inflammatory agent. Stunting can occur whenever there is an increase in oxidative stress of the cell which in turn disrupts the production of Growth Hormone (GH) and Insuline-like Growth Factor (IGF-1). The purpose of this research is to explore he effect of pre natal folic acid administration on pre-natal phase to body length and catillage ossification in stunted zebrafish model with rotenone induction where the zebrafish’s embryos were given rotenone exposure frome the age of 0 until 72 hpf (hour post fertilization). The administration of folic acid were given in the same time range so the prevention effect could be observed. The results of statistical analysis showed that at the age of 3, 6 and 9 dpf (day post fertilization) the treatment group of folic acid with a concentration of 50, 70 and 100 μM could significantly increase the length of the body when compared to the positive control group (significance p>0.05). The results of cartilage ossification analysis performed at 9 dpf which were obtained showed that the negative control group had significantly lower results compared to the positive control group (p <0.05). And the comparison between the positive control group and the group of folic acid treatment with a concentration of 50 μM was not found to be significantly different (p>0.05). Folic acid concentration of 70 μM and 100 μM significantly increased the cartilage ossification when compared to the positive control group (p<0.05). The comparison between the negative control group and the folic acid treatment group with a concentration of 70 μM did not show a significant difference (p>0.05), so it could be concluded that the treatment group of 70 μM folic acid had an effect that approached the control group.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/301/051909425 |
| Uncontrolled Keywords: | stunting, asam folat, rotenon, osifikasi, zebrafish, stunting, folic acid, rotenone, ossification, zebrafish. |
| Subjects: | 600 Technology (Applied sciences) > 612 Human Physiology > 612.6 Reproduction, development, maturation > 612.65 Child development |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 26 Aug 2020 04:38 |
| Last Modified: | 25 Oct 2021 02:18 |
| URI: | http://repository.ub.ac.id/id/eprint/176175 |
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