Rismawati, Vivi (2018) Pengaruh Pemberian Kompleks Scavenger Aoc (Aspirin-Ovalbumin-Caffeine) Terhadap Perbaikan Mukosa Lambung Tikus Putih (Rattus Norvegicus) Gastritis. Sarjana thesis, Universitas Brawijaya.
Abstract
Gastritis merupakan inflamasi pada mukosa lambung. Penelitian ini bertujuan mengetahui pengaruh kompleks scavenger AOC (AspirinOvalbumin-Caffeine) terhadap perbaikan struktur mukosa lambung tikus putih (Rattus norvegicus) gastritis. Penelitian dilakukan pada tikus putih betina galur wistar dengan delapan kelompok perlakuan, tiga ulangan. Kelompok kontrol negatif (umur tikus 1,5 bulan) K0 disonde 2 mL air mineral dan K1 disonde 2 mL larutan AOC selama 30 hari. Kontrol positif K2 umur 1,5 bulan dan K3 umur 6 bulan disonde larutan aspirin dosis tunggal 300 mg/kg BB tikus untuk stimulasi gastritis akut. K2- AOC tikus gastritis akut umur 1,5 bulan tanpa AOC tujuh hari. K2+AOC tikus gastritis akut umur 1,5 bulan di-treatment 2 mL AOC tujuh hari. K3-AOC tikus gastritis akut umur 6 bulan tanpa AOC. K3+AOC tikus gastritis akut umur 6 bulan di-treatment 2 mL AOC tujuh hari. Pengamatan morfologi lambung meliputi bleeding spot (ada atau tidak, jumlah, lokasi, ciri-ciri). Pengamatan preparat histologi lambung meliputi perubahan surface epithelium, nekrosis sel parietal di gastric glands, ekstravasasi eritrosit, hemorrhage, infiltrasi sel radang polimorfonuklear (PMN) pada gastric glands dan muscularis mucosae di mukosa lambung yang diamati menggunakan mikroskop cahaya Olympus DP73 dengan bantuan software cellsens standard pada perbesaran 400x dan 1000x. Data dianalisis secara deskriptif. Erosi mukosa lambung terjadi pada kelompok kontrol positif gastritis umur 1,5 bulan yakni ada 22 bleeding spots focal dan pada kelompok kelompok kontrol positif gastritis umur 6 bulan yakni erosi dengan dasar bersih. Kelompok gastritis mengalami kerusakan epitel, ekstravasasi eritrosit, infiltrasi PMN, hematoma, dan nekrosis. Bleeding hanya terjadi pada kelompok gastritis umur 1,5 bulan. Kelompok gastritis yang ditreatment AOC terjadi indikasi re-epitelisasi dan inflamasi berkurang. Hal tersebut tidak terjadi pada kelompok gastritis tanpa treatment AOC
English Abstract
Gastritis is an inflammation on gastric mucosal. Research aims to know effect of AOC (Aspirin-Ovalbumin-Caffeine) scavenger complex to repair structure of gastric mucosal rat (Rattus norvegicus) gastritis. Research was performed on female wistar strain rats using eight groups, three replications. Negative control groups (rat aged 1.5 months) K0 were feeding with 2 mL mineral water and K1 were feeding with 2 mL AOC solution for 30 days. Positive control groups K2 (aged 1.5 months) and K3 (aged 6 months) were feeding with single dose of aspirin solution 300 mg/kg W rat to stimulate acute gastritis. K2-AOC as acute gastritis rat (aged 1.5 months) were not treated with AOC for seven days. K2+AOC as acute gastritis rat (aged 1.5 months) were treated with 2 mL AOC seven days. K3-AOC as acute gastritis rat (aged 6 months) were not treated with AOC. K3+AOC as acute gastritis rat (aged 6 months) were treated with 2 mL AOC seven days. Gastric morphology observation include bleeding spots (existence, number, location, characteristic). Gastric histology observation include changes on surface epithelium, necrotic cell, erythrocytes extravasation, hemorrhage, polymorphonuclear (PMN) inflammatory cells infiltration on gastric glands and muscularis mucosae in gastric mucosal layer. It was observed by using Olympus DP73 light microscope with cellsens standard software at 400x and 1000x magnification. Data were analyzed descriptively. Gastric morphology erosion occurs on gastritis positive control group (aged 1,5 months) ie erosion with 22 bleeding spots focal and occurs on gastritis control group (aged 6 months) ie erosion with clean base. Gastritis groups have epithelial damage, erythrocytes extravasation, PMN infiltration, hematoma, and necrosis. Bleeding only occurs on gastritis group (aged 1,5 months). Gastritis groups that were treated with AOC there were indication of re-epithelization, reduction of inflammatory area. It does not happen on gastritis groups without AOC.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018.337/051807390 |
| Uncontrolled Keywords: | AOC, aspirin, gastritis, histologi, Rattus norvegicus, AOC, aspirin, gastritis, histology, Rattus norvegicus |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.07 Pathology > 616.075 Diagnosis and prognosis > 616.075 6 Chemical > 616.075 63 Analysis of gastroenteric contents |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 03 Jun 2020 12:31 |
| Last Modified: | 22 Oct 2021 13:53 |
| URI: | http://repository.ub.ac.id/id/eprint/168648 |
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