-, Yuliatin (2018) Nanoenkapsulasi 2-Sitronelil Benzimidazol dengan Penyalut Kasein Misel dan Uji Aktivitasnya sebagai Antibakteri. Sarjana thesis, Universitas Brawijaya.
Abstract
Derivatif benzimidazol seperti 2-sitronelil benzimidazol diketahui memiliki aktivitas antibakteri terhadap bakteri gram positif Staphylococcus aureus dan gram negatif Escherichia coli. Nanoenkapsulasi didefinisikan sebagai proses dimana molekul bioaktif dikelilingi oleh lapisan, atau tertanam dalam matriks homogen maupun heterogen yang memiliki keunggulan dapat melindungi dari faktor luar seperti pH, oksigen dan cahaya. Nanokapsul 2-sitronelil benzimidazol dibuat dengan menggunakan penyalut kasein misel. Karakterisasi nanokapsul menggunakan Particle Size Analyzer (PSA) dan spektrofotometer Forier Transform Infrared (FT-IR) dan Scanning Electron Microscopy (SEM), sedangkan penentuan profil pelepasan dan penentuan jumlah bahan aktif yang terenkapsulasi menggunakan spektrofotometer UV-Vis. Nanokapsul 2-sitronelil benzimidazol memiliki ukuran rata-rata partikel 1017 nm. Adanya serapan gugus imina pada bilangan gelombang 1651,72 cm-1 spektra FT-IR menunjukkan bahan aktif sudah tersalut. Morfologi permukaan nanokapsul 2-sitronelil benzimidazol berbentuk trapesium dengan permukaan kasar dan tidak rata. Efisiensi enkapsulasi paling besar yaitu 79,97%. Penentuan profil pelepasan 2-sitronelil benzimidazol pada waktu pengadukan 15 hingga 45 menit terjadi peningkatan konsentrasi yang konstan, dan terjadi peningkatan yang signifikan pada waktu pengadukan 60 menit. Senyawa 2-sitronelil benzimidazol yang dilakukan enkapsulasi memiliki aktivitas antibakteri terhadap bakteri S. aureus dan E. coli dengan diameter zona hambat masingmasing 10 mm, dan 8,3 mm.
English Abstract
Benzimidazole derivative such as 2-citronellyl benzimidazole known to have antibacterial activity against grampositive bacteria of Staphylococcus aureus and gram negative of Escherichia coli. Nanoencapsulation is defined as a process in which bioactive compounds are surrounded by a coating,or embedded in a homogeneous or heterogeneous matrix which has the advantage of protecting from external factors such as pH, oxygen and light. 2- Citronellyl benzimidazole nanocapsule was prepared by using a casein micelle coating. Characterization of nanocapsule using Particle Size Analyzer (PSA) and Fourier Transform Infrared (FTIR) spectrophotometer and Scanning Electron Microscopy (SEM), while the determination of the release profile and determination of the amount of encapsulated active compound by using UV-Vis spectrophotometer. The 2-citronellyl benzimidazole nanocapsule has an average particle size of 1017 nm. The presence of imine group has wave number at 1651.72 cm-1 FT-IR spectra this band indicates the active compound has been coated. Surface morphology of 2- citronellyl benzimidazole nanocapsule is trapezoid with rough and uneven surface. The greatest encapsulation efficiency is 79.97%. Determination of 2-citronellyl benzimidazole release profile at stirring time of 15 to 45 minutes increased constant concentration, and there was a significant increase at 60 minutes stirring time. Moreover, 2-Citronellyl benzimidazole encapsulated has antibacterial activity against S. aureus, and E. coli with inhibitory zone diameters of 10 mm and 8.3 mm respectively.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/380/051807955 |
| Uncontrolled Keywords: | nanoenkapsulasi, 2-sitronelil benzimidazol, kasein misel, antibakteri, nanoencapsulation, 2-citronellyl benzimidazole, casein micelle, antibacterial |
| Subjects: | 500 Natural sciences and mathematics > 541 Physical chemistry > 541.3 Miscellaneous topics in physical chemistry > 541.33 Surface chemistry > 541.34 Solution chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 28 Jul 2020 08:01 |
| Last Modified: | 22 Oct 2021 22:33 |
| URI: | http://repository.ub.ac.id/id/eprint/168798 |
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