Pratiwi, Puji (2019) Potensi Bakteri Rizosfer Sebagai Bioremediator Pada Lahan Tercemar Herbisida Glifosat Dan Agens Antagonis Dalam Menghambat Penyakit Rebah Semai Pada Tanaman Kedelai. Sarjana thesis, Universitas Brawijaya.
Abstract
Kedelai adalah salah satu sumber pangan utama yang dikonsumsi penduduk di Indonesia. Namun produksi tanaman kedelai belum bisa memenuhi kebutuhan konsumsinya. Rendahnya produksi kedelai dapat diakibatkan oleh faktor kesehatan. Upaya alternatif yang dapat digunakan untuk menanggulangi turunnya produksi kedelai akibat faktor kesehatan ialah dengan bioremediasi dan pemanfaatan agens antagonis. Berdasarkan hasil penelitian sebelumnya diperoleh bakteri dari rizosfer tanaman wortel, pinus, dan kopi di UB forest. Penelitian bertujuan untuk mengkaji potensi bakteri rizosfer sebagai bioremediator pada tanah yang tercemar herbisida glifosat dan agens antagonis dalam menghambat penyakit rebah semai pada tanaman kedelai. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan, Laboratorium Toksikologi Pestisida, dan Rumah Kawat Jurusan Hama Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya dari Januari 2019 – Juni 2019. Tahapan penelitian meliputi seleksi bakteri rizosfer toleran herbisida glifosat secara in vitro, uji antagonisme bakteri toleran terhadap jamur patogen S. rolfsii, uji potensi bakteri toleran herbisida di pertanaman kedelai terhadap pertumbuhan tanaman kedelai dan terhadap penekanan kejadian penyakit rebah semai di Rumah Kawat, dan uji residu herbisida glifosat pada tanah oleh bakteri toleran herbisida glifosat. Hasil seleksi 10 bakteri rizosfer pada medium mengandung herbisida glifosat 2ml/l diketahui bahwa semua isolat bakteri yang digunakan mampu tumbuh pada 48 JSI. Uji penghambatan terhadap jamur diketahui terdapat 6 isolat bakteri yang mampu menghambat patogen S. rolfsii, ke-6 isolat tersebut ialah N3 (Bacillus sp.), N10 (Clostridium sp.), N23 (Xanthomonas sp.), N24 (Pseudomonas sp.), N26 (Pantoea sp.), N27 (Erwinia sp.). Uji penekanan penyakit rebah semai diketahui bahwa ke 6 isolat bakteri N3 (Bacillus sp.), N10 (Clostridium sp.), N23 (Xanthomonas sp.), N24 (Pseudomonas sp.), N26 (Pantoea sp.), N27 (Erwinia sp.) mampu menekan kejadian penyakit rebah semai pada tanaman kedelai. Uji potensi terhadap pertumbuhan tanaman kedelai di rumah kawat diketahui bahwa isolat bakteri N3 (Bacillus sp.) mampu meningkatkan pertumbuhan tanaman kedelai (tinggi tanaman, panjang akar dan jumlah daun) dibandingkan dengan kontrol serta isolat N24 (Pseudomonas sp.) mampu meningkatkan pertumbuhan tanaman kedelai (tinggi tanaman dan panjang akar) dibandingkan dengan kontrol. Isolat N3 (Bacillus sp.) dan N10 (Clostridium sp.) diketahui secara signifikan mampu menurunkan residu herbisida glifosat di tanah pada 70 hsa dengan metode HPLC
English Abstract
Soybean is one of the main food sources consumed by people in Indonesia. But the production of soybean plants has not been able to fulfill consumption needs. Low soybean production can be caused by health factors. Alternative efforts that can be used to overcome the decline in soybean production due to health factors are by bioremediation and utilization of antagonistic agents. Based on the results of previous studies obtained by bacteria from rhizosphere of carrot, pine and coffee plants in UB forest. The aim of the study was to examine the potential of rhizosphere bacteria as bioremediators in soil contaminated with herbicide glyphosate and antagonistic agents in inhibiting dumping off on soybean plants. The research was held at the Laboratory of Plant Disease, Pesticide Toxicology Laboratory, and Green House of the Department Plant Disease, Faculty of Agriculture, Brawijaya University from January 2019 - May 2019. The research stages included selection of glyphosate-tolerant rhizosphere bacteria in vitro, bacterial antagonism tolerant to S. rolfsii, test the potential of herbicide tolerant bacteria in soybean plantations on soybean plant growth and on suppressing the incidence of dumping off disease in the Green House, and herbicide residue testing of glyphosate on soil by herbicide tolerant bacteria glyphosate. The results of the selection of 10 rhizosphere bacteria in the medium containing glyphosate 2ml / l herbicide found that all bacterial isolates used were able to grow at 48 JSI. The inhibitory test on fungi found that there were 6 bacterial isolates that were able to inhibit the pathogen S. rolfsii, the 6 isolates were N3 (Bacillus sp.), N10 (Clostridium sp.), N23 (Xanthomonas sp.), N24 (Pseudomonas sp.) , N26 (Pantoea sp.), N27 (Erwinia sp.). Test of suppression of dumping off disease is known that the 6 isolates of N3 (Bacillus sp.), N10 (Clostridium sp.), N23 (Xanthomonas sp.), N24 (Pseudomonas sp.), N26 (Pantoea sp.), N27 (Erwinia sp .) able to suppress the incidence of dumping off on soybean plants. Potential test on the growth of soybean in green house is known that isolates of N3 bacteria (Bacillus sp.) were able to increase the growth of soybean plants (plant height, root length and number of leaves) compared to controls and N24 (Pseudomonas sp.) Isolates can increase soybean plant growth (plant height and root length) compared to controls. N3 (Bacillus sp.) And N10 (Clostridium sp.) Isolates were found to be significantly able to reduce glyphosate in soil residue herbicide in 70 hsa by HPLC method.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2019/99/051906816 |
| Subjects: | 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.3 Legumes, forage crops other than grasses and legumes > 633.34 Soybeans |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 18 Aug 2020 03:02 |
| Last Modified: | 21 Oct 2021 06:59 |
| URI: | http://repository.ub.ac.id/id/eprint/173447 |
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