Septiana, Pretty (2018) Pengaruh Pemberian Ekstrak Etanol Akar Pletekan (Ruellia tuberosa L.) terhadap Aktivitas Enzim Protease dan Profil Protein pada Pankreas Tikus Putih (Rattus norvegicus) Diabetes Melitus Tipe 1 akibat Induksi MLD-STZ. Sarjana thesis, Universitas Brawijaya.
Abstract
Diabetes Melitus (DM) merupakan penyakit gangguan metabolisme yang ditandai dengan meningkatnya kadar glukosa (Hiperglikemia) akibat gangguan sekresi insulin dan atau resistensi insulin. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol akar pletekan (Ruellia tuberosa L.) terhadap pankreas tikus Diabetes Melitus. Parameter yang diamati adalah aktivitas protease dan profil protein dari organ pankreas tikus. Pada penelitian ini, tikus putih (Rattus norvegicus) di induksi MLD-STZ dosis 20 mg/kgBB selama 5 hari berturut-turut. Tikus putih (Rattus norvegicus) sejumlah 20 ekor dibagi menjadi 5 kelompok yaitu kontrol negatif, kontrol positif, kelompok terapi dengan ekstrak etanol akar pletekan menggunakan dosis 250, 375, 500 mg/kgBB secara oral selama 21 hari. Aktivitas protease diukur dengan metode spektrofotometri UV dan analisa profil protein dengan metode elektroforesis SDS-PAGE. Hasil penelitian menunjukkan bahwa ekstrak etanol akar pletekan memberikan perbedaan yang sangat nyata (p<0,01) dalam menurunkan aktivitas protease pankreas tikus DM sebesar 52,11% pada dosis terbaik 250 mg/kgBB. Pada hasil analisa profil protein pemberian ekstrak etanol akar pletekan memberikan pengaruh hilangnya protein dengan berat molekul 52 kDa yang muncul pada tikus kontrol positif. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak etanol akar pletekan (Ruellia tuberosa L.) dapat digunakan menurunkan aktivitas protease dan memperbaiki profil protein pada pankreas tikus DM.
English Abstract
Diabetes mellitus (DM) is a disease with metabolism disorder signed by increasing of blood glucose (Hyperglycemia) caused by a disorder of insulin secretion and or insulin resistance. This study aims to determine the influences of ethanol Ruellia tuberosa L. roots extract in pancreas Diabetes Mellitus rat. The parameters observed in this study were protease activities and protein profile of diabetic rat’s pancreas. In the current work, white rats (Rattus norvegicus) were induced with the MLD-STZ dose of 20 mg/kgBW for 5 days successively. White rats (Rattus norvegicus) as many as 20 rats which were divided into 5 groups, they were one group of negative control, one group of positive control and three group of therapeutic ethanol Ruellia tuberosa L. roots extract with dose 250, 375, 500 mg/kgBW for 21 days. Protease activities were measured by spectrophotometric UV method and protein profile analysis was performed by electrophoresis SDS-PAGE method. The results showed that ethanol extract of Ruellia tuberosa L. roots showed a very real difference (p<0,01) to decrease protease activity of rat’s pancreas DM equal to 52,11% with the best doses 250 mg/kgBW. Protein profiles showed that ethanol extract of Ruellia tuberosa L. roots affected the loss of protein with 52 kDa molecular weight that appeared in positive control rats. Based on this research, it can be concluded that ethanol extract of Ruellia tuberosa L. roots can be used to decrease protease activity and repaired protein profile in pancreas of DM rats
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/346/051807399 |
| Uncontrolled Keywords: | Aktivitas Protease, Diabetes Melitus (DM), Pankreas, Pletekan (Ruellia tuberosa L.), Profil Protein, Streptozotocin, Diabetes Mellitus (DM), Pancreas, Pletekan ( Ruellia tuberosa L.), Protease Activities, Protein profile, Streptozotocin. |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.4 Diseases of endocrine, hematopoietic, lymphatic, glandular system; diseases of male breast > 616.46 Diseases of islands of Langerhans > 616.462 Diabetes mellitus > 616.462 2 Diabetes mellitus, type 1 (Insulin-dependent diabetes) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 02 Jun 2020 06:24 |
| Last Modified: | 22 Oct 2021 22:25 |
| URI: | http://repository.ub.ac.id/id/eprint/168698 |
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