Sari, Kurnia (2018) Nanoenkapsulasi 2-Sitronelil Benzimidazol dengan Penyalut Kitosan-Tripolifosfat dan Uji Aktivitasnya sebagai Antibakteri. Sarjana thesis, Universitas Brawijaya.
Abstract
Benzimidazol telah dilaporkan dapat menghambat aktivitas pertumbuhan bakteri gram positif dan gram negatif seperti Staphylococcus aureus, Enteropatogenic Escherica coli (EPEC), dan Salmonella typhi. Nanoenkapsulasi 2-sitronelil benzimidazol merupakan teknik pengemasan bahan aktif yang memiliki keunggulan seperti mengendalikan pelepasan bahan aktif. Nanokapsul dibuat dengan bahan penyalut kitosan-tripolifosfat menggunakan metode ionik cross linking dan bahan aktif 2-sitronelil benzimidazol. Karakterisasi nanokapsul menggunakan Particle Size Analyzer (PSA) dan Zetasizer Nano, sedangkan penentuan jumlah bahan aktif yang tersaluti menggunakan Spektrofotometer UVVisible. Nanokitosan-tripolifosfat tanpa bahan aktif memiliki diameter partikel sebesar 95.180 nm sedangkan nanokapsul 2- sitronelil benzimidazol memiliki diameter partikel sebesar 159.410 nm. Hasil pengukuran Zetasizer Nano (ZS) menunjukkan nanokitosan-tripolifosfat tanpa bahan aktif memiliki ukuran 223,47 nm dan nanokapsul 2-sitronelil benzimidazol memiliki ukuran 3,293 nm. Penentuan bahan aktif yang dapat tersalut menggunakan spektrofotometer UV-Vis menunjukkan sebesar 56,76%. Penentuan kinetika pelepasan 2-sitronelil benzimidazol menunjukkan waktu paruh sebesar 86 menit 36 detik. Nanokapsul 2-sitronelil benzimidazol menunjukkan aktivitas antibakteri terhadap bakteri S. aureus memiliki zona hambat paling besar untuk waktu paruh 86 menit 36 detik sebesar 1,3 mm, EPEC memiliki zona hambat paling besar untuk waktu paruh 28 menit 53 detik sebesar 3,7 mm, dan S. thypi memiliki zona hambat paling besar untuk waktu paruh 86 menit 36 detik sebesar 5,7 mm.
English Abstract
Benzimidazole has been reported to inhibit the growth activity of gram-positive and gram-negative bacteria such as Staphylococcus aureus, Enteropatogenic Escherica coli (EPEC), and Salmonella typhi. 2-sitronelyl benzimidazole Nanoencapsulation is an active ingredient packaging technique that has advantages such as controlling the release of active ingredients. Nanocapsules were made with chitosan-tripolyphosphate coating material using ionic cross linking method and 2-sitronelyl benzimidazole active ingredient. Characterization of the nanocapsule using Particle Size Analyzer (PSA) and Zetasizer Nano, while the determination of the amount of active ingredient covered by UV-Visible Spectrophotometer. The tripolyphosphate non-active nanochitosan have a particle diameter of 95.180 nm while the 2-citronellyl benzimidazole nanocapsul has a particle diameter of 159,410 nm. The measurement results of Zetasizer Nano (ZS) showed that nanochitosan-tripolyphosphate without active ingredients had a size of 223,47 nm and 2-sitronelil benzimidazole nanocapsules had a size of 3,29 nm. Determination of active ingredients that can be coated using UV-Vis spectrophotometer showed 56.76%. Determination of 2-sitronelyl benzimidazole release kinetics showed a half-life of 86 minutes 36 seconds. 2-sitronelil benzimidazol nanocapsules showed that antibacterial activity against S. aureus bacteria had the largest inhibition zone for half-life of 86 minutes 36 seconds at 1,3 mm, EPEC had the largest inhibition zone for half-life of 28 minutes 53 seconds at 3,7 mm, and S. thypi has the largest inhibition zone for half-life of 86 minutes 36 seconds at 5,7 mm.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/381/051807956 |
| Uncontrolled Keywords: | nanokapsul, 2-sitronelil benzimidazol, cross-linking, kitosan-tripolifosfat, antibakteri, nanocapsule, 2-sitronelyl benzimidazole, cross-linking, chitosan-tripoliphosphate, antibacterial |
| Subjects: | 500 Natural sciences and mathematics > 541 Physical chemistry > 541.3 Miscellaneous topics in physical chemistry > 541.33 Surface chemistry > 541.34 Solution chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 29 Jul 2020 02:05 |
| Last Modified: | 22 Oct 2021 22:34 |
| URI: | http://repository.ub.ac.id/id/eprint/168801 |
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