Fauzi, Hadyan Taufiq Nur (2019) Verifikasi F1 Kedelai Hasil Persilangan Varietas Grobogan Dan Biosoy 1 Dengan Kedelai Introduksi Menggunakan Marka Ssr. Sarjana thesis, Universitas Brawijaya.
Abstract
Kedelai (Glycine max (L.) Merr.) ialah salah satu komoditas pangan yang dikonsumsi oleh masyarakat Indonesia, selain padi dan jagung. Konsumsi kedelai nasional cukup tinggi, sedangkan produksi kedelai dalam negeri sangat rendah. Salah satu upaya yang dilakukan Pemerintah untuk mengurangi ketergantungan impor ialah dengan cara ekstensifikasi lahan pertanian. Akan tetapi, perluasan lahan dihadapkan pada tantangan besar yaitu tidak tersedianya lagi lahan-lahan optimal. Sehingga, ekstensifikasi lahan pertanian lebih difokuskan pada lahanlahan suboptimal. Oleh karena itu, diperlukan adanya varietas-varietas kedelai mampu berproduksi dengan optimal meskipun ditanam pada lahan yang kurang optimal. Dalam penyediaan varietas kedelai baru, upaya yang dapat dilakukan salah satunya ialah kegiatan pemuliaan tanaman. Kegiatan pemuliaan tanaman dengan dukungan marka molekuler diharapkan dapat mempercepat proses seleksi karena marka molekuler mampu mendeteksi gen target tanpa dipengaruhi oleh lingkungan, sehingga kegiatan pemuliaan tanaman menjadi lebih efektif. Tujuan dari penelitian ini ialah untuk memverifikasi keberhasilan persilangan pada tanaman kedelai F1 putatif menggunakan marka SSR. Penelitian dilaksanakan pada bulan November 2018 hingga April 2019 di Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian Bogor. Bahan yang digunakan dalam penelitian ini ialah kedelai varietas Grobogan, Biosoy 1, dan aksesi introduksi asal Amerika Serikat (PI 553045, PI 471938 dan PI 471931), tanah, pupuk organik kotoran sapi “Karyana”, kapur dolomit, pupuk NPK 16-16-20, gandasil D, gandasil B, insektisida Decis, kertas label, benang, sampel daun segar, PVP 2%, CTAB (Cetyl Trimethyl Ammonium Bromide), natrium bisulfit 0,38%, natrium asetat (NaOAc) 3M, kloroform isoamilalkohol 24:1, isopropanol, etanol 70%, buffer Tris-EDTA (TE) 1x pH 8.0, KAPA2G Fast ReadyMix PCR Kit, ddH2O, gel agarose, buffer Tris Asetat EDTA (TAE) 1x pH 8.0, SYBR Gold Nucleic Acid Gel Stain, 1 kb DNA Ladder, molecular water (MW), 15 primer SATT untuk SSR-PCR, akrilamidbisakrilamid 8%, Ammonium Persulfate (APS) 10%, TEMED, Mix electrophoresis, marker 1 kb, buffer Tris Borate EDTA (TBE) 1x pH 8.0, tube 1.5 ml, tube 2 ml, dan tisu KimWipes. Sedangkan alat yang digunakan ialah pot plastik 10 kg, sprayer, pinset, blue pestle, erlenmeyer, gelas ukur, mikropipet, tips, inkubator, water bath, sentrifuge, tube rack, lemari pendingin, Speedvac DNA Concentrator, 96-plate tube, T1 Thermocycler (PCR), plate gel agarose, plate gel poliakrilamid, elektroforesis vertikal, elektroforesis horizontal, baki, dan UV Transilluminator. Metode penelitian ini terdiri dari penanaman tetua, persilangan, penanaman F1, isolasi DNA, uji kualitatif dan kuantitatif DNA, PCRSSR, elektroforesis gel poliakrilamid, dan visualisasi hasil elektroforesis. Variabel pengamatan terdiri atas jumlah bunga yang disilangkan, jumlah polong yangterbentuk, persentase keberhasilan persilangan, dan keberhasilan persilangan dari hasil analisis molekuler. Analisis data dilakukan dengan Analisis Deskriptif. Hasil penelitian menunjukkan bahwa verifikasi persilangan tanaman menggunakan 5 pasang primer terpilih menunjukkan dari 65 tanaman F1 putatif yang diuji, sebanyak 15 tanaman terverifikasi merupakan true F1 karena memiliki pita DNA yang berasal dari gabungan pita DNA kedua tetua. F1 positif terbanyak terdapat pada populasi Grobogan x Introduksi 12 dan Grobogan x Introduksi 13, yaitu sebanyak 5 tanaman. Sedangkan F1 positif paling sedikit terdapat pada populasi Biosoy 1 x Introduksi 10, yaitu sebanyak 2 tanaman F1
English Abstract
Soybean (Glycine max (L.) Merr.) is one of the food commodities consumed by Indonesian people, besides rice and corn. National soybean consumption is quite high, while domestic soybean production is very low. One of the efforts made by the Government to reduce import dependence is by extending agricultural land. However, land expansion is faced with a major challenge, which is the unavailability of optimal land. Thus, the extensification of agricultural land is more focused on suboptimal lands. Therefore, there is a need for soybean varieties that able to produce optimally even though it is planted on suboptimal land. To supply new soybean varieties, one of the efforts that can be done is plant breeding activities. Plant breeding activities with molecular marker support are expected to accelerate the selection process because molecular markers can detect target genes without being influenced by the environment, so that plant breeding activities are more effective. The objective of this study was to verify the success of crosses on putative F1 soybean plants using SSR markers. The research was conducted from November 2018 to April 2019 at the Indonesian Center of Agriculture Biotechnology and Genetic Resources Research and Development in Bogor. The materials used in this study were varieties of Grobogan, Biosoy 1, and introduction accessions from the United States (PI 553045, PI 471938 and PI 471931), soil, "Karyana" cow manure, dolomite lime, 16-16-20 NPK fertilizer, gandasil D, gandasil B, Decis insecticide, paper label, yarn, fresh leaf samples, 2% PVP, CTAB (Cetyl Trimethyl Ammonium Bromide), sodium bisulfite 0.38%, sodium acetate (NaOAc) 3M, chloroform isoamylalcohol 24: 1, isopropanol, ethanol 70%, Tris-EDTA buffer (TE) 1x pH 8.0, KAPA2G Fast Ready Mix PCR Kit, ddH2O, agarose gel, Tris Asetat EDTA buffer (TAE) 1x pH 8.0, SYBR Gold Nucleic Acid Gel Stain, 1 kb DNA Ladder, molecular water (MW), 15 SATT primers for SSR-PCR, acrylamide-bisacrylamide 8%, Ammonium Persulfate (APS) 10%, TEMED, Mix electrophoresis, 1 kb marker, Tris Borate EDTA buffer (TBE) 1x pH 8.0 , tube 1.5 ml, tube 2 ml, and tissue KimWipes. While the tools used are 10 kg plastic pot, sprayer, tweezers, blue pestle, Erlenmeyer, measuring cup, micropipette, tips, incubator, water bath, centrifuge, tube rack, refrigerator, Speedvac DNA Concentrator, 96-plate tube, T1 Thermocycler (PCR), agarose gel plate, polyacrylamide gel plate, vertical electrophoresis, horizontal electrophoresis, tray, and UV Transilluminator. This research method consists of planting parents, crossing, F1 planting, DNA isolation, qualitative and quantitative DNA testing, PCR-SSR, polyacrylamide gel electrophoresis, and visualization of electrophoresis results. Observation variables consisted of the number of crossed flowers, the number of pods formed, thepercentage of successes in crosses, and the success of crosses from the results of molecular analysis. Data analysis was carried out by descriptive analysis. The results showed that verification of plant crosses using 5 selected primary pairs showed that of 65 putative F1 plants tested, as many as 15 verified plants were true F1 because they had DNA bands originating from a combination of the two parents' DNA bands. The most positive F1 was found in the population of Grobogan x Introduction 12 and Grobogan x Introductions 13, which were as many as 5 plants. While F1 positive is the least found in the Biosoy 1 x Introduction 10 population, which is as much as 2 F1 plants
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2019/383/051907105 |
| Subjects: | 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.3 Legumes, forage crops other than grasses and legumes > 633.34 Soybeans |
| Divisions: | Fakultas Pertanian > Budidaya Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 18 Aug 2020 03:01 |
| Last Modified: | 25 Oct 2021 02:00 |
| URI: | http://repository.ub.ac.id/id/eprint/173112 |
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