Naprilia, Flora Nunjil (2019) Pengaruh Pemberian Ekstrak Etanol Brokoli (Brassica oleracea) Terhadap Jumlah Folikel Ovarium Tikus Putih (Rattus norvegicus) Galur Wistar yang Dipapar Monosodium Glutamat (MSG). Sarjana thesis, Universitas Brawijaya.
Abstract
Penggunaan monosodium glutamat (MSG) sebagai penyedap rasa semakin dikenal pada masyarakat dan meningkatkan setiap tahunnya, Mengkonsumsi monosodium glutamat yang berlebihan akan menstimulasi pembentukan radikal bebas yang berbahaya bagi kesehatan reproduksi, khususnya pada wanita dapat menimbulkan gangguan folikulogenesis ovarium. Efek radikal bebas dalam tubuh dapat dicegah dengan pemberian antioksidan flavonoid tertinggi dari sepuluh sayuran yang diteliti. Tujuan penelitian ini adalah untuk membuktikan pengaruh pemberian ekstrak etanol brokoli (Brassica oleracea) terhadap peningkatan jumlah folikel ovarium yang dipapar MSG. Metode yang digunakan dengan rancangan Randomized Post Test Only untuk membandingkan jumlah folikel ovarium antar kelompok selama 35 hari (7 hari adaptasi dan 28 hari perlakuan) dengan sampel 30 ekor tikus dan dibagi 5 kelompok, yaitu K(-) tidak dipapar MSG maupun ekstrak etanol brokoli; K(+) dipapar MSG 0,7 mg/gBB dan tidak diberi ekstrak etanol brokoli; perlakuan yaitu: (P(1) MSG 0,7 mg/gBB dan diberi ekstrak etanol brokoli 500 mg/kgBB), (P(2) MSG 0,7 mg/gBB dan diberi ekstrak etanol brokoli 1000 mg/kgBB), (P(3) MSG 0,7 mg/gBB dan diberi ekstrak etanol brokoli 2000 mg/kgBB). Pada hari ke-29 dilakukan terminasi pada tikus yang mengalami fase proestrus. Data dianalisis dengan ANOVA, dan Post Hoc Tukey. Hasil penelitian ini menunjukkan p<0,05 sehingga terdapat pengaruh pemberian ekstrak etanol brokoli terhadap jumlah folikel ovarium, jumlah folikel ovarium (P1, P2, P3) mampu meningkatkan secara bermakna dibandingkan K(+), serta dosis efektif pemberian ekstrak etanol brokoli yang mampu meningkatkan jumlah folikel ovarium adalah dosis 2000 mg/KgBB. Dapat disimpulkan bahwa untuk pemberian ekstrak etanol brokoli mampu meningkatkan jumlah folikel ovarium tikus putih yang dipapar monosodium glutamat.
English Abstract
The use of monosodium glutamate (MSG) as a flavoring are known to the public and increases every year. Consuming excessive monosodium glutamate will stimulate the formation of free radicals that are harmful to reproductive health, especially in women can cause ovarian folliculogenesis disorders. The effects of free radicals in the body can be prevented by giving the highest amount of flavonoid antioxidants from the ten vegetables it has been studied. The purpose of this study was to prove the effect of giving extract of ethanol broccoli (Brassica oleracea) to increase the number of ovarian follicles exposed to MSG. The method used was the design of Randomized Test Only to compare the number of ovarian follicles between groups for 35 days (7 days of adaptation and 28 days of treatment) with a sample of 30 rats and divided into 5 groups, namely K(-) not exposed to MSG or extract ethanol broccoli ; K(+) exposed to 0.7 mg/gBB MSG and not given extract broccoli ethanol; the treatments were: (P(1) 0.7 mg/gBB MSG and extract broccoli ethanol 500 mg/kgBB), (P(2) 0.7 mg/gBB MSG and extrac broccoli ethanol t 1000 mg/kgBB), (P(3) MSG 0.7 mg/gBB and given broccoli ethanol extract of 2000 mg/kgBB. On the 29th day termination was carried out in rats undergoing the proestrus phase. Data were analyzed by ANOVA, and Post Hoc Tukey. The results of this study showed p <0.05 so that there was an effect of giving broccoli ethanol extract to the number of ovarian follicles, the number of ovarian follicles (P1, P2, P3) able to increase significantly compared to K(+), and the effective of doses extract broccoli ethanol capable increasing the number of ovarian follicles is a dose of 2000 mg/kgBB. It can be concluded that the administration of extract broccoli ethanol can increase the number of ovarian follicles of white rats exposed to monosodium glutamate.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/97/051909197 |
| Uncontrolled Keywords: | Ekstrak etanol brokoli, MSG, jumlah folikel ovarium, tikus putih, broccoli ethanol extract, MSG, number of ovarian follicles, white mice |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica) > 615.107 24 Experimental research |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 19 Jul 2020 04:12 |
| Last Modified: | 11 Mar 2022 06:41 |
| URI: | http://repository.ub.ac.id/id/eprint/175222 |
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