Sari, Novita (2019) Potensi Bakteri Antagonis Pada Limbah Cair Tahu Untuk Menghambat Bakteri Xanthomonas Axonopodis Pv. Glycines Penyebab Penyakit Pustul Bakteri Pada Kedelai (Glycine Max L.). Sarjana thesis, Universitas Brawijaya.
Abstract
Kedelai (Glycine max L.) merupakan salah satu tanaman palawija di Indonesia. Kedelai merupakan salah satu bahan baku utama pembuatan tahu. Proses produksi tahu di Indonesia masih menggunakan cara tradisional sehingga menghasilkan limbah cair. Beberapa penelitian membuktikan bahwa pada limbah cair tahu mengandung bahan organik tinggi dan diduga terdapat mikroorganisme yaitu bakteri yang dapat mengendalikan patogen tanaman. Tujuan dari penelitian ini yaitu untuk mengkaji bakteri antagonis pada limbah cair tahu dalam mengendalikan patogen X. a. pv. glycines penyebab penyakit pustul bakteri pada kedelai secara in vitro. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya Malang mulai bulan Desember 2018 hingga bulan Mei 2019. Tahapan penelitian meliputi isolasi dan identifikasi bakteri penyebab penyakit pustul bakteri, eksplorasi dan seleksi bakteri limbah cair tahu, uji hipersensitif bakteri antagonis limbah cair tahu, uji penghambatan bakteri antagonis terhadap X. a. pv. glycines secara in vitro. Uji penghambatan menggunakan Rancangan Acak Lengkap (RAL) 8 perlakuan dan diulang sebanyak 4 kali, serta karakterisasi dan identifikasi bakteri sampai tingkat genus. Hasil eksplorasi diperoleh 60 isolat bakteri limbah cair tahu dengan 33 isolat bakteri yang memiliki kemampuan antagonis pada X.a. pv. glycines. Dari 33 isolat bakteri dipilih 6 bakteri terbaik yaitu isolat bakteri dengan kode A1, A2, A15, C8, D7, dan D9 untuk uji selanjutnya. Hasil uji antagonis menunjukkan bahwa 6 isolat bakteri limbah cair tahu terpilih mampu menghambat pertumbuhan bakteri X.a pv. glycines. Hasil analisis ragam menunjukkan antar perlakuan memberikan pengaruh nyata terhadap hasil rerata diameter zona hambat terhadap bakteri X.a. pv. glycines. Rerata diameter zona hambat yang tertinggi yaitu pada isolat C8, D7, dan D9 sedangkan hasil terendah yaitu pada isolat A1, A2, dan A15. Hasil rerata diameter zona hambat keenam isolat bakteri berbeda nyata terhadap kontrol Streptomicin. Hasil rerata diameter zona hambat isolat A1, A2, dan A15 tidak berbeda nyata terhadap kontrol akuades steril. Berdasarkan hasil karakterisasi dan identifikasi terhadap bakteri antagonis diketahui bahwa isolat A1, D7, dan D9 termasuk ke dalam genus Pantoea. Isolat bakteri A2 dan A15 termasuk ke dalam genus Clostridium. Isolat bakteri C8 termasuk ke dalam genus Erwinia.
English Abstract
Soybean (Glycine max L.) is one of the secondary crops in Indonesia. Soybean is one of the raw materials for making tofu. Most of tofu production in Indonesia is using traditional methods to produce liquid waste. Some studies have found that in tofu liquid waste can produce bacteria that can control plant pathogens. Therefore, it is to do a study related to the potential of antagonistic bacteria in tofu liquid waste. The purpose of this study antagonistic bacteria in tofu liquid waste to inhibit pathogen X. a. pv. glycines bacterial caused pustule disease in vitro. The research was conducted at the Laboratory of Plant Diseases, Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University Malang, starting in December 2018 until May 2019. The stages of the study included isolation and identification of bacteria causing bacterial pustules, exploration and selection of tofu liquid waste bacteria, hypersensitivity of antagonistic bacteria in tofu liquid waste, test the inhibition of antagonistic bacteria against X. axonopodis pv. glycines in vitro using a Randomized Complete Design (RAL) 7 treatments and repeated 4 times, as well as characterization and identification antagonistic bacteria to the genus level. The exploration results obtained 60 isolates of tofu liquid waste bacteria with 33 bacterial isolates that had antagonistic abilities at X.a. pv. glycines. From the 33 bacterial isolates, 6 of the best bacteria were selected, namely bacterial isolates with codes A1, A2, A15, C8, D7, and D9 for further testing. The antagonistic test results showed that 6 isolates bacteria were able to inhibit the growth of bacteria X.a. pv. glycines. The results of the variance analysis showed that between treatments gave a real effect on the results of the average clear zone diameter of bacteria X.a. pv. glycines. From the six selected bacterial isolates, the highest clear zone diameter results were obtained, were in isolates C8, D7, and D9 while the lowest results were in isolates A1, A2, and A15. The results of the average diameter of clear zone of the six bacterial isolates were significantly different from the control of Streptomycin. The results of the average diameter of clear zone isolates A1, A2, and A15 were not significantly different from the control of sterilized aquades. Based on the results of characterization and identification of antagonistic bacteria it is known that isolates A1, D7, and D9 belong to the genus Pantoea. The bacterial isolates A2 and A15 belong to the genus Clostridium. C8 bacterial isolates belong to the genus Erwinia.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2019/109/051906826 |
| Subjects: | 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.3 Legumes, forage crops other than grasses and legumes > 633.34 Soybeans |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 18 Aug 2020 03:02 |
| Last Modified: | 21 Oct 2021 07:23 |
| URI: | http://repository.ub.ac.id/id/eprint/173419 |
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