Fauzi, Mochammad Imam (2018) Pengaruh Pemberian Benzo[a]pyrene terhadap Profil CD4+, CD8+, B220, dan IFN-α pada Mencit (Mus musculus) yang Divaksin Campak. Sarjana thesis, Universitas Brawijaya.
Abstract
Cakupan vaksin campak di Indonesia telah mencapai 90%, namun tingkat kekebalan masyarakat hanya pada kisaran 81%. Penurunan kekebalan tersebut dapat disebabkan oleh beberapa faktor salah satuya adalah adanya senyawa seperti benzo[a]pyrene. Tujuan penelitian ini yaiu untuk mengetahui pengaruh dan seberapa besar potensi benzo[a]pyrene dalam menurunkan ekspresi CD4+, CD8+, B220, dan IFN-α pada mencit yang divaksin campak. Penelitian dilakukan dengan cara ditentukan empat kelompok perlakuan yaitu kontrol, vaksin, benzo[a]pyrene, dan vaksin+benzo[a]pyrene. Vaksin 20 mg/kg BB diinjeksikan satu kali pada awal perlakukan dan benzo[a]pyrene (20 mg/kg BB) diinjeksikan tiga hari sekali selama lima minggu. Sementara itu, ekspresi CD4+, CD8+, B220, dan IFN-α ditentukan menggunakan analisis flow cytometry melalui sel yang diisolasi dari limpa. Hasil kemudian diuji secara statistik melalui uji ANOVA one way dan dilanjutkan dengan uji Tukey. Hasil menunjukkan B[a]P tidak berdampak terhadap penurunan ekspresi IFN-α pada sel T CD4+ maupun pada sel T CD8+ baik pada kelompok perlakuan B maupun VB. Sementara itu, injeksi B[a]P pada dosis 20 mg/kg BB diduga mampu meningkatkan aktivitas NF kB sehingga ekspresi CD25+ pada sel B meningkat. Terdapat peningkatan rasio B220+:CD4+, hal ini diduga karena B[a]P mampu menurunkan populasi CD4+ sehingga jarak populasi sel B220+ dan sel CD4+ meningkat. Dari penelitian ini dapat dikatakan bahwa efek B[a]P selektif terhadap parameter tertentu.
English Abstract
The coverage of measles vaccine in Indonesia has reached 90%, but immunity level of community is only in the range of 81%. The decrease in immunity may be caused by several factors such as the presence benzo[a]pyrene. The purpose of this study was to determine the effect and potential of benzo[a]pyrene in decreasing the expression of CD4+, CD8+, B220, and IFN-α in measles vaccinated mice. The study was conducted by determined four treatment groups: control, vaccine, benzo[a]pyrene, and vaccine+benzo[a]pyrene. The 20 mg/kg vaccine was injected once and benzo[a]pyrene (20 mg/kg BW) was injected each three for five weeks. Meanwhile, the expression of CD4+, CD8+, B220, and IFN-α was determined using flow cytometry analysis through isolated the cells from spleen. The results then tested statistically by one way ANOVA and continued with the Tukey test. The results showed that B[a]P had no effect on decreasing IFN-α expression in CD4+ T cells nor on CD8+ T cells either in the B treatment group or VB. Meanwhile, the injection of B[a]P at dose of 20 mg / kg BW could increase the activity of NF kB so that CD25+ expression in B cells was increased. There is an increase in the B220+:CD4+ ratio, presumably because B[a]P is able to decrease the CD4 population so that the population of B220 and CD4+ T cells increases. From this research it can be said that the effect of B[a]P is selective towards a particular cell as well as the potential of B[a]P to influence the profile on each parameter depending on the given dose.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/164/051805310 |
| Uncontrolled Keywords: | Benzo[a]pyrene, B220, CD4+, CD8+, IFN-α, Vaksin campak. Benzo[a]pyrene, B220, CD4+, CD8+, IFN-α, Measles vaccine. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.37 Immunologic drugs and immune serums > 615.372 Vaccines |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 03 Jun 2020 10:38 |
| Last Modified: | 18 Oct 2021 02:51 |
| URI: | http://repository.ub.ac.id/id/eprint/168609 |
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