Maslukah, Ruwiyatul (2019) Pengaruh Polyethylene Glycol (Peg) Terhadap Hardening Planlet Apel (Malus Sp.) Akibat Hiperhidrisitas Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Apel (Malus sp.) ialah salah satu jenis tanaman buah yang memiliki nilai ekonomi tinggi di seluruh dunia. Produksi apel di Indonesia sangat rendah, karena keterbatasan daerah yang sesuai untuk budidaya apel, selain itu penyedian bibit juga belum terlalu banyak. Perbanyakan dengan kultur jaringan sudah banyak dilakukan oleh para peneliti, namun masih terdapat kendala yaitu terjadi hiperhidrisitas pada planlet apel. Kendala tersebut telah mengakibatkan terjadinya kegagalan aklimatisasi, oleh karena itu ketersedian bibit batang bawah apel belum dapat terpenuhi. Tujuan dari penelitian ini ialah untuk mengetahui pengaruh penggunaan polyethylene glycol (PEG) 6000 dalam mempercepat hardening plantlet apel akibat hiperhidrisitas dan mendapatkan konsentrasi PEG yang optimal dalam mengatasi hiperhidrisitas pada plantlet apel. Penelitian dilakukan di Laboratorium Kultur Jaringan BALITJESTRO yang dilaksanakan pada bulan April-Juli 2018. Peralatan yang digunakan antara lain, botol kultur, oven, kompor, panci, pengaduk, gelas ukur, beaker glass, spatula, pH meter, magnetik stirrer, timbangan analitik, autoclave, pinset, skalpel, gunting, bunsen, LAF, plastik wrap, solatip, spidol OHP, cawan petri, handsprayer, camera, penggaris, dan ruang kultur (rak, lampu neon, AC, thermometer). Bahan yang digunakan ialah, plantlet apel hiperhidrisitas, media kultur (Murashinge and Skoog), vitamin, myo-inositol, malt extract, aquades, gula, agar-agar, kertas hvs, alkohol 96%, alkohol 70%, spiritus, kertas millimeter blok dan PEG 6000. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan masing-masing perlakuan antara lain, PEG 0 g/l, PEG 1 g/l, PEG 3 g/l, PEG 5 g/l, PEG 7 g/l dan PEG 9 g/l dengan lima kali ulangan. Pengamatan dilakukan selama enam kali dalam interval waktu dua minggu sekali. Variabel yang diamati meliputi: tinggi plantlet, jumlah daun, jumlah tunas, jumlah ruas, bobot segar akhir, % planlet hidup, dan warna daun. Data yang diperoleh dianalisis menggunakan analisis ragam (uji F) pada taraf 5% untuk mengetahui pengaruh perlakuan. Jika analisis ragam nyata dilakukan uji lanjut dengan menggunakan uji Beda Nyata Terkecil (BNT) pada taraf 5% untuk mengetahui perbedaan diantara perlakuan. Hasil penelitian pada pemberian konsentrasi PEG 1 g/l sampai dengan 5 g/l nyata terhadap pertumbuhan planlet berdasarkan variabel pengamatan tinggi planlet, jumlah tunas dan jumlah ruas. Sedangkan pemberian konsentrasi lebih tinggi menurunkan pertumbuhan planlet. Pemberian konsentrasi PEG berpengaruh terhadap tingkat percepatan hardening planlet apel akibat hiperhidrisitas dan Pemberian konsentrasi PEG 5 g/l merupakan konsentrasi yang optimum untuk mengatasi hiperhidrisitas pada planlet apel
English Abstract
Apple (Malus sp.) is one of the most economically important fruits worldwide. In Indonesia, production of apple fruit is low, because of limited area suitable for apple cultivation, besides the supply of seeds is not too much. Propagation with tissue culture has been done by many researchers, but there are having problem, this problem is hyperhidricity in apple planlets. This problem has been giving stress on acclimatization, therefore the availability of apple rootstock seedlings has not been failure. The purpose of this study was to determine the effect of polyethylene glycol (PEG) 6000 to fast hardening of apple plantlet by the effect of hyperhydricity and to get the optimal concentration of PEG to get over hyperhydricity in apple plantlet. The research was conducted at BALITJESTRO which was held in AprilJuly 2018. The equipment to be used included culture bottles, ovens, stoves, pans, stirrers, measuring cups, beaker glass, spatula, pH meters, magnetic stirrers, analytical scales, autoclave, tweezers, scalpel, scissors, bunsen, LAF, plastic wrap, solution, OHP marker, petri dish, handsprayer, camera, ruler, and culture room (shelves, neon lights, AC, thermometer). The materials used are, apple plant hyperhydricity, culture media (Murashinge and Skoog), vitamins, myo-inositol, malt extract, aquades, sugar, agar, HVS paper, 96% alcohol, 70% alcohol, spirits, millimeter block paper and PEG 6000. This study used a completely randomized design (CRD) with each treatment by the others, PEG 0, PEG 1 g/l, PEG 3 g/l, PEG 5 g/l, PEG 7 g/l and PEG 9 g/l, with five replications. Observations are made six times at intervals of two weeks. The variables observed included: plantlet high, number of leaves, number of shoots, number of internodes, wet weight fresh, % live planlets, and leaf color. The data obtained were analyzed using variance analysis (F test) at the level of 5% to determine the effect of treatment. If the analysis of the real variance is carried out further testing using the least significant differences (LSD) at the level of 5% to find out the difference between treatments. The results of the study by used to concentrations of PEG 1 g/l to 5 g/l were significant different on plantlet growth based on observation variables of plantlets height, number of shoots and number of internodes. While giving higher concentration decreases on plantlet growth. Apply concentration of PEG has been effected on the rate of hardening apple plantlets by the effect of hyperhydricity and adding of concentration 5 g/l PEG is the optimum concentration to solving hyperhydricity on apple planlet.
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2019/684/05197458 |
| Subjects: | 600 Technology (Applied sciences) > 634 Orchards, fruits, forestry > 634.1 Pomaceous fruits > 634.11 Apples |
| Divisions: | Fakultas Pertanian > Budidaya Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 24 Aug 2020 07:24 |
| Last Modified: | 24 Aug 2020 07:24 |
| URI: | http://repository.ub.ac.id/id/eprint/173821 |
Actions (login required)
 |
View Item |