Ketrina, Sitiani (2019) Kelimpahan dan Keanekaragaman Bakteri Rizosfer pada Lahan Tanaman Wortel dengan Penggunaan Fungisida Berbahan Aktif Propineb dan Lahan Tana-man Wortel Organik. Sarjana thesis, Universitas Brawijaya.
Abstract
Produksi tanaman wortel nasional cenderung menurun dari tahun ke tahun. Upaya yang dilakukan oleh petani untuk meningkatkan produksi wortel salah satunya dengan cara pengendalian terhadap penyakit pada tanaman wortel secara kimiawi (pengaplikasian fungisida propineb). Hal tersebut berdampak pada penurunan kualitas dari biologi tanah. Salah satu indikator sifat biologi tanah yaitu biodiversitas tanah. Mikroba tanah sebagai bagian dari biodiversitas tanah memiliki banyak peran penting dalam tanah. Bakteri merupakan jenis mikroba tanah yang paling dominan, populasi dari bakteri mencapai separuh dari biomassa mikroba da-lam tanah. Faktor yang mempengaruhi kelimpahan dan keanekaragaman bakteri rizosfer salah satunya yaitu input kimiawi. Tujuan penelitian ini mengkaji kelimpahan dan keanekaragaman bakteri rizosfer di lahan organik dan lahan konvensional yang diaplikasikan fungisida berbahan aktif Propineb dan mengetahui daya tahan bakteri terhadap fungisida berbahan aktif Propineb. Penelitian dilaksanakan mulai pada bulan Januari hingga Mei 2019 di Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya. Metode pelaksanaan penelitian yang dilakukan meliputi survei, pengambilan sampel tanah di lahan tanaman wortel Agrotechno Park Cangar Universitas Brawijaya dan Desa Sumber Brantas Batu, isolasi bakteri rizosfer, identifikasi, uji hipersensitif dan uji kemampuan hidup bakteri rizosfer pada media NA yang dicampur dengan fungisida berbahan aktif propineb. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari lima perlakuan yakni 0,5 gr/l, 1,0 gr/l, 1,5 gr/l, 2,0 gr/l, 2,5 gr/l dan masing-masing perlakuan diulang sebanyak tiga kali. Hasil penelitian menunjukkan bahwa total isolat bakteri rizosfer yang ditemukan pada lahan konvensional sebanyak 22 isolat, dimana 64% dari total iso-lat bakteri rizosfer bersifat nonpatogen. Pada lahan organik ditemukan sebanyak 38 isolat dengan persentase bakteri rizosfer yang bersifat nonpatogen sebesar 77%. Berdasarkan hasil perhitungan keanekaragaman bakteri rizosfer, diperoleh hasil yang menunjukkan bahwa lahan organik memiliki tingkat keanekaragaman bakteri rizosfer lebih tinggi dibandingkan lahan konvensional yaitu sebesar H’= 3,29 (tinggi) dan H’= 2,52 (sedang). Total kelimpahan bakteri rizosfer yang ditemukan pada lahan organik lebih tinggi dibandingkan lahan konvensional yaitu 51,7 x 1012 cfu/g dan 15,6 x 1012 cfu/g. Hasil identifikasi bakteri rizosfer yang dominan pada lahan konvensional yaitu Erwinia sp. (KE9) bersifat patogen, Clostridium sp. (KA10) bersifat non patogen dan Pantoea sp. (KA11) bersifat patogen sedangkan bakteri rizosfer yang dominan pada lahan organik yaitu Pseudomonas sp. (OG9), Bacillus sp. (OL10) dan Erwinia sp. (OF11) bersifat non patogen. Hasil pengujian ketahanan bakteri rizosfer dominan dari kedua lahan terhadap fungisida berbahan aktif propineb pada konsentrasi 0,5 gr/l hingga 2,5 gr/l menunjukkan bahwa keenam isolat tersebut mampu tumbuh pada media NA yang diinkubasi selama 48 jam.
English Abstract
The production of national carrot plants tends to decline from year to year. Efforts made by farmers to increase carrot production, one of them by controlling the disease in carrot plants chemically (application of propineb fungicides). This results in a decrease in the quality of soil biology. One indicator of soil biological properties is soil biodiversity. Soil microbes as part of soil biodiversity have many important roles in the soil. Bacteria are the most dominant type of soil microbes, the population of bacteria reaches half of microbial biomass in the soil. One of the factors that influence the abundance and diversity of rhizosphere bacteria is chem-ical input. The purpose of this study was to examine the abundance and diversity of rhizosphere bacteria in organic land and conventional land and to know the re-sistance of bacteria to fungicides made from Propineb. The research was conducted from January to May 2019 at the Laboratory of Plant Diseases, Faculty of Agriculture, Universitas Brawijaya. Methods of conduct-ing research include surveys, soil sampling in the carrots of Agrotechno Park Cangar, Brawijaya University and Sumber Brantas Batu Village, isolation of rhizo-sphere bacteria, identification, hypersensitivity test and test of living ability of rhi-zosphere bacteria on NA media mixedwith fungicides using active propineb. This study used a completely randomized design (CRD) consisting of five treatments namely 0.5 gr / l, 1.0 gr / l, 1.5 gr / l, 2.0 gr / l and 2.5 gr / l and each treatment was repeated three times. The results showed that the total isolates of rhizosphere bacteria found in con-ventional land were 22 isolates, of which 64% of the total isolates of the rhizosphere bacteria were non-pathogenic. 38 isolates were found on organic land with 77% of the rhizosphere bacteria were nonpatogen. Based on the results of the calculation of the diversity of rhizosphere bacteria, the results showed that organic land has a higher level of diversity of rhizosphere bacteria than conventional land, which is equal to H '= 3.29 (high) and H' = 2.52 (medium). The total abundance of rhizo-sphere bacteria found in organic land was higher than conventional land which was 51.7 x 1012 cfu / g and 15.6 x 1012 cfu / g. The results of identification of the dominant rhizosphere bacteria on conventional land, Erwinia sp. (KE9) is patho-genic, Clostridium sp. (KA10) is non-pathogenic and Pantoea sp. (KA11) are path-ogenic while the rhizosphere bacteria which are dominant in organic land are Pseu-domonas sp. (OG9), Bacillus sp. (OL10) and Erwinia sp. (OF11) is non pathogenic. The results of testing the resistance of the dominant rhizosphere bacteria from the two fields to the fungicides made from propineb showed that the six isolates were said to be able to grow on NA media which was incubated for 48 hours.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2019/528/051907302 |
| Uncontrolled Keywords: | - |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control > 632.95 Pesticides > 632.952 Fungicides |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 24 Aug 2020 07:18 |
| Last Modified: | 10 Jan 2022 02:54 |
| URI: | http://repository.ub.ac.id/id/eprint/173610 |
Preview |
Text
Sitiani Ketrina.pdf Download (3MB) | Preview |
Actions (login required)
 |
View Item |